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Citations for Roche :
4201 - 4250 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in Tris-Buffered Saline (TBS) + 1% Triton X-100 with EDTA and protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Biochemistry 2024Quote: ... 250 mg of powder was thawed at 4°C followed by resuspension in 1 mL of HIP buffer (40 mM HEPES-KOH pH 7.5, 110 mM KOAc, 2 mM MgCl2, 1% Triton X-100, 0.1% Tween, 1x protease inhibitor cocktail [Roche], 1% solution P, 1 mM DTT). The lysate was next passed through a Whatman 25 mm GD/X Disposable filter (Cat No ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Plant Biology 2022Quote: ... 1/100 cDNA dilutions were analysed using a LightCycler®480 and LightCycler®480 SYBR Green I Master (Roche, Welwyn Garden City, UK) following manufacturer’s instructions and cycling conditions of pre-incubation 95°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... resuspended in 150 μL buffer C (50 mM Tris pH 8, 5 mM EDTA, 1% SDS, 100 mM NaCl, 1x Roche complete mini protease inhibitors) and incubated on ice for 10 min ...
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bioRxiv - Physiology 2024Quote: Total protein was extracted from whole brain samples by homogenizing in 100 µL of 1X RIPA buffer containing protease inhibitors (cOmplete Mini Protease Inhibitor Cocktail, Hoffmann La Roche Limited, Mississauga, ON, Canada) and prepared for Western blotting exactly as previously described (Dindia et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 300 mM NaCl, 30 mM imidazole, 1 M urea, 1% v/v Triton X-100, 5 mM beta-mercaptoethanol, with Roche EDTA-free cOmplete protease inhibitor) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using the extracted DNA (100 ng) as template with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was discarded and the pellet was resuspended in a 1:1 solution of 100 mM Tris-HCl pH 7.4 with EDTA-free protease inhibitor cocktail (Roche, 1 tablet in 10ml of buffer) and 4x NuPage LDS sample buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% Triton X-100, 10 mM N-methyl maleimide (general DUB inhibitor diluted in DMSO, freshly added) and protease inhibitors (Roche Diagnostics, EDTA-free, freshly added). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Cultured cells were harvested at different time points as indicated in the figures and lysed in 1% Triton X-100 buffer containing protease inhibitor cocktail (Roche diagnostics, # 11-836-170-001), 1 mM PMSF ...
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bioRxiv - Immunology 2024Quote: ... and 0.5% Triton X-100) with 1x protease and phosphatase inhibitors (c0mplete™ Protease Inhibitor Cocktail, Roche, product number 04693159001; PhosSTOP™, Roche, product number 04906837001). The resulting lysate was transferred to a labeled 1.5mL tube and placed on ice ...
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bioRxiv - Cell Biology 2020Quote: The rat pancreas was isolated following injection of 1 mg/mL collagenase P (Roche, Indianapolis, IN, USA) through the common bile duct ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM ß-mercaptoethanol (BME)) supplemented with 1 mM phenylmethylsulfonyl fluoride and 10 μg/mL DNase (Roche). The resuspension was processed with an Emulsiflex-C5 homogenizer (Avestin) ...
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bioRxiv - Cell Biology 2020Quote: ... PDMS stamps were prepared from wafer molds and coated with mixture of 50 μg/mL fibronectin (Roche) and 25 μg/mL Atto647-conjugated fibronectin (Atto dye ...
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bioRxiv - Immunology 2021Quote: ... Lamina propria lymphocytes were isolated by digesting the remaining tissue in 1 mg/mL collagenase D (Roche) and 20 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Square plates containing standard Middelbrook 7H10 OADC agar were prepared and supplemented with kanamycin (Roche,20ug/ml), with or without anhydrotetracycline (ATc ...
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bioRxiv - Biophysics 2022Quote: ... approved by the Danish Veterinary and Food Administration) and digested with collagenase (1.5 mg ml−1, Roche), dissolved in OR2 (82 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was treated with 1 ml of Red Blood Cell Lysis Buffer (Roche, Solna, Sweden) for five minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Single oocytes in PCR tubes were thawed and incubated with 0.2 mg/mL of DNase I (Roche) at 37°C for 30 min on a shaker ...
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bioRxiv - Synthetic Biology 2019Quote: ... previously supplemented with one tablet (per 10 ml of buffer volume) of complete protease inhibitor cocktail (Roche). Cell suspensions were incubated on ice for 15 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Lymphocytes were isolated from tumor tissue by mechanical homogenization and digestion with collagenase A (1mg/ml, Roche) and DNAase I (0.5ug/mL ...
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bioRxiv - Microbiology 2019Quote: ... pH 8.0) containing 1 mg/mL lysozyme and one complete mini EDTA-free protease inhibitor pellet (Roche). Cell suspensions were incubated on ice for 20 min before sonication ...
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bioRxiv - Immunology 2019Quote: ... liver tissue was minced and digested in an enzyme cocktail 0.625 mg/ml collagenase D (Roche, 11088882001), 0.85 mg/ml collagenase V (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2019Quote: ... The Th17 cultured cells were stimulated in presence of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Biochemistry 2019Quote: ... and then scraped in 10 ml PBS + 1X Protein Inhibitor Cocktail buffer (catalog no. COUL-RO, Roche) and centrifuged for 5 min at 1,000 x g at 4°C ...
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bioRxiv - Immunology 2021Quote: Mouse lung cells were obtained by digestion of lung tissue with 0.05 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and then dissociated with gentleMACS dissociator (Mylteni) and dissociated in RPMI containing 0.167mg/mL liberase TL (Roche), 0.25mg/mL DNase I (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Tissue fragments were homogenized with gentleMACS (Miltenyi) and digested in RPMI with Liberase TL 0.1mg/mL (Roche) and DNase I 0.1mg/mL (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Quad tissue samples were finely diced and digested with 1.67 Wünsch U/mL Liberase TL (Roche Diagnostics) and deoxyribonuclease I (0.2 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was filtered through a 70 µm filter with DNAse (66 U/mL) (Roche Diagnostics). Cell pellet was resuspended in 25% density gradient and centrifuged at 521 g for 20 min at 18° C ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4 supplemented with 1 µg/mL Dnase I and cOmplete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 mM DTT) containing 200 µg/mL lysozyme and an EDTA-free protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 mM imidazole) containing 200 µg/mL lysozyme and an EDTA-free protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... and enzymatic digestion was performed in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 0.2mg/mL collagenase D (Roche), 0.01mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... E15.5 fetal stage thymi were dissected and digested in 1 mg/ml collagenase/dispase (Roche, Basel, Switzerland) and passed through a 100 μm mesh to remove debris ...
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bioRxiv - Biochemistry 2022Quote: ... Tubes were placed on a magnet stand to remove unbound antibody and washed three times with 1 ml digitonin buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... a mix of enzymes was used (collagenase V, Sigma, C9263-1G, 0.85 mg/ml; Collagenase D, Roche, 11088882001 ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed twice with 1 ml PA-150 buffer containing EDTA-free protease inhibitor cocktail (Roche) (500 × g ...
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bioRxiv - Immunology 2020Quote: ... harvested back skin was minced with scissors in RPMI medium containing 0.1 mg/ml DNase I (Roche) and 1mg/ml Collagenase I (GIBCO) ...
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bioRxiv - Immunology 2020Quote: Skin samples from mice were enzymatically digested to release cells using 1 mg/ml collagenase D (Roche), 0.5 mg/ml dispase (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear staining was performed by adding 1 μg/mL DAPI in the buffer solution (10236276001, Roche Diagnostics). After incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Genetics 2021Quote: ... supplemented with Complete Mini EDTA-free Protease inhibitor tablets (46548400, Roche, ½ tablet per 5 mL lysis buffer) and phosphatase inhibitors (CA80501-130 ...
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bioRxiv - Immunology 2022Quote: ... Livers from day P14 mice were enzymatically digested using 2.5 mg/mL liberase (Roche Indianapolis, IN, 05401119001) in 1 M CaCl2 HEPES buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... PBS was aspirated and 5 ml 0.25% pre-warmed trypsin-EDTA with 10 U/µl DNaseI (Roche) was added and put into a 37°C water bath for 30 minutes with gentle inversion every 5 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Bound biotin peptides were then detected by incubating with 1 U/ml of HRP-conjugated streptavidin (Roche) on ice for 45 min in PBS/0.1% Triton X-100 ...