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Citations for Roche :
301 - 350 of 553 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 300 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 2 mM EDTA, containing ‘Complete mix’ protease inhibitors from Roche) and passed 10 times through a 26-gauge needle ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell extracts were prepared by lysing cells in RIPA buffer (50 mM Tris HCl pH 7.4/150 mM NaCl/0.5% sodium deoxycholate/0.1% sodium dodecyl sulfate/1% NP-40) containing cOmplete protease inhibitor cocktail (Roche), followed by quantification using Bradford assays ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using the LightCycler Multiplex RNA Virus Master Kit (Roche Diagnostics, Mannheim, Germany) on a Cobas z480 analyzer (Roche Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... Cellular mRNA of cells not exposed to virus was isolated with an mRNA Capture kit (Roche) and cDNA was synthesized with a reverse-transcriptase kit (Promega) ...
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in 1x lysis buffer (1% NP-40, 100mM Tris, pH 8.0, and 150mM NaCl) containing a protease inhibitor mixture (Roche) and shaken 60 mins at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 100 mM NaCl; 1 mM EDTA, 1% Triton-X or 0.1% NP-40; supplemented with Complete Mini protease inhibitors, Roche). Cell lysates were cleared by centrifugation (3x 10 min at 20000 g at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 10 µl reaction mix was mixed with 290µl IP buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) containing protease inhibitor cocktail tablet (Roche) and 2 µg/ml of anti-FLAG (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: 5 μl of antiserum were diluted with 250 μL of PBS containing 0.05% NP-40 and coupled to 10 μl of Protein A-Agarose beads (Roche) by rotating at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v))% NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% NP-40 and 1 mM EDTA supplemented with 1 × protease inhibitor complete mini EDTA-free cocktail from Roche). The supernatants of cell lysates were boiled for 5 min in 1 × SDS loading buffer (6 × ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with cold PBS and lysed for 20 minutes at 4°C in lysis buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40, supplemented with cOmplete proteinase inhibitors (Roche)) prior to snap freezing in 1ml lysis buffer on dry ice ...
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bioRxiv - Cancer Biology 2019Quote: ... the cell pellets were resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 % NP-40, 5 mM MgCl2, 5 % glycerol and protease inhibitor cocktail [Roche]) and incubated for 20 min at 4 °C with end-over-end rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were rinsed twice with PBS and resuspended in 1 mL lysis buffer (1X PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, #11836170001). Chromatin was sheared to 200-500 base pairs using a Covaris E220 sonicator and cleared by centrifugation for 15 minutes at 19,000 × g ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in NP40 buffer (50mM Tris-HCl [pH 8.0], 280mM NaCl, 0.5% NP-40) supplemented with cOmplete™ protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Genomics 2019Quote: ... To obtain nuclei the resulting parasite pellet was lysed with cell lysis buffer (20 mM Hepes, 10mM KCl,1mM EDTA, 1mM EGTA, 0.65% NP-40, 1mM DTT, 1x protease inhibitor (Roche)) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol, Roche Complete Protease Inhibitors and PhosSTOP Protein Phosphatase Inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... 1% v/v NP-40, 0.1% w/v SDS, 1% w/v sodium deoxycholate, 1× complete mini-protease mixture; Roche), and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM KCl, 5 mM MgCl2, 0.01% NP-40, freshly prepared 20 mM Na2MoO4, and cOmplete protease inhibitors; Roche). 10-20 million cells were lysed in 0.5 mL Felts buffer by rotation at 4°C for 30 minutes and vortexing for 10 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... in lysis buffer (150 mM NaCl, 1% NP-40, 50mM Tris pH 8.0, 1x Roche protease inhibitor tablet (11697498001)) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted with an equal volume of IP buffer (150 mM HEPES-KOH pH 6.8, 150 mM K2OAc, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 1% NP-40, 1x Roche protease inhibitor cocktail and 1 x phosSTOP cocktail ...
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bioRxiv - Microbiology 2020Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v) % NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
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bioRxiv - Microbiology 2021Quote: ... 60 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 300 mM sucrose, 0.4% NP-40, 1 mM DTT, 1x Roche Complete protease inhibitors cocktail EDTA-free ...
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bioRxiv - Plant Biology 2021Quote: ... 150 mM NaCl, 10% glycerol, 0.2% NP-40, 1 mM EDTA, 5 mM DTT, and EDTA-free protease inhibitor [Roche]). Anti-GFP agarose resin (Chromotek ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell lysates from cell lines were prepared using NP40 lysis buffer [20 mM Tris-Cl pH 7.8‚137 mM NaCl and 1% NP-40 supplemented with 1X protease inhibitor cocktail (Roche)].The tissue lysate was prepared from mouse ...
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bioRxiv - Cancer Biology 2020Quote: The tumor tissue was weighed and ice-cold NP-40 buffer including a protease inhibitor cocktail (cOmplete inhibitor, Roche) was added in order to prepare a 2.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in immunoprecipitation buffer (50mM Tris–HCl, 0.5% NP-40 PBS with protease inhibitor cocktail (Roche)) 24hr after transfection and subjected to GFP-trap (ChromoTek ...
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bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were washed five times with the IP buffer (10mM Tris pH8.0, 150mM NaCl, 0.01% NP-40, 1mM MgCl2, 1X Protease inhibitor (Roche,11836170001), 5% Glycerol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 mM protease inhibitor cocktail (1 mM EDTA, 20 mM NaF, 0.5% NP-40, 1% Triton X-100) (Roche) and 0.5% Na3VO4 on ice ...
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bioRxiv - Neuroscience 2022Quote: ... buffer (50mM Tris-HCl pH = 7.4, 1% NP-40 / Igepal, 100mM NaCl, 2mM MgCl2) supplemented with Proteinase Inhibitor (Roche) und Phosphatase Inhibitor (ThermoScientific) ...
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bioRxiv - Cell Biology 2022Quote: Cell pellets were lysed for 30 min at 4°C with end-over-end mixing in cold lysis buffer [50 mM tris pH 7.5 | 150 mM NaCl | 1 mM EDTA | 0.5% NP-40 | 1x protease (Roche, 11836170001) and phosphatase (Roche ...
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bioRxiv - Microbiology 2020Quote: 0.5 to 1 million cells were lysed in 100 μL of RIPA buffer (50mM Tris HCl, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1% NP-40, Protease inhibitor (Roche; 1187358001)) ...
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bioRxiv - Neuroscience 2020Quote: ... Powdered brains were then resuspended in 1mL ice cold 1X Lysis Buffer(50mM Tris-HCl pH 7.4, 100mM NaCl, 1%NP-40, 1X Roche c0mplete EDTA-free protease inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with lysis buffer (final concentration: 50 μg ml−1 lysozyme, 0.8% NP-40, 1 × protease inhibitor (Roche), 250 U ml−1 benzonase ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were incubated in 1 ml ice-cold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Roche) for 15 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% NP-40, 0.5% TX-100, 150 mM NaCl plus complete EDTA-free Protease Inhibitor Cocktail Tablets, Roche, #11697498001), diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell extracts were prepared by rotating the cell pellets for 2 hrs at + 4°C in five volumes of the high salt lysis buffer (50 mM Tris, 300 mM NaCl, 10% glycerol, 0.5% NP-40, 1x cOmplete ULTRA protease inhibitors (Roche)) ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in GFP trap buffer (50 mM Tris-HCl, 0.5% NP-40 and Roche protease inhibitor cocktail) and the lysate was added to pre-equilibrated GFP trap beads (ChromoTek) ...
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bioRxiv - Microbiology 2021Quote: ... and LiCl (250 mM LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 20 mM Tris-HCl PH8.0, complete protease inhibitor [Roche]) washing buffers ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.2ml of Buffer NUN2 (300mM NaCl, 7.5mM MgCl2, 1% NP-40, 1M Urea supplemented with complete EDTA free protease inhibitor (Roche) and PhoSTOP (Roche ...