Labshake search
Citations for Roche :
4001 - 4050 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 25 wt% sucrose and 2 mg/mL lysozyme (from egg white, 10837059001 Roche, Basel, Switzerland) and incubated for 30 min at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated with 10 mL of anti-GFP primary antibodies (Roche, Cat.#11814460001) at a concentration of 1 µg/mL in 4 % milk/TBS-T for 1 h ...
-
bioRxiv - Immunology 2024Quote: Liver single cell suspensions were prepared after digestion with liberase TL (0.8 U/ml; Roche) and DNase (160 U/ml ...
-
bioRxiv - Immunology 2024Quote: ... The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml; Roche, Switzerland) for 20 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) containing phosphatase inhibitors (10 mM sodium pyrophosphate, 20 mM β-glycerophosphate, 100 mM NaF) and protease inhibitor cocktail (Roche Applied Science). Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz ...
-
Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-Buffered Saline + 1% Triton X-100 (TBST) with protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
-
bioRxiv - Physiology 2021Quote: 50 islets per sample were washed in PBS twice and then lysed and sonicated in RIPA buffer (10mM HEPES, 50mM β-glycerol phosphate, 1% Triton X-100) supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC) and phosphatase inhibitors (2mM EGTA ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5% SDS, 0.5% SDO, 1% Triton X-100, 1 mM PMSF, Roche cOmplete Mini Protease Inhibitors 1x), and homogenized (Precellys Evolution ...
-
bioRxiv - Neuroscience 2022Quote: ... incubated with high salt HKT buffer (25mM HEPES pH 7.2, 1M NaCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 10 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were collected and incubated in HKT lysis buffer (25mM HEPES pH 7.2, 150mM KCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 30 minutes on ice prior to homogenization with a 25-gauge syringe needle ...
-
bioRxiv - Microbiology 2021Quote: ... The pellet was suspended in 100 μL of staining solution containing FITC-conjugated Annex-in-V and propidium iodide (Annexin-V-Fluos Staining Kit, Roche Molecular Biochemicals, Germany) and incubated for 15 min at 20 °C ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed with cold PBS and resuspended in NE1 buffer (20 mM HEPES-KOH pH7.9, 10 mM KCl, 1mM MgCl2, 1 mM DTT, 0.1% triton X-100 supplemented with Roche complete protease inhibitor EDTA-free) and rotated for 10 minutes at 4°C ...
-
bioRxiv - Physiology 2020Quote: Tissues were flash frozen after harvest and homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
-
bioRxiv - Developmental Biology 2019Quote: ... ESCs cells and neurospheres were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete™ Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
-
bioRxiv - Clinical Trials 2019Quote: ... participants were given orange juice (with or without 100/25 mg of L-dopa/benserazide, trade name: Madopark® Quick mite. Roche, Basel, Switzerland) by a nurse who was masked to the group specification ...
-
bioRxiv - Physiology 2021Quote: Starting with frozen tissue, tissue was homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
-
bioRxiv - Physiology 2021Quote: Tissues were flash frozen after harvest and homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT, 150 mM NaCl, 5 mM MgCl2, 0.001% Triton X-100, 1mM PMSF, 0.2 mM GDP, Roche cOmplete EDTA-free protease inhibitors) and lysed with a microfluidizer ...
-
bioRxiv - Genetics 2019Quote: ... Sheered chromatin samples were pre-cleared by incubating with the 250ul aliquot of Dynabeads resuspended in 2ml of Dilution Buffer (20mM Tris pH=8, 2mM EDTA, 150mM NaCl, 1% Triton X-100, 1:50 Roche cOmplete protease inhibitor) for 1 hour at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete, Roche Diagnostics, Mannheim, Germany). Protein concentrations were determined by BCA-assay (Thermo Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... and then mixed with 4ml protein extraction buffer (50mM Tris/HCl pH 7.5, 5% glycerol, 150mM NaCl, 0.2% triton X-100, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) as soon as the ground tissue had reached room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... The target loci of interest were PCR-amplified from 100 ng genomic DNA using KAPA HiFi HotStart Uracil+ Ready Mix (Kapa Biosystems, Cat # KK2602) or Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1% Triton X-100, 1 mM DTT, 1 mM PMSF, 1× Roche protease inhibitor cocktail) and about 500 μL of 0.5-mm-diameter glass beads ...
-
bioRxiv - Immunology 2023Quote: Total hemolymph was harvested by bleeding ten L3 wandering larvae in 100 μL of Phosphate Buffer Saline (PBS) supplemented with Complete Inhibitor (Roche, diluted 1/50), PMSF (1 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.2% Triton X-100, 1 mM EDTA, 1 mM dithiothreitol, 1 mM NaF, 10 mM β-glycerophosphate, 0.1 mM Na3VO4, 1x Roche cOmplete protease inhibitors). Protein lysates (12 µg per lane ...
-
bioRxiv - Cell Biology 2022Quote: ... lung homogenates and EV-Pellets were lysed in Triton X-100 lysis buffer supplemented with protease and phosphatase inhibitors (Roche Diagnostics, Mannheim, Germany) and protein concentrations were quantified using a modified Lowry assay (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 mM Na-β-Glycerophosphate and 1% (v/v) TritonX-100 and immediately prior to use the buffer was supplemented with cOmplete™ protease inhibitor cocktail (Roche. Cat# 04693116001).
-
bioRxiv - Cell Biology 2023Quote: ... Protein expression was determined by solubilizing 2–4 x 106 cells in lysis buffer (1% Triton X-100, 150 mM NaCl, 0.5 mM EDTA) plus 2X protease inhibitor cocktail (Roche Applied Science, Boulder, CO). Samples were analyzed by SDS-PAGE and Western analysis was performed using either mouse anti-FLAG antibody (1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100, and cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM PMSF and 1 tablet/80 ml of complete EDTA-free protease inhibitor cocktail (Roche)] ...
-
bioRxiv - Cell Biology 2020Quote: The PDMS micro-wells were UV sterilized and coated with fibronectin (50 µg/mL) (Roche Lifescience). For cell seeding ...
-
bioRxiv - Microbiology 2019Quote: ... re-suspended in cold phosphate-buffered saline (25 mL) containing a protease-inhibitor cocktail (Complete, Roche), and lysed by two passages through a French pressure cell at 1,500 psi ...
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were re-suspended in PBS containing 50 μg ml-1 RNase A (Roche Diagnostics) for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The sample was incubated for 2 h with 2 μl of 20 mg/ml ProteinasK (Roche), and subjected to AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 30 min at 37°C and additionally with 1 mg/ml of collagenase solution (Roche) for 12-18 hr at 37°C with agitation ...
-
bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed in PBS and the nuclei were counterstained with DAPI(1 μg/ml, Roche Diagnostics ...
-
bioRxiv - Cell Biology 2022Quote: ... Brain tissue was minced with scissors and incubated in 4,000 U/ml of collagenase D (Roche) at 37°C for 30 minutes ...
-
bioRxiv - Genomics 2019Quote: ... The remaining fragments were then treated with a collagenase/dispase mixture (2 mg/mL final) (Roche) and DNase I (2 mg/mL final ...
-
bioRxiv - Immunology 2021Quote: Kidneys were chopped up and incubated in a digestion medium containing 0.2mg/ml Liberase TL (Roche) and 0.1mg/ml DNase I (Roche ...
-
bioRxiv - Immunology 2019Quote: ... skin samples were digested for 1h at 37°C using 1 U/mL Liberase TM (Roche) and 5 µg/mL DNAse I (Sigma) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 mM NA3VO4 and 1 μg/ml leupeptin supplemented with a complete mini protease inhibitor (Roche). Proteins (30–40 μg ...
-
bioRxiv - Biophysics 2019Quote: ... and one tablet of Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4) with up to seven strokes at 700 rpm in a Teflon glass homogenizer ...
-
bioRxiv - Cancer Biology 2019Quote: ... mechanical disrupted and incubated in RPMI medium containing 1mg/ml of DNase I (Roche, cat 10104159001), 200U of collagenase IV (Gibco ...
-
bioRxiv - Microbiology 2019Quote: ... and adjusted to 1 x 108 tachyzoites/ml with PBS containing protease inhibitors (cOmplete mini, Roche). Parasites were lysed by five freeze-thaw cycles (liquid nitrogen/37°C) ...
-
bioRxiv - Developmental Biology 2019Quote: ... To increase probe permeability embryos were incubated at 21°C in 10µg/ml proteinase K (Roche) for the following durations ...
-
bioRxiv - Molecular Biology 2019Quote: ... Fixed ovaries were lysed in 1 ml of RIPA Buffer (supplemented with complete protease inhibitors (Roche) and RNasin Plus 40U/ml ...
-
bioRxiv - Cell Biology 2019Quote: ... left panel) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 25 ml, Roche, Mannheim ...
-
bioRxiv - Immunology 2019Quote: ... Lungs were finely minced and digested in complete RPMI supplemented with collagenase D (0.1mg/mL; Roche) and DNase I (20U/mL ...
-
bioRxiv - Cell Biology 2020Quote: ... HA-Kir6.2 was detected with HRP-conjugated rat anti-HA antibody (Roche, 3F10, 0.005 μg/ml). GFP was detected with mouse anti-GFP antibodies (AbCam ...