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Citations for Roche :
3801 - 3850 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Minced adipose tissue was incubated with 0.28 U/ml of liberase TM (Roche #05401119001) in DPBS with calcium and magnesium (Gibco #14040091 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 ml per 2.5×107 cells) supplemented by EDTA-free complete protease inhibitor (Roche). Lysate was prepared by three cycles of freeze-thawing ...
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bioRxiv - Microbiology 2023Quote: ... filtered through 0.45 μm filter and treated with 10 U/ml DNase I (Roche) for 30 min at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... and 0.165 mg/mL BCIP (5-Bromo-4-chloro-3-indolyl phosphate, Roche, 11383221001) in alkaline phosphatase buffer ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was treated with 2 mg/mL of pronase from Streptomyces griseus (Roche) in 50 mM Tris-HCl pH 7.0 for 1.5 h at 60°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumors were digested by incubation in liberase TM (0.05 mg/ml; Roche, #05401119001)+DNAse (0.2 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: Lungs were digested by instilling 0.8 mg/ml collagenase/dispase (#10269638001 Roche, Indianapolis, IN) and 2 mg/ml pronase (#10165921001 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mg/mL DNaseI (Gold Biochem) and a single “Complete” protease inhibitor tablet (Roche). Cells were lysed by sonication and the lysate clarified by centrifugation and applied to glutathione agarose (1 mL bed volume ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested with digestion buffer (Gibco DMEM/F12, Fisher 11320033; 4 µg/ml Roche Collagenase/Dispase ...
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bioRxiv - Neuroscience 2023Quote: ... 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and protease inhibitor (1 pill per 10 ml; cOmplete Mini EDTA-free, Roche Diagnostics). The lysates were sonicated and then centrifuged at 14,000 x g for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... then 500 μL of a dissociation solution containing 0.1 mg/mL Liberase TM (Roche) and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.8mM CaCl2) supplemented with 250uL of 5mg/mL Liberase (Roche, 05 401 127 001) and incubated for 2h30-3h at room temperature (RT ...
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bioRxiv - Physiology 2024Quote: ... The final pellets were suspended in 200 μL of 0.3 mg/ml amyloglucosidase (Roche) in 0.2 M sodium acetate (pH 4.8) ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed in L-15 medium with 40 μg/ml DNaseI (Roche) and transfected with 2 μg plasmid DNA using the 4D- Nucleofector (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... the cells (108/ml) were pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM ...
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from Lonza and cultured in coated flasks with 50 μg/ml rat tail collagen I (Roche), with Vasculife (LL-0003 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently adjusted 105 cells/ml cell densities using a CASY cell counter (Roche). Three µl of fungal cell suspensions were applied topically to the center of the biopsies ...
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bioRxiv - Biochemistry 2024Quote: We diced lung tissue samples and digested them with 0.25 mg/ml Liberase (Roche) and 1 mg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The tissues were then digested with 50 μg/mL DNase I (Roche, Cat#: 10104159001) and 300[U/mL collagenase VIII (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos were treated with 10 μg/mL proteinase K (Roche, catalog no. 3115887001) in PBST for a duration of 10 to 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... containing 0.5% BSA and 3 mg/mL DNAse I grade II (Roche, Cat# 104159). Once resuspended ...
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bioRxiv - Biophysics 2024Quote: ... 70 μg/ml lysozyme and cOmplete mini EDTA-free protease inhibitor cocktail (Roche, 11836170001)] per liter of cultured E ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole cell lysates were extracted from trans transfected 293T cells or infected LNCaP cells or PC-3 cells by IP lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). An aliquot of the cell lysate was kept as input for western blot analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first thawed on ice and resuspended in 1 mL FA buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, with Roche cOmplete protease inhibitors (Sigma)) ...
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bioRxiv - Genomics 2020Quote: ... a total of 100 ng DNA from each bacterial strain was used according to KAPA Hyper Prep kit (KAPA Biosystems, MA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) ...
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bioRxiv - Genetics 2019Quote: ... of each library was used for bar-coding amplification using dual-barcoded single-stranded library adapters44 as primers in the following 100 μl volume reaction: 10 μl 10x PCR Buffer + MgCl2 (Roche, Basel, Switzerland) 0.4 μM of each primer ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were harvested with 500 µl RIPA-buffer (Tris-HCl, NaCl, Triton X-100, sodium deoxycholate, SDS, EDTA, pH 7.4) with Complete protease inhibitor (Roche Diagnostics, Basel, Switzerland) and protein concentration was measured using a Bradford assay (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... vortexed in 200 µL ice-cold extraction buffer (20 mM Tris pH 7.5, 300 mM NaCl, 5 mM MgCl2, 0.1% Triton X-100, 1x complete Proteinase-Inhibitor Cocktail from Roche, 50 µM MG-132) and incubated for ten minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... the resulting cell pellets were re-suspended in 500 uL of lysis buffer (1X PBS, 0,5% Triton X-100, cOmplete EDTA-free protease inhibitor cocktail, Roche Diagnostics, Basel, Switzerland) and incubated for 10 min on ice ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were harvested by centrifugation, resuspended in lysis buffer (20 mM Tris, 100 mM NaCl, 20 mM imidazol, protease inhibitor (Roche, Mannheim, Germany)) and lysed by 3-4 passages through Emulsiflex B15 (Avestin ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in French Press buffer (40 mM HEPES, 100 mM KCl, 20 mM imidazole, 2 mM PMSF, EDTA-free cOmplete protease inhibitor [Roche], pH 7.5) for 1 hour followed by homogenization using tight douncer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
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bioRxiv - Immunology 2019Quote: ... and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS, 1% Triton X-100, 2mM EDTA, 50mM Hepes-KOH [pH 7.5], 0.1% sodium deoxycholate, Roche 1X Complete Protease Inhibitor). Nuclei were lysed for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100, and 1 mM EDTA) containing a phosphatase inhibitor cocktail (Nacalai Tesque, Kyoto, Japan) and protease inhibitor cocktail (Roche, Basel, Switzerland). After centrifugation at 20,380×g for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 5 mM imidazole, 0.4% Triton X-100, supplemented with Roche cOmplete Protease Inhibitor Cocktail) and lysed by sonication ...
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bioRxiv - Biochemistry 2021Quote: ... washed twice with PBS and subsequently resuspended in assay buffer (20 mM HEPES pH 7.5, 150 mM NaCl, and 0.1% Triton X-100 supplemented with Roche cOmplete Protease Inhibitor Cocktail). The cells were lysed by sonication and cleared by centrifugation for 5 min at 14’000 rpm and 4°C ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: Frozen yeast lysate powder was resuspended in ice-cold lysis buffer (50M Tris-HCl, pH 7.4, 150mM NaCl, 0.05% Triton X-100, 1x Roche cOmplete Proteinase inhibitor cocktail) to a concentration of 6µg/µl and rotated for 10 min at 4°C before centrifugation at 3000 x g for 10min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl2, 1% Triton X-100, 10% Glycerol, 1 mM DTT, Roche complete protease inhibitor) with 0.5 mg/mL Lysozyme and placed on a roller for 45 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...