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Citations for Roche :
351 - 400 of 553 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in ice-cold lysis buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, supplemented with a protease cocktail inhibitor from Roche, Switzerland) for 30 minutes on ice ...
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bioRxiv - Developmental Biology 2020Quote: Transfected HEK cells were lysed in cold immunoprecipitation buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) with protease inhibitors (Roche), and centrifuged for 10 minutes and 3,000 rpm at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and the cell pellet resuspended in cold lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 1% Triton X-100, cOmplete protease inhibitor cocktail, Roche) and incubated on ice for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate (modified RIPA) plus a Complete Protease Inhibitor Cocktail (Roche Applied Science) on ice ...
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bioRxiv - Cell Biology 2021Quote: ... PH7.4, 150 mM NaCl, 1 mM MgCl2, 20% glycerol, 0.2% NP-40, and 1X protease inhibitor cocktail from Roche) for protein extraction ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ml NP-40 in a total volume of 500 ml ddH2O) containing phosphatase and protease inhibitor cocktail (Roche) then centrifuged for 30 min at 12000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1% NP-40, and a protease inhibitor cocktail tablet [Roche]). After clarifying lysates by centrifugation at 2,000 rpm for 1 min at 4 °C twice ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
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bioRxiv - Neuroscience 2022Quote: ... previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5, 150 nM NaCl, 1% NP-40, 1x protease inhibitors (Roche)) for 3 times ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell extracts were prepared by lysing cells in buffer X (50 mM Tris pH 8.5, 250 mM NaCl, 1 mM EDTA, 1% NP-40, protease inhibitor minitablet (Roche)) and quantified using Bradford assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was dissolved in lysis buffer 2 (1x HEMG with 200 mM NaCl, 0.5% NP-40, 1x protease inhibitor cocktail (Roche), 0.5mM PMSF ...
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bioRxiv - Genetics 2022Quote: ... resuspended in lysis buffer (25 mM Tris-HCl at pH 7.4, 150 mM NaCl, 10% glycerol, 1% NP-40, Roche Complete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.5% NP-40, 1 mM DTT, 1X protease inhibitor [EDTA-free complete Protease Inhibitor Cocktail, Roche, 11873580001] and 1:100 phosphatase inhibitor [Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were lysed in whole-cell extraction buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1% NP-40) supplemented with proteinase and phosphatase inhibitors (Roche). The lysates were cleared by centrifugation at 14,000 rpm for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in cold lysis buffer (10 mM Tris pH 8, 10 mM NaCl, 0.2% NP-40, 1x protease inhibitors (Roche)) and incubated for 20 min on ice ...
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bioRxiv - Plant Biology 2022Quote: ... and extracted in 4 ml of freshly prepared Extraction Buffer (50 mM HEPES pH 8, 150 mM NaCl, 0.3% NP-40, 1x cOmplete ULTRA protease inhibitor - EDTA (Roche), 1mM PMSF ...
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bioRxiv - Plant Biology 2022Quote: ... PHB variant complexes were captured using 25 μl Protein A Dynabeads and washed 4x with freshly prepared Wash Buffer (50mM HEPES pH 8, 450mM NaCl, 0.1% NP-40, 1x cOmplete ULTRA protease inhibitor - EDTA (Roche), 1mM PMSF ...
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bioRxiv - Neuroscience 2022Quote: Bacterial pellets were re-suspended in 15 mL lysis buffer (50 mM Trizma base, 120 mM NaCl, 0.5% NP-40) containing cOmplete Mini protease inhibitor cocktail (Roche) and DNAseI (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected 24 hrs after transfection and lysed using a lysis buffer (25mM Tris/Cl pH 7.5; 150mM NaCl; 0.5M EDTA; 0.5% NP-40, 2.5% Glycerol, Roche complete protease inhibitors). The lysates were then incubated with 15 µl anti-FLAG M2 magnetic beads (M8823 ...
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bioRxiv - Cell Biology 2022Quote: ... which were pre-washed with NP-40 lysis buffer with protease/phosphatase inhibitors (Roche, cat# 11697498001 and cat# 4906845001) and incubated for 2.5 hours at +4C° with gentle agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM NaCl and 0.2% NP-40) supplemented with protease inhibitor cocktail (cOmplete ULTRA tablets, Mini, EDTA -free, EASYpack, Roche) and kept shaking on rotor for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 400U Ribolock inhibitor, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using NP-40 lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... The soluble nuclear fraction was collected by incubation with the nuclear buffer (10mM HEPES pH7, 200mM NaCl, 1mM EDTA, 0,5% NP-40) supplemented with protease inhibitors cocktail (Roche) for 10min on ice and centrifuge at 14000rpm for 2min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM EDTA, 0.1 % NP-40, 20 mM KCl, 150 mM NaCl, plus a protease inhibitor cocktail from Roche) and centrifuged for 2 minutes and 2,500 g at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 140 mM NaC1, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail [Complete; Roche]). The cell pellet was flash frozen in liquid nitrogen with 1.5 mL of lysis buffer and grounded in a Spex Freezer Mill 6775 to a fine powder ...
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% (v/v) NP-40 and 0.5 mM Tris carboxy ethyl phosphene (TCEP) buffer supplemented with protease inhibitor cocktail (Roche), flash-frozen in liquid nitrogen and lysed under cryogenic conditions using a Retsch MM400 (Retsch) ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Microbiology 2024Quote: ... gondii RH tachyzoites lysed in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris [pH 8.0]) supplemented with complete protease inhibitor (Roche). The insoluble material was removed from the lysate by centrifugation at 10,000 x g for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once with PBS and then lysed with 0.5% NP-40 in PBS supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail (Roche) and Benzonase (Merck Millipore ...
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bioRxiv - Genetics 2023Quote: ... 10% Glycerol, 0.2% NP-40, 5mM MgCl2, 5mM DTT, containing one tablet per 10 ml of protease inhibitor cocktail, Roche). Cell debris was removed by centrifugation at 12 Krpm at 4°C for 20 min and the supernatant was recovered ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Developmental Biology 2020Quote: ... 50mM Tris-HCL pH 7.4 with 150mM NaCl plus 1.0% NP-40 and EDTA-free protease inhibitor (Roche cOmplete, #11697498001). SDS page was run on precast 8–12% bis-tris gel (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Immunology 2021Quote: Stimulated cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche #11836170001), 1 mM Na3VO4 ...