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Citations for Roche :
251 - 300 of 553 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Cells were harvested and lysed in NP-40 buffer (50 mm TRIS, pH 8.0, 150 mm NaCl, 1.0% NP-40 with Complete Protease Inhibitor Cocktail from Roche). For Western blots ...
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bioRxiv - Genetics 2020Quote: ... and suspended in 1 mL of ice-cold protein extraction buffer (50mM HEPES pH 7.5, 150mM NaCl, 0.02% NP-40, 1mM EDTA, 1mM phenylmethylsulfonyl fluoride [PMSF; Sigma, P7626], one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail [Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Microbiology 2020Quote: Intracellular expression of APOBEC3 proteins during virion production was determined by lysis of the virion-producing 293T cells with NP-40 buffer with protease inhibitor (200mM NaCl, 50mM Tris pH 7.4, 0.5% NP-40 Alternative, 1mM DTT, and Roche Complete Mini ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were generated by lysing cells in RIPA buffer (50 mM Tris-Cl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1× Roche complete mini-protease inhibitor cocktail ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% NP-40 in 1X PBS) plus protease inhibitor (cOmplete, Mini Protease Inhibitor Cocktail Tablets, EDTA free, Roche) on ice for 10 minutes and clarified by centrifugation at 15,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... and cells were lysed in plate in RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1x Roche protease inhibitor tablet [no EDTA] ...
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bioRxiv - Genomics 2021Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Genomics 2021Quote: ... Pellets were resuspended in 500 μL cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, 1X protease inhibitor (Roche)) and rotated for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei isolated from approximately 2.5x107 cells were resuspended in one ml of extraction buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT, 0.25%NP-40, 1x Roche Complete protease inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... and pellets underwent a second lysis in high salt buffer (25mM HEPES-KCl pH7.9, 350mM NaCl, 10% w/v sucrose, 0.01% NP-40, 1X protease inhibitor cocktail from Roche, 20mM N-ethylmaleimide ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% NP-40) together with a cocktail of protease inhibitor (Complete EDTA-free, Roche Applied Science, Indianapolis, IN) followed by centrifugation at 14,000 rpm for 15 minutes at +4 °C to pellet the debris.
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bioRxiv - Biochemistry 2022Quote: ... resuspended in lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.05% NP-40, supplemented Complete EDTA-free [Roche]). After a brief sonication ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked pellets were thawed and gently resuspend in 1 mL cold Farnham Lab (FL) buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, 1X Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant was removed and nuclear pellet was washed once with 1 ml 1X FL buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, 1X Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated with immature and mature DC lysates prepared in RIPA buffer (10 mM HEPES, 50 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, protease inhibitor cocktail (Roche), 1 mM Na3VO4,1 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells where lysed in immunoprecipitation buffer (50 mM Tris-HCl, 0.5% NP-40, and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek) ...
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bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5, 150 mM NaCl, 0.5% [v/v] NP-40, and complete EDTA-free protease inhibitor cocktail [Roche]). The extracts were centrifuged at 20,000 × g at 4°C for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5, 150 mM NaCl, 0.5% [v/v] NP-40, and complete EDTA-free protease inhibitor cocktail [Roche]).
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl, 0.5% [vol/vol] NP-40, 1.0 mM EDTA, cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail – Roche) and rotation at 4°C for 30 min ...
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bioRxiv - Microbiology 2022Quote: Cells were washed once with 1xPBS before harvesting in NP-40 buffer with protease inhibitor (200 mM NaCl, 50 mM Tris pH 7.4, 0.5% NP-40 Alternative, 1 mM dithiothreitol, and Roche Complete Mini ...
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bioRxiv - Microbiology 2022Quote: ... Cells were combined and lysed in 1 ml co-IP buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 0.5% NP-40, 1X cOmplete protease inhibitor (Roche)) and divided into equivalent fractions for ICC ...
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bioRxiv - Microbiology 2022Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: Total proteins were extracted from 6 g of frozen ground Arabidopsis seedling tissue with IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% Triton, EDTA-free protease inhibitor cocktail [Roche]). Lysates were cleared by centrifugation (6,000 g ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with maltose (20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM EDTA, 0.1 % NP-40, 150 mM NaCl, 0.5 mM DTT, plus a protease inhibitor cocktail from Roche; used in pull-down experiments with the GFP-Trap resin of Proteintech) ...
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bioRxiv - Cell Biology 2023Quote: Cells were re-suspended in 0.1% NP-40-PBS (1ml/1×107 cells) with 1X Protease Inhibitors (Roche) and 1mM DTT ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 2x Complete EDTA-free protease inhibitor [Roche]), and insoluble material and lipid were cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed three times with 200 µL of washing buffer (50 mM Tris-HCl at pH 7.5, 150 mM KCl, 5 mM MgCl2, 0.05% NP-40, EDTA-free cOmplete mini protease inhibitor [Roche, 11836153001] ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) using 300 μg/mL lysozyme for 20 minutes followed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... The final mitochondria enriched pellet was resuspended in 250μl of Hepes lysis buffer (50mM Hepes pH 7.4, 0.5% NP-40) supplemented with complete protease inhibitor cocktail (Roche). Total protein was quantified with the Bradford method ...
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bioRxiv - Biochemistry 2023Quote: ... 10% glycerol and 0.1% NP-40 supplemented with 0.1 mg of lysozyme/ml and complete inhibitor cocktail (Roche)) and sonicated to disrupt the cells ...
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bioRxiv - Neuroscience 2023Quote: ... the striatal/accumbal slices were sonicated in 300 µL lysis buffer (50 mM Tris-HCl [pH 7.5], 1 mM EGTA, 20 mM MgCl2, 500 mM NaCl, 0.5% NP-40, protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2023Quote: ... and lysed in NP-40 buffer (150 mM NaCL, 1% IGEPAL, 50 mM Tris-Cl p.H. 7.5) with protease inhibitor (Roche #04693132001 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 µM Olaparib-containing medium for the 48 h before being washed with PBS and pre-extracted with pre-extraction buffer (10 mM Tris-HCl, 2.5 mM MgCl2, 0.5% NP-40, 100× Protease inhibitor cocktail (Roche)) for 5 min at 4°C ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... 0.1% NP-40) containing 2 μM MG-132 and cOmpleteTM mini EDTA-free protease inhibitor cocktail (Roche, #04693159001) for 30 minutes at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were extracted from 2-3 aliquots of 3 g frozen ground Arabidopsis seedling tissue with IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% Triton-X, EDTA-free protease inhibitor cocktail [Roche]). Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 300 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.075% NP-40, 1x protease/phosphatase inhibitor cocktails, Roche) and incubated for 10 minutes at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell extracts were prepared by lysing cells in RIPA buffer (50 mM Tris HCl pH 7.4/150 mM NaCl/0.5% sodium deoxycholate/0.1% sodium dodecyl sulfate/1% NP-40) containing cOmplete protease inhibitor cocktail (Roche), followed by quantification using Bradford assays ...