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Citations for Roche :
4351 - 4400 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of pcDNA 3.1(-) empty vector or C/EBPβ deletion mutants in 100 mm dishes using X-tremeGENE HP (Roche) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then rinsed in methanol followed by PTX (0.3% Triton X-100 in PBS 1x) and incubated in blocking reagent (Roche) for 1 hour at RT ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl) plus 0.5% Triton X-100 and one tablet of cOmplete ™ Protease Inhibitor Cocktail Tablet (Roche).
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bioRxiv - Genomics 2020Quote: ... and resuspended in 1 mL Nuclear Isolation Buffer (20mM HEPES, 10mM KCl, 1mM MgCl2, 0.1% Triton X-100, 20% Glycerol, and 1X Protease Inhibitor (Roche)) per 5-10 e6 cells by gently pipetting 5x with a wide-bore tip to release nuclei ...
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bioRxiv - Biophysics 2020Quote: ... RNC samples were buffer exchanged into deuterated Tico buffer (100% D2O and d8-HEPES, pH 7.5) supplemented with protease inhibitors (Roche), with a final sample concentration of 7.5 μM ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen retinas were subsequently homogenized by repetitive pipetting on ice in PBS with 0.5% TritonX-100 and cOmplete protease inhibitors (Roche). The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Whole brain samples were homogenized in carbonate buffer (pH 11.5, 100 mM Na2CO3, 50 mM NaCl, protease inhibitor) using the MagNA Lyser (Roche) for 5 s at 5000 speed ...
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bioRxiv - Biochemistry 2019Quote: ... nuclei were harvested as above and the nuclear pellet resuspended in 100 µl extraction buffer (3mM EDTA, 0.2mM EGTA and protease inhibitors (Roche)) ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% NP-40, 0.5% TX-100, 150 mM NaCl plus complete EDTA-free Protease Inhibitor Cocktail Tablets, Roche, #11697498001), diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... is followed by probe titration (100-300 ng per slide dependent on the probe, dissolved in Ribohybe reagent (Roche)) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was fragmented to 100-300bp and libraries were constructed using the KAPA HTP Library Kits (KAPA Biosystems). A single low input sample used the Swift Accel-NGS 2S DNA Library Kit (Swift BioSciences ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in 50–100 mL Strep-tag binding buffer (100 mM Tris-HCl, 150 mM NaCl, pH 8.0, sterile filtrated) supplemented with one protease inhibitor tablet (Roche), 0.1 % of Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... and lysed in 1 mL of 1% Triton X-100 lysis buffer (50mM Tris-HCl [pH 7.4], 150mM NaCl) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 20 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... cell pellets were lysed in 4% SDS/100 mM Tris HCl pH 7.4 supplemented with cOmplete protease inhibitor cocktail (Roche), and sonicated at 4 °C using a Diagenode Bioruptor ...
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bioRxiv - Genomics 2019Quote: ... 12000g and supernatant diluted with 1 volume of Dilution Buffer (1mM EGTA 300 mM NaCl, 2% Triton x-100, 0.2% sodium deoxycholate, 1x protease inhibitors-Roche). Sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 6 ug of antibody conjugated to 20 µl of Dynabeads ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then washed 2 × with washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, 2 mM EGTA, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 mM Tris-HCl pH 7.5 (Quality Biological, USA, #351-006-101) and protease inhibitors (Roche, Germany, #118636170001) and sonicated using a Sonic Dismembrator (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were prepared from 100 ng of total RNA using RNA HyerPrep kit with RiboErase (Kapa Biosystems). Sequencing was performed at the MD Anderson core facility equipped with an Illumina HiSeq 4000 instrument ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were re-suspended in lysis buffer (phosphate buffered saline containing 0.5% Triton-X 100 and complete protease inhibitors [Roche]) and DAPI was added (final concentration 5μM ...
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bioRxiv - Genomics 2021Quote: ... Pre-capture libraries were prepared from up to 100 ng input genomic DNA using the Kapa Hyperplus kit (Roche), using a fragmentation time of 8 minutes and standard-chemistry end repair/A-tailing ...
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bioRxiv - Neuroscience 2021Quote: ... PFA was removed and cells were washed 3x with PBS then blocked in a buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 100 µL Lysis Buffer (10 mM Tris-HCl pH 7.5, 10 mM EDTA, protease cocktail inhibitor (Roche), 0.1 mg/mL lysozyme ...
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bioRxiv - Neuroscience 2020Quote: Whole zebrafish brains were placed in 100 µL of 1X Complete Protease Inhibitor Cocktail (Roche Holding AG, Basel, Switzerland) in RIPA buffer (Sigma-Aldrich Corp ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Developmental Biology 2022Quote: ... A small amount of each organ was cut and homogenized in ice-cold lysis buffer containing 0.3% Triton X-100 in PBS with protease inhibitor (complete mini EDTA free protease inhibitor, Roche). The suspension was centrifuged for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were washed with 200 μl of solution A (with 10 mL of Tris-HCl at 100 mM pH 7.4 and one cOmplete Mini EDTA-free tablet of protease inhibitor cocktail from Roche) and pelleted with benchtop centrifuge ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections from proximity labeling experiments were lysed and de-crosslinked in 100 mM Tris-HCl buffer (pH = 8.0) with 2% SDS and protease inhibitor (Roche) at 95 °C for 45 min with constant shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche]). Cell lysis was obtained by a mechanical-pestle and sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... containing Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). After centrifugation ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
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bioRxiv - Plant Biology 2024Quote: ... 100 nM of each forward and reverse primer (Supplementary Table S4) and 125lllng λ-DNA (Roche Diagnostics, Vilvoorde, Belgium) was mixed with 2lllμL of a 12x diluted sample cDNA ...
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bioRxiv - Microbiology 2023Quote: ... samples were collected and washed twice in 1x phosphate buffered saline (PBS) and resuspended in 100 mM Tris-HCl (pH 8.5) containing a protease inhibitor cocktail tablet (Roche). Following the addition of sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM MOPS) pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany) on ice ...
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bioRxiv - Immunology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cortical tissue was suspended in 600μl lysis buffer (50mM Tris-HCl, 140mM NaCl, 10% Glycerol, 0.5% IGEPAL, 0.25% Triton X-100, protease inhibitor cocktail (Roche, 4693124001)) and manually homogenized ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellet was resuspended in a lysis buffer of Buffer A + 0.5% Triton X-100 containing 5% protease inhibitor cocktail cOmplete (Roche, 118614500 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C for 5 min in SDS lysis buffer (5% SDS in 100 mM triethylammonium bicarbonate pH 8.5, complete EDTA free protease inhibitor cocktail (Roche), phosphatase inhibitor cokatail-2 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 ng/μL for the rest of the genes) in hybridization buffer [100 μg/μL RNA from yeast (Roche) added to pre-hybridization buffer] at 60°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... were equilibrated with dilution buffer (20 mM Tris, 150 mM NaCl, 0.5% Triton X-100 and protease inhibitor cocktail (Roche), pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in PBS supplemented with 0.5% (v/v) Triton X-100 and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) (PI) ...
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bioRxiv - Neuroscience 2023Quote: ... chopped samples were transferred to a 15 ml Dounce Homogenizer with 5ml homogenisation buffer (100 μM of DTT [Sigma–Aldrich, St. Louis, MO, USA], 0.1% Triton X-100 [Sigma–Aldrich], 2X EDTA Protease Inhibitor [Roche, Basel ...
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bioRxiv - Molecular Biology 2024Quote: ... with Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). Proteins were isolated from the supernatant ...
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bioRxiv - Physiology 2024Quote: ... 100 nanograms of total RNA were used for library preparation with the KAPA total RNA Hyperprep Kit (KK8581) (Roche). Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed from approximately 100 ng of genomic DNA using the Kappa HyperPrep Kit (Roche, Pleasanton, CA, USA) with mechanical shearing (Covaris ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in 1 mL of 1% Triton X-100 lysis buffer (50mM Tris-HCl [pH 7.4], 150mM NaCl) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 20 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... pre-capture libraries were prepared from up to 100 ng of input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...