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Citations for Roche :
3851 - 3900 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... tubes and homogenized for 1.5 minutes in 50μL of TBS containing 1% Triton X-100 (Table 1) and 1X protease and 1X phosphatase inhibitor cocktails (Roche, 04693124001 and 04906837001). The homogenates were then probe-sonicated with a Branson Sonifier® SFX150 (Emerson ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Cell Biology 2024Quote: ... or 48 h after seeding in a Triton X-100-based buffer (150 mM NaCl, 20 mM Tris-HCl pH 7.5, 5 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). A linear sucrose density gradient was prepared in 13x51 mm centrifuge tubes (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1mM EGTA, 1% Triton X-100, 0.4% SDS, 1% NP-40, 1× Roche protease inhibitor cocktail), one time with wash buffer B (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail [Roche-11697498001], 1 mM PMSF), incubated on ice for 10 minutes and centrifuged for 10 minutes at 21100 x g at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Genomics 2023Quote: ... Cells were resuspended in ½ volume ice-cold NE1 buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5mM spermidine, 0.1% Triton-X-100, 20% glycerol with Roche Complete Protease Inhibitor EDTA-free) and lysed on ice for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated overnight at 60°C in 100 μl of hybe-solution buffer containing 20 ng digoxigenin (Dig)-labeled probes (see below) for colorimetric detection (Roche, cat. #11277073910) or 20 μg of fluorescein-labeled probes (see below ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 0.1 mM EGTA, 100 mM NaCl, 1% Nonidet P-40, 10 mM Imidazole, supplemented with Roche protease inhibitor cocktail). Cell lysates were clarified by centrifugation at 10,000× g for 5 min at 4 °C and then incubated at 4 °C under constant rotation with His-ΔFIP1c-RCP559-649 fusion protein ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA, 1% SDS, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride, and 1× Roche Protease Inhibitor Cocktail) and sonicated to reduce the average DNA fragment size to ∼500 bp ...
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bioRxiv - Microbiology 2023Quote: ... Purified PARP10 was diluted to 20 µM using MARylation buffer (SEC buffer supplemented with 0.02% Triton X-100 and 200 µM β-NAD+ (Roche, NAD100-RO)) ...
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bioRxiv - Neuroscience 2023Quote: Lysates from cultured neurons were collected in NP40 lysis buffer (150 mM NaCl, 100 mM Tris-HCl, 1% NP40 in PBS) containing 1X protease inhibitors (cOmplete ULTRA tablets, Roche, cat# 05892970001). Samples were run on mini-protean TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were collected and lysed in lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Diagnostics, Mannheim, Germany]) at 4°C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Developmental Biology 2024Quote: ... the extirpated tongues from non- transgenic and Lgr5-EGFP-IRES-CreERT2;R26-mTmG reporter mice were injected with approximately 100-200 μl 1% Dispase II (neutral protease; Roche, Indianapolis, IN) dissolved in Balanced Salt Solution (BSS ...
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bioRxiv - Microbiology 2024Quote: ... Samples were lysed in 1% Triton X-100 (20 mM Tris [pH 7], 150 mM NaCl, 5 mM MgCl2) containing protease inhibitor (Roche, Cat# 11697498001) with end-over-end rotation for 20 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 5% Sarkosyl) and samples were treated with proteinase K (50μg/mL) and Ribonuclease A (Roche) for 30 minutes at 37°C to remove contaminating proteins and RNA ...
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bioRxiv - Microbiology 2020Quote: Fresh cultures were inoculated from glycerol stocks into 400μl 7H9 with kanamycin (Roche, 20ug/ml) and grown until saturated ...
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bioRxiv - Microbiology 2020Quote: ... Exponential phase cultures were inoculated 1:40 into 7H9 supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma 100ng/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed in 2X PIC (Roche mini-tabs, 1 tab in 5 ml = 2X) and stored at -80°C until needed.
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bioRxiv - Molecular Biology 2020Quote: For sequencing all samples were treated with 4 mg/ml of proteinase K (Roche Diagnostics). RNA and small RNA were extracted and enriched in separate fraction from the supernatants using the mirVana kit according to manufacturer’s instructions to separate samples into two groups ...
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bioRxiv - Neuroscience 2021Quote: ... 0.3mg or 1mg/ml/kg midazolam (Hypnovel, Roche, diluted with 0/9% w/v saline) fifteen minutes prior to a standard punishment session ...
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bioRxiv - Molecular Biology 2021Quote: ... The tissue was minced and transferred to Enzyme cocktail [0.25 mg/mL Liberase TH (Roche), 20 U/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with labeled probe and 250 µg/mL salmon sperm DNA (Roche) at 65°C overnight in Perfecthyb Plus Hybridization Buffer (Millipore) ...
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bioRxiv - Immunology 2019Quote: ... The lungs were removed and infiltrated with 2 mg/ml collagenase D (Roche, Indianapolis, Indiana) and 0.2 mg/ml DNase I (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM DTT) containing Complete™ Protease Inhibitor Cocktail (one tablet per 50 mL) (Roche) and disrupted by sonication (Branson Digital Sonifier ...
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bioRxiv - Cell Biology 2019Quote: ... Two ml of ice-cold lysis buffer supplemented with protease inhibitors (Roche Applied Science, 11836145001), phosphatase inhibitors (Calbiochem ...
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bioRxiv - Genetics 2021Quote: ... Pellets were thawed on ice and 750 ml of FA buffer containing protease inhibitors (Roche Cat#11697498001 Complete Protease Inhibitor Cocktail Tablet ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 30 μl of 20 mg/ml proteinase K (Roche 03 115 828 001). The samples were incubated overnight at 55 °C ...
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bioRxiv - Immunology 2021Quote: ... Then fragments were digested in RPMI 1640 medium containing Liberase™ (Roche, 0.05 U/mL) plus DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with one EDTA-free protease inhibitor mini tablet / 5 ml of buffer (Roche #4693159001). Lysates were incubated on ice for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/mL DNAse and one complete mini EDTA-free protease inhibitor cocktail tablet (Roche), by passing the sample three times through a pressurised cell disruptor (M110-L ...
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bioRxiv - Bioengineering 2020Quote: ... antigens were retrieved using 1 mg/ml pronase/PBS (11459643001; Roche life science, Indiana, USA) and 10 mg/ml hyaluronidase/PBS (H2126 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The heads or brains were then digested with 10ug/mL Proteinase K (Roche, RPROTK-RO) for 10-20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then digested by addition of 2 μL 20 mg/mL Proteinase K (Roche) and incubation for 2 h at 55° C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was removed to 2 ml Eppendorf tubes and incubated with HA antibodies (Roche) for 30 min on a rotator at 4° ...
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bioRxiv - Systems Biology 2021Quote: ... epidermal sheets were digested in Liberase Tm (13 U/ml, Roche, UK, 2h, +37°C,) and enriched using density gradient centrifugation (Optiprep 1:4.2 ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μL of the supernatant were incubated with 20 μg/mL proteinase K (PK; Roche) in a total volume of 22 μL RIPA buffer for 1 hr at 37°C to digest all proteins except for PK-resistant PrPSc ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μg/ml leupeptin) containing CompleteTM Mini Protease Inhibitor Cocktail (Roche Diagnostics, Mannheim, Germany) by sonication ...
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bioRxiv - Neuroscience 2022Quote: ... Minced pieces are then subjected to enzymatic dissociation in collagenase/dispase (Roche, 100mg/ml stock) solution for 30 min at 37°C in a hybridization oven with constant rotation ...
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bioRxiv - Biochemistry 2022Quote: ... containing 150 mM sodium chloride and 1 mL of 50 x Complete Proteinase inhibitor (Roche 45582400 ...
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bioRxiv - Cell Biology 2022Quote: ... postnatal and adult hearts were minced and digested in 2.5 mg/mL collagenase D (Roche), 0.25 mg/mL DNase I (Roche ...