Labshake search
Citations for Roche :
201 - 250 of 553 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed by bead beating after resuspension in IP-Lysis Buffer (10 mM Tris.Cl pH 8.0, 100 mM NaCl, 10% glycerol, 0.1% NP-40, and Roche cOmplete™ EDTA-free protease inhibitor cocktail) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.1% NP-40 and 5 mM beta-mercaptoethanol) supplemented with Complete EDTA-free Protease Inhibitor Cocktail (Roche). The suspension was flash frozen in droplets ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5% NP-40) supplemented with PhosStop and Complete Ultra Protease Inhibitor tablets (Roche, Welwyn Garden City, UK). Lysates were incubated on ice for 10 minutes and centrifuged ...
-
bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl and 0.5% NP-40 with 1x cOmplete protease inhibitor cocktail and PhosSTOP tablets (Roche); for UB-Trap ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 mM MgCl2; 0.1 % NP-40; 0.2 U/µl RNaseOUT; 0.32 M Sucrose; 1x protease inhibitor, Roche) and transferred to 2 ml Dounce tissue grinder (Sigma) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were homogenised in ice cold lysis buffer (10 mM Tris/HCl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5 % NP-40, and EDTA-free protease inhibitor cocktails; Roche) for 15 min on ice and the cell lysate was centrifuged at 13000 RPM for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
-
bioRxiv - Neuroscience 2023Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
-
bioRxiv - Neuroscience 2023Quote: ... and 100,000 worms were collected in 1 ml of RIPA buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
-
bioRxiv - Neuroscience 2023Quote: ... in lysis buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, complete protease inhibitors, Roche) and 4 U per ml Turbo-DNase (Thermofisher) ...
-
bioRxiv - Genetics 2023Quote: ... Ovaries or testis were added to 1 mL of cold lysis buffer (50 mM Tris-HCl pH 8.0, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 0.1 mg/mL PMSF, Roche complete EDTA-free protease inhibitor tablet ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1.0% w/v NP-40) supplemented with cOmplete ULTRA EDTA-free protease inhibitor cocktail (#05892953001, Roche). Samples were homogenized and centrifuged at 20,000 ×g ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5 mM MgCl2, 10 mM KCl, 0.25% NP-40, 0.5 mM DTT, 2x protease inhibitor cocktail/Roche), incubated for 10 min on ice ...
-
bioRxiv - Microbiology 2020Quote: Quantification of the virus by qPCR was performed using the LightCycler 480 System (Roche, France) and specific primers designed in the non-structural gene NS1 (qAalDV2-F ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then lysed with IP lysis buffer (0.5% NP-40, 150 mM NaCl, 50 mM Tris HCl, pH 7.5, protease inhibitors [cOmplete, Roche] ...
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
-
bioRxiv - Cell Biology 2019Quote: ... pH 6.9) containing 0.5% (v/v) NP-40 and protease inhibitors (cOmplete ULTRA Tablets, Mini, EDTA-free, Roche). The tube is left for 15 min at room temperature with gentle mixing ...
-
bioRxiv - Immunology 2021Quote: ... cells were lysed in ice cold RIPA buffer (50mM Tris HCL, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1%NP-40, EDTA-free protease inhibitor (Roche), phosphatase inhibitors (Halt phosphatase ...
-
bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
-
bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
-
bioRxiv - Molecular Biology 2021Quote: ... Adipocytes were washed and lysed in a buffer (10mM Tris-HCl pH8.0, 10mM NaCl, 0.2% NP-40, 1x cOmplete Protease Inhibitor Cocktail from Roche) on ice for 30 min with shaking ...
-
bioRxiv - Microbiology 2019Quote: ... Crosslinked cell pellets were mixed with 1 mL fractionation buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40 with cOmplete EDTA-free Protease Inhibitors [Roche]) and incubated on ice for 10 min ...
-
bioRxiv - Microbiology 2019Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 × g for 10 min at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40 and 0.5% SDS supplemented with protease inhibitor [Roche]) and centrifuged at 12,000 g at 4°C for 10 min ...
-
bioRxiv - Genetics 2019Quote: Cells were lysed in EBC buffer (50 mMTris pH 7.5, 120 mMNaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II ...
-
bioRxiv - Cancer Biology 2021Quote: Transfected HeLa were harvested and lysed with lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, protease inhibitors tablet from Roche, 50 mM NaF ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 5mM MgCl2, 0.5% NP-40, pH 7.4, supplemented with Complete Protease Inhibitor Cocktail, Roche). After centrifugation at 5000g for 10minutes ...
-
bioRxiv - Microbiology 2021Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
-
bioRxiv - Cancer Biology 2020Quote: The PKO cells were incubated with HEPES buffer (40 mM HEPES pH 7.5, 150 mM NaCl, 0.1% NP-40) supplemented with protease (Roche) and phosphatase (Beyotime ...
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: Cells transfected with indicated constructs were harvested and lysed in NETN buffer (10mM Tris-HCl [pH 8.0], 100mM NaCl, 1mM EDTA, and 0.5% NP-40) plus protease inhibitor (Roche) on ice for 30 min ...
-
bioRxiv - Genetics 2022Quote: ... 0.05% NP-40) + protease inhibitors (Complete Ultra Tablets, Mini Protease Inhibitor Cocktail; Roche #05892791001 and 1mM PMSF; Sigma). Worms were allowed to settle at the bottom of the tube and the lysis buffer was removed until there was 0.5-1ml buffer covering the worm pellet ...
-
bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris, 0.1% v/v SDS, 0.5% v/v NP-40 pH8, 1x complete protease inhibitor, Roche) with stainless metal beads (Qiagen ...
-
bioRxiv - Immunology 2021Quote: ... the cells were thawed on ice followed by lysis using Farham lysis buffer (5 mM PIPES pH 8.0, 85mM KCl, 0.5% NP-40 supplemented with protease and phosphatase inhibitors (Roche)) made in miliQ ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was aspirated and the pellet was resuspended in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with Roche protease and phosphatase inhibitor tablet just before use) ...
-
bioRxiv - Genomics 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
-
bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS)39 ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was removed and the pellet was dissolved in 500 µl of ice-cold nuclear solubilization buffer (25 mM HEPES [pH 6.8], 1 M sorbitol, 0.5% NP-40, 250 μg/ml PMSF, cOmplete [EDTA-free protease inhibitor cocktail; Roche]). Following incubation for 30 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: Crosslinked nuclei were extracted in 0.8ml modified RIPA lysis buffer (50mM Tris, 150mM NaCl, 0.5% sodium deoxycholate, 0.2% SDS, 1% NP-40, supplemented with: 1x antiprotease (Roche), RNasin (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed with Farnham Lysis Buffer (5mM PIPES at pH 8.0, 85 mM KCl, 0.5% NP-40) containing protease inhibitor (Roche). Cell lysate was centrifuged at 2,000 rpm for 5 min at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: Cells were lysed in 1× lysis buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) by rotating for 30 min at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA and 0.5% NP-40 supplemented with Complete Mini EDTA-free Protease inhibitor cocktail tablets (Roche)) and left to incubate for 20 minutes on ice ...
-
bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
-
bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, Roche mini-tablet protease inhibitor ...