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Citations for Roche :
4701 - 4750 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: The cell pellet was resuspended in 500 μL of cold permeabilization buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% IGEPAL CA-630, 100 μL of protease inhibitor cock-tail Roche Cat no. 11836170001) and placed on ice for 15 min ...
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bioRxiv - Biophysics 2019Quote: ... Pelleted cells were resuspended in Buffer A (20 mM Tris pH 7.5, 100 mM NaCl) with cOmplete EDTA-free protease inhibitor (Roche, Indianapolis, IN), and lysed using an Avestin C5 emulsifier at ∼15,000 psi ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed (10mM Tris-HCl [pH 8], 0.25% Triton X-100, 10mM EDTA, 100mM NaCl, Roche 1X Complete Protease Inhibitor) and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini, EDTA-free Protease Inhibitor Cocktail (Roche, #04693159001)) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in 100 μl cold RIPA buffer (Pierce, 8990) supplemented with cOmplete mini EDTA-free protease inhibitor cocktail (Roche, 11836170001). Protein abundance was measured by Qubit ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei from 600,000 cells were isolated by hypotonic lysis in 1 mL buffer NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 0.5 mM spermidine; 0.1% Triton X-100; 20% Glycerol; Roche EDTA-free protease inhibitor) for 10 minutes on ice followed by centrifugation at 1300 xg for 4 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified using 22 cycles of PCR amplification from approximately 300 µg genomic DNA (10 µg DNA/100 µL reaction) using 2X KAPA HiFi HotStart ReadyMix (Roche KK2602), a combination of 8 staggered P5 primers ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were then isolated by incubating with NE1 buffer (10 mM HEPES pH7.9, 10 mM KCl, 0.1% Triton X-100, 20% glycerol, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 10 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Microbiology 2024Quote: Cell harvesting and disruption were performed with the multi shot cell disruptor at 20,000 psi using buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Protease inhibitor (Roche, Basel, Switzerland) for cell resuspension and disruption ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in lysis buffer (100 mM HEPES pH 7.5, 200 mM NaCl, 10% glycerol) supplemented with EDTA-free Protease inhibitor cocktail (Roche, Cat#05056489001), 1 mM phenylmethylsulfonylfluoride (Sigma ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 100 mg of tobacco leaf tissue using the TriPure reagent (Roche Sigma-Aldrich, St. Louis, MO), according to the manufacturer’s instructions ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Cells were scrapped into 100 µl of RIPA lysis buffer (Astral Scientific 786-490) supplemented with protease inhibitor cocktail (Roche 11697498001) and transferred to microfuge tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
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bioRxiv - Developmental Biology 2024Quote: ... The discs were dissected in pre-cooled PBS and pipetted in PBT (PBS, 0.1 % Triton X-100) containing 1x proteases inhibitors (cOmplete™ Protease Inhibitor Cocktail tablets, Roche, 11873580001). The disks were incubated in PBT with 1x protease inhibitors and 0.4 % IGEPAL CO-630 (Sigma ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by incubating cells in RIPA lysis buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP40, 1% deoxycholate, 0.1% SDS, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF) on ice for 30 minutes and cell lysate was clarified by centrifugation at high speed (15 000 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... 1% NP-40, 150 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0 and Roche complete protease inhibitor EDTA-free) and acid-washed glass beads using a Precellys machine (6 × 30 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently lysed (1 mM NaF, 1 mM Na3VO4, 10 nM calyculin A, 1 mM PMSF, 1 mg complete EDTA-free protease inhibitor cocktail [Roche] in RIPA buffer), scraped ...
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bioRxiv - Physiology 2022Quote: ... cell pellets were resuspended in RIPA Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% IGEPAL CA-630, 1% deoxycholate, 1 mM DTT, DNase, and Roche cOmplete Protease Inhibitor Cocktail) and lysed via sonicating water bath ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested in HBSS medium containing 1.07 Wünsch units/ml of Liberase DH (Roche) and 600 IU of Hyaluronidase (Calbiochem ...
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bioRxiv - Cell Biology 2020Quote: ... the bile duct was cannulated and perfused with 2◻mL collagenase P (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: Embryos were dissociated in 0.9x ice-cold PBS using 0.5mg per mL Liberase (Roche) then resuspended in 0.9x PBS/1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... Magnetic beads were removed by magnet and 1mg/ml of anti-HA antibody (Roche) was added to the proteins for incubation overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Lung was inflated with a digestion solution containing 1.5mg/ml of Collagenase A (Roche) and 0.4mg/ml DNaseI (Roche ...
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bioRxiv - Immunology 2021Quote: ... placed in DMEM with 30 µg/mL Liberase TM Research Grade (Roche Applied Science) and incubated at 37°C for 90 min (shaking).
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bioRxiv - Developmental Biology 2019Quote: ... 500,000 cells from limb buds were then dissociated with 100ug/mL Liberase (Roche 05401119001), resuspended in CSKT buffer (10mM PIPES pH6.8 ...
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bioRxiv - Immunology 2020Quote: ... The cells were plated in pMCPECs media on 50ug/ml laminin (Roche, Mannheim, Germany) coated inverted filters of 5μm pore size and 6mm diameter (Corning ...
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bioRxiv - Pathology 2019Quote: ... and 200 μL of 20 mg/mL Proteinase K (Roche FFPET Isolation Kit # 06650775001) was added to each tube ...
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bioRxiv - Developmental Biology 2019Quote: ... Deparaffinized and rehydrated sections were treated with 8 μg/mL of Proteinase K (Roche) in PBS for 10 min ...
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bioRxiv - Genomics 2019Quote: ... Sections were placed in 4,6-diamidino-2-phenylindole (DAPI) (0.2 g /ml; 236276; Roche) for 10 minutes and then mounted on plus coated slides and coverslipped using Vectashield (H-1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with rat anti-HA antibodies (Roche, 3F10; 0.2 μg/ml) for 1 hr ...
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bioRxiv - Genetics 2021Quote: ... followed by addition of 5 µl of 4 µg/mL DNase-free RNase (Roche). Samples were loaded onto 96-well Qiacube-HT® columns and DNA was purified using a Blood & Tissue kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... the tissue was minced finely and digested with 0.28 units/ml Liberase TM (Roche) and 0.1 mg/ml of Deoxyribonuclease I (Worthington ...
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bioRxiv - Biochemistry 2021Quote: ... Complete EDTA-free protease inhibitor cocktail (one tablet per 50 mL of buffer, Roche) and 0.1 mg/mL DNaseI) ...
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bioRxiv - Biochemistry 2021Quote: ... Complete EDTA- free protease inhibitor cocktail (one tablet per 50 mL of buffer, Roche)) – 50 mL lysis buffer per litre culture – and incubated on ice for 30 min followed by sonication ...
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bioRxiv - Pathology 2021Quote: ... Obex samples had 4 µl of 1000 µg/mL of proteinase K (PK, Roche) (diluted in 1x PBS and 0.5 M EDTA ...
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bioRxiv - Cancer Biology 2020Quote: ... established KP tumorspheres were mechanically and enzymatically dissociated with 2µg/ml collagenase/Dispase (Roche). To obtain single-cell suspensions ...
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bioRxiv - Cell Biology 2021Quote: ... and mEpiSCs and MEFs were cultured on 10 µg/mL/cm2 fibronectin (Roche 11051407001). Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were isolated from mouse brains with Collagenase D (0,5 mg/ml, #11088866001, Roche), DNase I (10μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with a 1µg/ml solution of the DNA-dye DAPI (Roche) and mounted with the Slowfade antifade reagent (Invitrogen) ...
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bioRxiv - Systems Biology 2020Quote: ... and enzymatically digested with deoxyribonuclease type I (0.1 mg/mL; Roche, Burgess Hill, UK) and collagenase (0.5 mg/mL ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... except for HSPGs where cells were pretreated or not with pronase (3,5mg/mL, Roche) and then subjected to immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA, mouse monoclonal primary antibody (12CA5 Roche, 5 mg/ml) at a dilution of 1:1000 ...