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Citations for Roche :
4451 - 4500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: Total RNA was isolated and purified using TriPure Isolation Reagent (Roche), and cDNA was synthesized from 1 µg of total RNA using Verso cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Genetics 2024Quote: ... using LightCycler 480 Instrument II (Roche). mRNA levels for each experimental group were quantified using the comparative CT method.
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910). For in situ detection ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1mg/ml Papain (Roche, 10108014001) for 10 minutes at 37°C with trituration every 2 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... TMR red (Roche, 12156792910) was performed on e14.5 paraffin sections ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with DAPI (0.5 µg/ml, Roche, Basel, Switzerland). Images were acquired using either a Zeiss AxioObserver epifluorescence microscope with Apotome ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted using the Tripure Reagent and treated with DNase (both from Roche). The resulting RNA was reverse transcribed with the iScriptTM cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... The enzymes ExpandTM High Fidelity PCR System (Roche) or Phusion® High-Fidelity DNA Polymerase (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10 µM MG132) containing the EDTA-free protease inhibitor cocktail (Roche). After 15 min of incubation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by addition of 100μl DNase I (10 mg/ml; Roche) and 100μl FBS (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... using Light cycler 480 SYBR Green I Master mix 2X (Roche) in 96-well plates at 95°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification of first-strand cDNAs was performed on the LightCycler 480 Real-Time PCR system (Roche), using Light cycler 480 SYBR Green I Master mix 2X (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification of first-strand cDNAs was performed on a LightCycler 480 Real-Time PCR system (Roche) in 96-well plates at 95 °C for 2 min and 45 cycles of 95 °C for 10 s and 56 °C for 60 s ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-β-galactosidase (1:1000, Roche), rabbit anti- GPR158 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Triton X-100 supplemented with protease inhibitors (Roche). Media was removed from each well and replaced with STEN buffer ...
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bioRxiv - Neuroscience 2024Quote: ... anti-c-myc (Roche, #11667149001, 1:200), anti-MAP-2 (H-300 ...
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bioRxiv - Physiology 2024Quote: ... fixed with 4% PFA for 15 min and stained with 4′,6-diamidino-2-phenylindole (DAPI) (0.1 µg/mL; Roche, cat no. 10236276001) for 10 min at RT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Blocking was performed in MAB blocking buffer (100 mM Maleic acid, 150 mM NaCl, 2% Blocking reagent [Roche, 1096176] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and DIG RNA Labeling Mix (Roche 11277073910). The reaction was incubated for 2–4 h at 37°C ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... after capture of polyadenylated RNA using the KAPA mRNA HyperPrep kit (#KR1352-v7.21; Roche). The individual libraries were assessed using a Qubit™ and the Agilent Bioanalyzer dsDNA High Sensitivity Kit (Agilent Technologies) ...
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bioRxiv - Genetics 2024Quote: ... RNA sequencing libraries were prepared using the KAPA mRNA HyperPrep Kit V2 (Roche). Paired-end 100x100 bp sequencing was performed on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... Minas Gerais) in an automated biochemical analysis equipment (Cobas Mira S, Roche®, Basel, Switzerland).
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bioRxiv - Physiology 2024Quote: RNA-seq library preps were generated using the Kapa stranded mRNA-seq kit (Roche, Basel, Switzerland). Samples were individually barcoded sequenced on the Illumina Hi-Seq 4000 (Illumina Inc ...
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bioRxiv - Synthetic Biology 2024Quote: DNase I digestion was performed using DNase I (Roche 4716728001). We mixed 15 µl of the RNA sample with 20U of DNase I in 50-µl reaction ...
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bioRxiv - Genetics 2024Quote: ... and qPCR was carried out using the FastStart Essential DNA Green Master (Roche) in a CFX Connect Real-Time System (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... and 1x KAPA Hifi Hotstart ReadyMix (KAPA Biosystems, Wilmington, MA)] ...
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bioRxiv - Neuroscience 2024Quote: ... and 50mM HEPES (pH 7.4) supplemented with protease inhibitors (Roche) for 45 minutes at 4°C while gently shaking ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5μL SYBR Green (Roche, Switzerland) per well ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All the libraries were double-indexed using KAPA HiFi HotStart Uracil+ReadyMix (Roche). The resulting indexed libraries were quantified on a QubitTM dsDNA HS (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... the digestion was stopped by adding 0.5 mg/mL DNase I (Roche) in microglia medium (DMEM (PAN Biotech) ...
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bioRxiv - Neuroscience 2024Quote: ... The EdU Click-iT reaction solution (Roche) was prepared according to manufacturer’s instructions and larvae were incubated at room temperature in the dark for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The sugar-free HL3-like saline was added with 30 units of Papain (Roche) and applied to the head capsule for 10 min to digest the brain’s glial sheath and facilitate removal ...
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bioRxiv - Neuroscience 2024Quote: ... 4 mg/mL DNAase (Roche, 04716728001). The cells were resuspended by mechanical agitation through Pasteur pipettes flamed with decreasing diameters ...
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bioRxiv - Biochemistry 2024Quote: ... and a micro-spatula full of DNAse (Roche, #10104159001). Cells were lysed on ice by sonication (3 secs on ...
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bioRxiv - Biochemistry 2024Quote: ... End point cell proliferation was assayed with ELISA 5-bromo-2′ -deoxyuridine (BrdU) kit from Roche Diagnostics (Sigma– Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the three different cell types were assayed for telomerase activity according to the TeloTAGGG Telomerase PCR ELISA Kit (11854666910; Roche).
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bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNase I (Roche, #11284932001) for 45 minutes at 37°C undergoing constant ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were generated from minced lung tissue that was subjected to collagenase (Liberase TM Research Grade, Roche), hyaluronidase (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche Cat# 0796014001) and sequenced at a targeted depth of 25,000 reads per cell ...
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Immunology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (2 tablets/100 ml of serum; Roche #11873580001) and 8 ml of 500 mM CaCl2 ...
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bioRxiv - Immunology 2024Quote: ... gel bands corresponding to the LRP1 protein were excised and the N-glycans were attained from an overnight in-gel N-glycan release with 2U PNGaseF (Roche) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X cOmplete™ protease inhibitor EDTA-free cocktail (Roche, 1187358001). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Microbiology 2024Quote: ... was used to amplify cDNAs in a mix of 1x Lightcycler® 480 SYBR Green I Master Mix (Roche) and 0.2 µM of a series of sequence-specific primer pairs (see Table 1 for detailed target sequences) ...
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bioRxiv - Microbiology 2024Quote: ... The NGS library was quantified with a qPCR-based KAPA Library Quantification kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Briefly ...