Labshake search
Citations for Roche :
4451 - 4500 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: ... The filters were washed with 130 μl 50 mM ammonium bicarbonate and centrifuged again before overnight digestion with 100 μl 5 ng/μl trypsin (Roche) at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... in 48-well plates and transfected at 50-60% confluence with a total of 100 ng Dual-pMIR-report plasmids using X-tremeGENE 9 DNA Transfection Reagent (Roche), followed by transfection with mimics (Life Technologies ...
-
bioRxiv - Microbiology 2019Quote: ... washed in cold PBS and resuspended in 100 – 300 μl DI-water with 1mM PMSF and cOmplete EDTA-free protease inhibitor tablet (Roche). Resuspended pellets were freeze-thawed three times with liquid nitrogen/37°C water bath ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then, spikelets were dehydrated through a series of ethanol (50%, 70%, 85%, 95%, and 100%) and embedded in paraffin (Roche). Cross-sections of 10 µm thickness were cut using the rotary microtome followed by a dehydration gradient of 95% ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... rRNA-depleted RNA-seq libraries were prepared from 100-1,000ng RNA per sample using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, #KK8560). RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina ...
-
bioRxiv - Cell Biology 2020Quote: POT-2 expression pellets were resuspended in Tris buffer (50 mM Tris/HCL pH 7.5, 100 mM NaCl, 10 mM MgCl2, 1x EDTA-free protease inhibitor (Roche, #4693132001)) and divided into 2 mL flat lid micro tubes containing 0.1 mm zirconia beads (Carl Roth ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cells were lysed in 300 μl Triton X-100 lysis buffer supplemented with EDTA-free protease inhibitor mix (Roche). 100 μl supernatant and 100 μl diluted cell lysates were independently incubated with 100 μl AP substrate p-nitrophenyl phosphate (PNPP ...
-
bioRxiv - Immunology 2022Quote: ... Precipitated pellet was then resuspended in 100 μL of RIPA buffer plus laemli supplemented with 5 mM diisopropylfluorophosphate (DFP) and protease inhibitor cocktail (Roche) and heat denaturated for 10 min at 95°C ...
-
bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed followed by the pellet being triturated in 100 μL of lysis buffer (7M Urea, 2M Thiourea, 20mM Tris-HCl) and 1x protease inhibitor (Roche cOmplete mini-inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). The lysates were then resuspended in 5 x SDS-PAGE loading buffer containing 25mM DTT ...
-
bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in nuclear extraction buffer (20mM HEPES pH7.5, 10% glycerol, 350mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) and incubated for 30 min at 4°C on a rotator ...
-
bioRxiv - Molecular Biology 2020Quote: ... The crude nuclear pellet was rinsed in wash solution (20mM HEPES pH7.5, 10% glycerol, 150mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) twice ...
-
bioRxiv - Microbiology 2021Quote: ... and 100 ng of DNA was converted into paired-end Illumina sequencing libraries using KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche) ...
-
bioRxiv - Genomics 2022Quote: ... The eluted material and input were incubated for 6 h at 65°C to reverse crosslinks and were treated with 100 ng RNaseA (Roche) for 1 h at 50°C ...
-
bioRxiv - Immunology 2019Quote: ... Lysis buffer was added (40 mM HEPES, 140 mM NaCl, 0.1% Triton-X-100, Roche EDTA-free complete protease inhibitor) and cells were detached with a cell scraper ...
-
bioRxiv - Neuroscience 2019Quote: ... Following permeablisation in PBSTx (0.3% Triton 100 X in PBS) EdU labelling was detected by incubating tails in fresh EdU Click-iT reaction solution (Roche) for 2 hours in the dark at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... were added (0.5 g to each 100 mg pellet) along with protease inhibitor solution (complete mini EDTA-free protease inhibitor cocktail, Roche), dH2O and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
-
bioRxiv - Developmental Biology 2020Quote: ... of 90-100% confluent HEK293T cells were lysed directly using CERI and NER buffer supplemented with 1x protease inhibitor cocktail (Roche), 1x PhosSTOP (Roche) ...
-
bioRxiv - Molecular Biology 2019Quote: Batches of ten 2 day-old adult flies were crushed in 100 µl of lysis buffer (0.3% SDS in PBS plus one EDTA-free cOmplete™ Protease Inhibitor Cocktail tablet, Roche), incubated for 15 min and centrifuged at 15,000 gmax for 10 min (all manipulations at room temperature) ...
-
bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor). To this 4ul of Benzonase (E1014 ...
-
bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor), 2ul of Benzonase (E1014 ...
-
bioRxiv - Biochemistry 2020Quote: Cells were washed twice with ice-cold PBS and lysis in ice-cold 100 mM sodium carbonate containing protease inhibitor cocktail (Roche) by tip-probe sonication ...
-
bioRxiv - Physiology 2020Quote: 100 µL of whole blood was pelleted by rapid centrifugation and pellets were lysed in 100 µL of RIPA lysis buffer with proteinase inhibitor cocktail (Roche). Following an additional centrifugation step ...
-
bioRxiv - Neuroscience 2020Quote: ... and lysed by the addition of 100 μl of ice-cold RIPA lysis buffer (Beyotime) with protease inhibitor cocktail (Roche). Cellular debris was removed by centrifugation at 13,000 g for 15 min at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: The retina was homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Biochemistry 2021Quote: ... retinas were homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Coverslips were immediately moved to ice cold cytoskeletal buffer (10mM PIPES pH 6.8, 300mM sucrose, 50mM NaCl, 3mM EDTA, 0.5% Triton X-100, Protease Inhibitor Cocktail [cOmplete™ EDTA-free; Roche, 27368400]) for 5 minutes before fixation in 2% PFA ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and lysed in HEPES buffer supplemented 100 μM N-ethylmaleimide and protease inhibitor cocktail (Roche). The lysates were centrifuged and incubated with 2 μg Mcl-1 antibody (S-19 ...
-
bioRxiv - Cancer Biology 2022Quote: 100 to 500 ng of total RNAs were reverse-transcribed with Transcriptor First Strand cDNA synthesis kit (Roche, Basal, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
-
bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
-
bioRxiv - Plant Biology 2022Quote: ... total proteins were extracted with lysis buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 100 mM PMSF, and 1X Roche protease inhibitor cocktail), followed by incubated with anti-GFP beads (AB_2631358 ...
-
bioRxiv - Plant Biology 2022Quote: Total protein extracts were prepared by re-suspending 100 mg of ground plant material in 100 μl of protein lysis buffer (50 mM Tris pH 8.0; 2% SDS; 10 mM EDTA; protease inhibitors (Roche (04693159001) cOmplete™ ...
-
bioRxiv - Neuroscience 2022Quote: ... 50□mM potassium acetate, 200□mM sorbitol, 2_mM EDTA, 0.1% Triton X-100, 1X Complete Mini protease inhibitor cocktail [Roche Applied Sciences]), allowed to rotate for 1 h at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: RNA-seq libraries were constructed by the ICM Platform (PARIS) from 100 ng of total RNA using the KAPA mRNA Hyperprep kit (Roche) that allows to obtain stranded polyA libraries ...
-
bioRxiv - Molecular Biology 2022Quote: ... The loci of interest were first amplified with 15 cycles of PCR from 2 μL (∼100 ng) of genomic DNA eluate using a 5 μL Kapa HiFi HotStart polymerase reaction (Roche). The first PCR was then diluted with 25 μL of DNAse-free water ...
-
bioRxiv - Microbiology 2024Quote: ... and 100 ng were used for paired-end Illumina sequencing library preparation using the KAPA HyperPlus Library Preparation Kit (Roche) and the KAPA Unique Dual-Indexed Adapater Kit (Roche) ...
-
bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...
-
bioRxiv - Genetics 2023Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche), as described previously (Kemaladewi et al. ...
-
bioRxiv - Genomics 2023Quote: ... and resuspended in 1 mL Nuclear Isolation Buffer (20mM HEPES, 10mM KCl, 1mM MgCl2, 0.1% Triton X-100, 20% Glycerol, and 1X Protease Inhibitor (Roche 4693132001)) per 10 million cells by gently pipetting 5x with a wide-bore pipette tip ...
-
bioRxiv - Systems Biology 2023Quote: ... The resulting polysome pellet was briefly rinsed with 500 µL ice-cold diethyl pyro carbonate (DEPC)-treated water and resuspended overnight in 100 µL ice-cold ribosome buffer (2x concentrated TKM+ buffer diluted with 25x concentrated Roche protease inhibitor cocktail to a total amount of 16% ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenized in a ‘homogenization buffer’ (Sucrose, 100 mM EGTA, 10 mM Tris, protease and phosphatase inhibitors (Roche), pH 8.1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 g of green thallus was harvested in Galbraith buffer pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany) on ice ...
-
bioRxiv - Molecular Biology 2023Quote: ... the supernatant was removed and the pellet was resuspended in 250 µL of Galbraith buffer pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). 50 µL of 0.2 µm glass beads were added and samples were vortexed for 3 minutes to smash open spores and release nuclei ...
-
Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... The bacterial pellets were dispersed in lysis buffer of 8 M urea in 100 mM ammonium bicarbonate and protease inhibitor cocktail (CoMplete, (Roche)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... nuclei were isolated from 1×105 cells by incubating them in the NE Buffer (20 mM HEPES-KOH, pH 7.9, 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 0.5 mM Spermidine, 1x Roche cOmpleteTM Protease Inhibitor) for 10 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
-
bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...