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Citations for Roche :
4401 - 4450 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000; Roche 12CA5 HA at 1:2,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 mM KCl, 0.05 mM EDTA, 10% Glycerol, 1 mM DTT, 1 mM PMSF, 1× Complete Mini protease inhibitor, Roche) resuspended in RIPA buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The nuclei pellet was lysed in 400 μl RIPA buffer 1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) and incubated on ice for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... and LiCl (250 mM LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 20 mM Tris-HCl PH8.0, complete protease inhibitor [Roche]) washing buffers ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). 20µl of HPC4 beads were supplied with 1mM Ca2+ ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). Clarified extracts were incubated for 2 h with agarose beads previously coupled to 10 µg of non-immune rabbit IgG or 10 µg of affinity purified Arpin antibodies (according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). Extracts were incubated with anti-GFP agarose beads (GFP-trap ...
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bioRxiv - Neuroscience 2020Quote: ... γ at a 1:1:1 ratio) using retro-inverso dioleoylmelittin (riDOM) 0.2 mg/nl (synthesized at Hoffman-La Roche) and PEI 25 kD 0.67 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the nonspecific staining was blocked with 10% normal donkey serum in PBS with 1% BSA and 0.3% triton for 1 hour and then incubated with mouse anti-BrdU (1: 1000, Roche), rabbit anti-Ki67 (1 ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... initial denaturation at 95°C for 3 min, followed by 40 cycles of amplification (95°C for 15 sec, 60°C for 30 sec) (Roche LightCycler 96, Roche Diagnostics). For absolute quantification ...
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bioRxiv - Microbiology 2024Quote: ... and phoC-A-R1 (5’-CAA CAT CGC TTT GCC AGT G-3’) (Fraser et al., 2017) with a Roche LightCycler® 96 (Roche Diagnostics, Mannheim, Germany) using 5 µL KAPA SYBR FAST 2x qPCR Master Mix (KAPA Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 10 minutes at 37°C in a 3% (w/v) collagenase A solution in PBS (Collagenase A, Roche Diagnostics GmbH, Germany, 10103586001). Cells were recovered by centrifugation for 5 minutes at 300g prior cell counting on Malassez cells after Trypan blue staining ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in PBS-E LB (1x PBS, 1% TX100, 1 mM EDTA, 1 mM DTT, Protease Inhibitor cocktail (Roche) and Phosphatase Inhibitor solution (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... Heads were homogenised in 1 mL BRB-80 lysis buffer (80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, cOmplete EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
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bioRxiv - Developmental Biology 2023Quote: Spinal cords from wt and Sorcs2-/- mice were dissected and lysed on ice with TNE lysis buffer (Tris-base 10 mM, NaCl 150 mM, EDTA 1 mM, NP40 1%, cOmplete 1% (Roche), PhosSTOP 1% (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 M urea, 0.5% SDS, 1 mM EDTA pH8.0, 1 mM DTT, and 1× protease inhibitor cocktail [Roche, cat#4693132001]). Biotinylated RBPs were captured by affinity purification ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 1 Complete Mini Protease Inhibitor Cocktail tablet (Roche) were added to 10 mL buffer ...
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bioRxiv - Microbiology 2020Quote: ... anti-3xHA (1:2000, 12CA5, Roche or 1:4000, C29F4, Cell Signaling) anti-PfALD (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 1° antibodies used: α-GFP 1:1000 dilution (Mouse, Roche, Cat.No. 11814460001), α-Tubulin (TAT1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 % (v/v) NP40 and 1 x cOmplete Protease inhibitor cocktail (Roche)) by incubating on ice for 30 min ...
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bioRxiv - Genomics 2021Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland) and eluted in Tris-Cl ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA and supplemented with 1 × complete protease inhibitor cocktail (Roche). DDM/CHS detergent (1%/0.1% ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland). Genomic libraries were generated using Kapa Hyper library prep kit (Roche ...
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bioRxiv - Genomics 2023Quote: ... 1 mM PMSF and 1×cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche) were added throughout the above procedures ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After incubation for 1-2 hours in a 1% blocking solution (Roche) dissolved in 1× PBS ...
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bioRxiv - Genetics 2023Quote: ... 1 mM EDTA and 1 x cOmpleteTM mini protease inhibitor cocktail (Roche). PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... with mRNA enrichment from 100 ng of total purified RNA and Illumina sequencing libraries preparation performed using Kapa stranded mRNA Hyper Prep (Roche Sequencing Solutions ...
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bioRxiv - Cell Biology 2020Quote: ... Pelleted cells were resuspended in lysis buffer (100 mM Sodium Phosphate pH 7.0, 300 mM NaCl) containing protease inhibitors (Complete™, Roche) and sonicated ...
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bioRxiv - Immunology 2021Quote: ... S1 protein and CP15 were formulated in nanoparticles in PLGA (Alchem Laboratories) for the first 2 doses and the last (one-year) boost was in DOTAP (100 μl per dose; Roche). For immunization ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Chromo-1 transcriptome sequencing was performed on the Illumina HiSeq platform (UCLA Clinical Microarray Core) with read lengths of 100 bp using the KAPA stranded RNA-seq kit (Roche) to construct paired-end libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Genomics 2020Quote: ... Samples were pooled (150 ng of each reference samples and 100 ng of other samples) and the final library concentration was determined by qPCR (KAPA Library Quantification Kit, Roche). Sequencing was performed on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Cell Biology 2019Quote: 10 heads from animals at 8 DAE were resuspended in 100 μl of Hepes/NaOH 50mM with proteases and phosphatases inhibitors (Roche), samples were disrupted using a mechanical tip following two rounds of sonication of 10 sec each ...
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bioRxiv - Genetics 2019Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche). Cells were subsequently incubated on ice for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Neuroscience 2020Quote: ... frozen tissue was mechanically homogenized in TES buffer (4% Tris Base pH 7.5, 0.1 mM EDTA, 100 mM Na2Cl) containing protease inhibitor (PI) (cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany)) with the VDI 12 homogenisator (VWR ...