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Citations for Roche :
3601 - 3650 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated with DAPI solution (0.5 mg/mL, Roche LifeScience, Penzberg, Germany) as a DNA fluorescence probe for 10 min ...
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bioRxiv - Biophysics 2024Quote: ... 10 μg/mL DNase I and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche) and lysed using Avestin® Emulsiflex-C3 homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... c0mplete™ EDTA-free Protease Inhibitor Cocktail (04693l3200l, Roche, l tablet/l0 ml) and PhosST0P (490684S00l ...
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bioRxiv - Biochemistry 2024Quote: ... 30 ml of additional lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Physiology 2024Quote: ... minced and incubated with a mix of 2.4U/ml Dispase II (Roche, #04942078001) and 100μg/ml collagenase A (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... Selection of cells expressing the variants was performed using Geneticin 2mg/ml (Roche). Stable overexpression of the variants was confirmed via qPCR using human HMGCR Taqman probes.
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with 75 mU/mL Anti-Digoxigenin-AP (#11093274001, Roche) in blocking solution for 45 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cph1-PM-MDA cells were maintained in 1800 μg/mL G418 (Geneticin, Roche) and used for up to 10 passages.
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bioRxiv - Cell Biology 2024Quote: ... The minced hearts were transferred to digestion buffer (20mM BDM, 1.5mg/ml Roche Collagenase/Dispase enzyme mix in Leibovitz L15 medium)(Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... minced and digested in HBSS containing 0.5 mg/ml Collagenase P (Roche, 11249002001) for 15 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... one ear was injected intradermally with 25µL of 100µg/mL alum hydroxide (Roche), and the contralateral ear was left untreated ...
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bioRxiv - Immunology 2024Quote: ... Skin samples were minced and digested with 0.28 units/ml Liberase TM (Roche) and 0.1 mg/ml of Deoxyribonuclease I (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by discarding 100 ul from each well and adding 100 ul of solution containing DMEM with 10% FBS and WST-1 reagent (Roche Diagnostics) diluted 1:10 ...
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bioRxiv - Immunology 2021Quote: ... 20 mM Tris pH 7.5, 1% Triton X-100 supplemented with 1× protease inhibitor and 1× phosphatase inhibitor; Roche, Basel, Switzerland). Lysates were cleared by centrifugation at 10,000 r.p.m ...
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bioRxiv - Cell Biology 2020Quote: ... targeting the indicated genes (Table S2) at a final concentration of 50 nM or 100 nM using X-tremeGene siRNA transfection reagent (Roche, 04476093001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in lysis buffer (100 mM Tris pH 8, 300 mM NaCl, 5ml Mg2Cl, 1mM ß-mercaptoethanol and protease inhibitor tablets (complete tablets, Roche) and lysed by sonication ...
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bioRxiv - Physiology 2022Quote: ... 2 mmol/l MgCl2, 10 mmol/l NaF, 1 mmol/l PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 30 min on ice and centrifuged at 20,000 g for 10 min at 4ºC ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton-X-100) supplemented with 1x protease inhibitor (Complete mini EDTA-free, Roche, Switzerland and 1x phosphatase inhibitor (PhosStop, Roche, Switzerland) on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... A two-hundredth aliquot of the cDNA was subjected to qRT-PCR with primers (final concentration 200 nM each) and MGB probe (final concentration 100 nM; the Universal Probe Library [UPL], Roche Diagnostics) sets as shown in Supplementary File 4 for human ACE2 ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in HM, resuspended in lysis buffer (SDS 2%, Tris-HCl pH 8.0 100 mM containing a mix of protease (Roche complete cocktail) and phosphatase inhibitors (Biotools cocktail or lab-made cocktail ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 cycles of primary PCR to amplify the region of interest was performed using 2 μL of DNeasy eluate (∼100–300 ng template) in a 5 μL Kapa HiFi HotStart polymerase reaction (Kapa Biosystems; for primers see Supplementary Data Set 1) ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed five times and incubated with 100 µl/well goat anti-human IgG conjugated with alkaline phosphatase (Roche Diagnostics) diluted 1 in 5,000 for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... and samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). Customized adapters with unique molecular indexes (UMI ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were prepared using 100-500 ng of cell free DNA (cfDNA) or sonicated genomic DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Resulting cell pellets were resuspended in Buffer A (100 mM NaH2PO4, 10 mM Tris, 6 M GuHCl, 10 mM imidazole) + PIC (Roche 05056489001). Cells were sonicated 3X at 25% power for 10s (Cole-Parmer GE 130PB-1) ...
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bioRxiv - Biochemistry 2020Quote: ... and resuspended in 100 μL of 50 mM Tris-HCl (pH 7.6)/250 mM sucrose buffer supplemented with protease inhibitors (Roche Diagnostics GmbH). Cells were disrupted by 20 passages through a 27-gauge needle ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM MgCl2; 2 mM EGTA pH 8.0; 0.1% Triton X-100; 0.1 mM PMSF; 1x Roche Complete protease inhibitors cocktail) supplemented with increasing NaCl concentrations (80-600 mM ...
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bioRxiv - Physiology 2021Quote: Tissues were homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
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bioRxiv - Biophysics 2022Quote: ... resuspended in Buffer A (20 mM Tris pH 7.5, 100 mM NaCl) with complete EDTA-free protease inhibitor (Roche, Indianapolis, IN), and lysed using an Avestin C5 emulsifier at ∼15,000 psi ...
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bioRxiv - Genomics 2022Quote: The cell pellet was resuspended in 500 μL of cold permeabilization buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% IGEPAL CA-630, 100 μL of protease inhibitor cock-tail Roche Cat no. 11836170001) and placed on ice for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, 10 mM Sodium butyrate, 0.1% deoxycholate, 0.1% SDS, 1% Triton X-100, 1mM PMSF and 1 × Roche protease inhibitor Cocktail). The obtained chromatin was sonicated into DNA fragments between 200-500 bp using Diagenode Bioruptor (high setting ...
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bioRxiv - Plant Biology 2021Quote: ... 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with cold PBS and lysed using buffer A (PBS, 20 mM imidazole, 1% Triton X-100, and a cOmplete® protease inhibitor tablet (Roche). Lysates were clarified for 30 min at 13,000 g at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, 1x Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Biophysics 2019Quote: ... Pelleted cells were resuspended in Buffer A (20 mM Tris pH 7.5, 100 mM NaCl) with cOmplete EDTA-free protease inhibitor (Roche, Indianapolis, IN), and lysed using an Avestin C5 emulsifier at ∼15,000 psi ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed (10mM Tris-HCl [pH 8], 0.25% Triton X-100, 10mM EDTA, 100mM NaCl, Roche 1X Complete Protease Inhibitor) and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini, EDTA-free Protease Inhibitor Cocktail (Roche, #04693159001)) ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
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bioRxiv - Cancer Biology 2020Quote: ... 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1 mM PMSF, Roche protease inhibitors 11836170001) for 10 min at 4°C with rotation ...
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bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma], 10X protease inhibitors [Complete tablets, Roche, Indianapolis, IN]). Sucrose gradients for the preparation of lipid rafts were assembled previously described (18) ...
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bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-buffered saline (TBS) supplemented with 1% Triton X-100 and protease/phosphatase inhibitor cocktails (Roche Diagnostics), the insoluble material was cleared by centrifugation for 10 min at 20,000 × g ...
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bioRxiv - Microbiology 2021Quote: ... the beads were collected on a magnetic stand for 2 min and washed four times with lysis buffer and four times with wash buffer (50mM Tris pH 7.6, 300mM NaCl, 1% Triton X-100, + complete protease inhibitor/ Roche Cat# 11836153001). The beads were resuspended in 1x Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in 100 μl cold RIPA buffer (Pierce, 8990) supplemented with cOmplete mini EDTA-free protease inhibitor cocktail (Roche, 11836170001). Protein abundance was measured by Qubit ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei from 600,000 cells were isolated by hypotonic lysis in 1 mL buffer NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 0.5 mM spermidine; 0.1% Triton X-100; 20% Glycerol; Roche EDTA-free protease inhibitor) for 10 minutes on ice followed by centrifugation at 1300 xg for 4 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified using 22 cycles of PCR amplification from approximately 300 µg genomic DNA (10 µg DNA/100 µL reaction) using 2X KAPA HiFi HotStart ReadyMix (Roche KK2602), a combination of 8 staggered P5 primers ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were then isolated by incubating with NE1 buffer (10 mM HEPES pH7.9, 10 mM KCl, 0.1% Triton X-100, 20% glycerol, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 10 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad Laboratories GmbH ...