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Citations for Roche :
3601 - 3650 of 5262 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: mRNA was extracted at the indicated time points using the High Pure RNA Isolation kit (Roche, Mannheim, Germany). cDNA was generated using M-MuLV reverse transcriptase (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems. Libraries were sequenced in single-end using Illumina Hiseq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Microbiology 2019Quote: Transcriptional start sites of the virD and virB transcripts were obtained by using the 5’ RACE Kit (Roche), as described (Andrade et al ...
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bioRxiv - Immunology 2019Quote: ... RNAseq libraries were prepared as previously described 70 using KAPA Standed RNA-seq library preparation kit (KAPA Biosystems), and sequenced on an Hi-Seq 2500 platform (Illumina).
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bioRxiv - Immunology 2019Quote: ... TCRα and TCRβ PCR products were quantified using the KAPA library quantification kit for Illumina platforms (KAPA Biosystems) and pooled at the same concentrations ...
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bioRxiv - Microbiology 2019Quote: ... and H2O PCR grade (included in the Kit LightCycler® 480 Probes Master, Cat. No. 04707494001, Roche, Germany).
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bioRxiv - Genetics 2019Quote: ... The quantity of sequenceable library for each sample was determined using the KAPA Library Quantification Kit (Roche #KK4835) on the 7900HT real-time PCR (Applied Biosystems) ...
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bioRxiv - Genetics 2019Quote: ... with cDNA then being generated from 1 μg of RNA utilizing the First Strand Reverse Transcriptase kit (Roche). Quantitative PCR was utilized to assess target gene expression using the KAPA SYBR Fast QPCR Master Mix ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA-seq libraries were generated with 1ug of total RNA using KAPA mRNA HyperPrep Kit (KK8580, Kapa Biosystems) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... HIV-1 genomic RNA was extracted from supernatants containing virions using High Pure Viral RNA kit (Roche Inc.). SuperScript III and a primer downstream of the randomized regions were used to reverse transcribe viral RNA to cDNA ...
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bioRxiv - Cancer Biology 2020Quote: Libraries of ChIP DNA were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, Cape Town, South Africa) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Sequencing libraries were individually quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc.). Equimolar amounts of all libraries were pooled ...
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bioRxiv - Pathology 2020Quote: ... for 20 min at 37 °C and then visualized by the DAB Map detection kit (Roche Diagnostics KK). As the negative control the primary antibody was omitted and was being replaced by the non-immune serum ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Post-tagmentation gap filling and PCR amplification were performed using the KAPA HiFi HotStart PCR Kit (Kapa Biosystems) with the following cycling parameters ...
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bioRxiv - Clinical Trials 2019Quote: ... The pooled library was created with 50 ng DNA per sample and quantified using Kapa Kit (Kapa Biosystems). The pooled library was run on Illumina-MiSeq with 15% PhiX mock library to reduce discrepancies in read clustering ...
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bioRxiv - Neuroscience 2019Quote: ... Library quantification and quality checks were done using KAPA Library Quantification Kit for Illumina (KAPA Biosystems, Wilmington, MA), High Sensitivity DNA Kit on BioAnalyzer (Agilent ...
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bioRxiv - Genomics 2021Quote: ... Each entire ChIP sample was used to prepare Illumina sequencing libraries using the KAPA Hyper Prep Kit (Roche). Adapter-ligated DNA was separated on a 2% E-Gel EX (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... All libraries were quantified on the Agilent 2100 hsDNA BioAnalyzer and KAPA library quantification kit (Roche Cat# 07960140001). The resulting gene expression libraries were sequenced on an Illumina sequencing platform with a 28 × 8 × 60 bp configuration at a targeted depth of 25000 reads per cell ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 100 ng of total RNA was processed using reverse transcription kit to synthesize cDNA (Roche, Basel, Switzerland). The primer sequences used for RT-PCR analysis are listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems), pooled in equimolar quantities and single-end sequenced on a HiSeq 2500 platform ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR were performed using the LightCycler 480 SYBR Green I Master Kit on a LightCycler480 apparatus (Roche) using standard protocols (40 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... For CHIP-qPCR: qPCR was performed on diluted samples using LightCycler 480 SYBR green 1 master kit (Roche) using primers listed below.
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bioRxiv - Plant Biology 2021Quote: Genomic DNA (5 μg) was used to construct the BS-seq library with the KAPA Library kit (Roche) and EpiTect Bisulfite Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 350 µL of saliva were mixed with 350µL of lysis buffer (MagNA Pure Compact RNA Isolation Kit, Roche). RNA was extracted from 500 μl of sample using a MagNA Pure Compact Instrument (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... TruSeq-style library preparation was carried out using either the KAPA Hyper Prep kit (Kapa Biosystems, Wilmington, MA) or the NEB DNA Ultra 2 kit following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of DNA sample library was done with the use of Kapa HTP Library Preparation Kit (Roche, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: RNA of high quality (RINe>8) was prepared for mRNA-sequencing using the KAPA mRNA HyperPrep kit (Roche). Libraries were quantified using a High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed following the manufacturer’s instructions with the Roche FastStart Universal SYBR Green Master Kit (Roche). The same primers reported above for tp0574 and tprL (but without restriction tags in the latter case ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using ‘Transcriptor High Fidelity cDNA Synthesis Kit’ (Roche Diagnostics, Mannheim, Germany) with oligodT as a primer ...
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bioRxiv - Neuroscience 2020Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany). Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng RNA was reverse transcribed using random hexamers and the Transcriptor First Strand cDNA Synthesis Kit (Roche) following the protocol recommended by the manufacturer ...
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bioRxiv - Genomics 2020Quote: Southern blots were performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon pool was normalized using quantification by qPCR with the KAPA Illumina quantification kit (Roche, Basel, Switzerland). The computational processing of these data was performed using QIIME2 2017.11 (Bolyen et al. ...
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bioRxiv - Genomics 2021Quote: ... The libraries were individually quantified via qPCR using a KAPA Library Quantification Kits (Kapa Biosystems, Wilmington, MA, USA) for Illumina platforms ...
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bioRxiv - Microbiology 2021Quote: ... DNA insertion fragments and backbones were amplified using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Boston, USA), and they were assembled at 50 °C for 1 h.
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bioRxiv - Physiology 2021Quote: ... RNA libraries were prepared using Kapa Stranded RNA-seq kits on samples with a RIN>0.8 according to the manufacturer’s instructions (Roche). Library quantities were determined using QUBIT (ThermoFisher ...
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bioRxiv - Systems Biology 2020Quote: ... We ran 10 μl reactions using the LightCycler 480 platform and the SYBR Green I Master kit (Roche Diagnostics Corporation ...
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bioRxiv - Cancer Biology 2021Quote: The TUNEL staining was performed using an In Situ Cell Death Detection Kit (Roche Diagnostics GmbH, Mannheim, Germany). The paraffin sections were stained with TUNEL reaction mixture for 1 h at 37°C and then mounted using DAPI containing mount medium ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems, K4602) using specific primers for β-actin ...
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bioRxiv - Cancer Biology 2021Quote: RNA-seq libraries for the COH TNBC cohort were generated using the KAPA Stranded mRNA-Seq kit (Roche) according to manufacturer’s instructions and were sequenced on a HiSeq4000 Illumina platform to generate 75 bp paired end reads ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Specific DNA probes for hpt and OsSOG1 were synthesized with a PCR Digoxigenin Probe Synthesis Kit (Roche Diagnostics) using the primers shown in Table S7.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad) ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... and immune complexes were visualized using the ultraView Universal DAB (diaminobenzidine tetrahydrochloride) Detection Kit (Roche cat#760-500). Slides were then washed with a Tris-based-reaction buffer (Roche cat#950-300 ...
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bioRxiv - Microbiology 2022Quote: ... and vRNA were generated by reverse-transcription PCR (RT-PCR) using Transcriptor First Strand cDNA Synthesis Kit (Roche). Primers with specific tags for differentiations of virus mRNA ...
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bioRxiv - Genetics 2022Quote: ... Quantitative real-time PCR (qPCR) was carried out using a KAPA™ SYBR FAST qPCR Kit (KAPA Biosystems) and StepOnePlus™ Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: Southern blot was performed according to DIG High Prime DNA Labelling and Detection Starter Kit II (11585614910, Roche). Briefly ...