Labshake search
Citations for Roche :
3551 - 3600 of 5262 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in 10 µL reactions using KAPA SYBR Fast qPCR Kit (KAPA BIOSYSTEMS). Expression levels were calculated using the 2–ΔΔCt method ...
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bioRxiv - Developmental Biology 2023Quote: ... A DIG-labeled antisense RNA probe targeting lefty1 was synthesized using a DIG Probe Synthesis Kit (Roche). NBT/BCIP/Alkaline phosphatase-stained embryos were dehydrated in methanol before clearing and imaging in 2:1 benzyl benzoate:benzyl alcohol (BBBA ...
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bioRxiv - Microbiology 2023Quote: ... DNA/cDNA libraries with index adapters were then prepared using the KAPA Library Preparation Kit (Roche NimbleGen) and the KAPA Stranded RNA-Seq Library Preparation Kit (Roche NimbleGen ...
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bioRxiv - Microbiology 2023Quote: ... The first targeted PCR was performed using a KAPA HiFi HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland). Thermal cycles were as follows ...
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bioRxiv - Genomics 2023Quote: ... An accurate library quantification of the pool was determined using the Illumina/Universal Kit (KAPA Biosystems, KK4824). The pool was sequenced on an entire lane of a NextSeq 500 HO flow cell to generate paired-end 151 bp reads using the NextSeq 500 High Output Kit v2.5 (300 cycles ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit for RT-PCR (Roche Diagnostics, Basel, Switzerland). qPCR was then performed on a LightCycler™ (Roche Diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (KAPA Stranded mRNA-Seq Kit,KR0960–v5.17, Kapa Biosystems) and the long RNA sequenced (kit as above) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA libraries were generated using either the KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems, MA, USA) and sequenced directly ...
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bioRxiv - Molecular Biology 2023Quote: ... The QUB GTCU generated libraries (3x control and 3x irradiated) using the KAPA mRNA HyperPrep Kit (Roche) prior to paired-end sequencing (2 x 50 bp ...
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bioRxiv - Microbiology 2024Quote: RNA extraction from saliva samples stored in RNAlater was performed with High Pure Viral RNA Kit (Roche), both according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Adapter-ligated fragments were quantified with qPCR using a library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad ...
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bioRxiv - Immunology 2024Quote: Total RNA from T lymphocytes was isolated using the High Pure RNA isolation kit (Roche, catalog 11828665001), quantified in a NanoDrop (ND-1000 ...
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bioRxiv - Immunology 2024Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. #0797014001). Libraries were sequenced at a targeted depth of 25,000 reads per cell using the NovaSeq 6000 S2 100 cycle kit (Illumina).
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bioRxiv - Genetics 2024Quote: ... Libraries were created from entire poly A-selected RNA samples using the KAPA RNA Hyperprep kit (Roche); cleanup steps were performed using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The sequencing library for the Nosed2 samples utilized enzymatic shearing using the KAPA HyperPlus Kit (Roche 07962401001) and 100ng of starting material (SeqCap EZ HyperCap Workflow Version 3.0) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, cat# KK8561). For each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each final pooled library was quantified by qPCR with the KAPA Library Quantification Kit (KAPA Biosystems, USA) and sequenced using the Illumina paired-end protocol on a HiSeq3000 sequencer by the Get-PlaGe core facility (GenoToul platform ...
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bioRxiv - Cell Biology 2024Quote: ... DNA damage was detected using a terminal dUTP nick-end labelling (TUNEL) assay kit (Roche, Cat. # 11684817910). Malondialdehyde (MDA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Adapter-ligated fragments were quantified with qPCR using a library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ligated fragments were amplified for 16 cycles using KAPA Hifi Hot Start PCR kit (Roche, Basel, Switzerland), and purified again with 1X AMPure XP beads ...
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bioRxiv - Biochemistry 2024Quote: ... Deparaffinization was done following a partially modified protocol from the High Pure FFPET DNA Isolation Kit (Roche). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Intact total RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) according to manufacturer’s instructions with random primers ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized with Oligo-dT primer by using Transcriptor High Fidelity cDNA Synthesis Kit (5081963001, Roche). qRT-PCR was performed using Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was purified from 106 infected cells using the High pure PCR template preparation kit (Roche) and subjected to quantitative real-time PCR (qPCR) ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the pooled library was determined using the NGS Library Quantification Complete kit (Roche 07960204001) before being loaded onto a MiSeq sequencer ...
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bioRxiv - Cancer Biology 2024Quote: ... ∼ 2µg of size-selected DNA was used for library preparation using KAPA hyper prep kit (KAPA biosystems) and TruSeq DNA unique dual indexes (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... the libraries were quantified by qPCR using the KAPA library quantification kit for Illumina platforms (Roche, KR0405) using QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
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bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
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bioRxiv - Plant Biology 2024Quote: ... The libraries were then quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (Roche), and their profiles were assessed on an Agilent Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Libraries for April samples were prepared with the DNA Hyper Prep library kit (Kapa Biosystems-Roche, Basel, Switzerland) and libraries for August samples were prepared with the Nextera DNA Flex Library kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Libraries for April samples were prepared with the DNA Hyper Prep library kit (Kapa Biosystems-Roche, Basel, Switzerland) and libraries for August samples were prepared with the Nextera DNA Flex Library kit (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were generated using the Kapa mRNA-Hyper Prep kit with Poly-A Selection (Roche, Basal) and were sequenced on the Illumina NovaSeq (>70 million 2×150 bp sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems, KK8484) with custom Y-shaped adapters ...
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bioRxiv - Cell Biology 2020Quote: ... a DNA library was generated from 300 ng of genomic DNA using Kapa HyperPlus library preparation kit (Roche) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Purified total RNA from pHCEnC was prepared for RNA sequencing libraries using the KAPA mRNA HyperPrep Kit (Roche Sequencing Solutions ...
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bioRxiv - Immunology 2021Quote: ... Each cDNA sample was ten times diluted and amplified using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems) on the CFX Connect™ Real-Time PCR detection System (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-seq libraries were prepared using the KAPA Stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... and the fragmented DNA was input for library construction by the KAPA Hyper Preparation Kit (Kapa Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Sense and antisense RNA probes were transcribed from linearized plasmid DNA using a DIG RNA Labeling Kit (Roche). Sections were prepared by embedding hybridized embryos in gelatin-albumin and sectioning on a vibratome (Leica VT1000S ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The RNASeq libraries were prepared from total RNA using the KAPA Stranded mRNA-Seq Kit for Illumina (Roche) with minor modifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmid DNA was extracted from wild-type strains using Genopure Plasmid Midi Kit (Roche Diagnostics GmbH, Mannheim, Germany). Plasmids were reintroduced by electroporation and their presence was confirmed by PCR assays for gene blaCTX-M and for FII and multiplex (FIA ...
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bioRxiv - Microbiology 2019Quote: ... and was reverse-transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit with random primers (Roche, 04897030001). The relative abundance of HTLV-1 sense-strand transcripts at each time point was measured by qPCR with Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The libraries were quantified by using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824) and sequenced on HiSeq following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... and then diluted for accurate qPCR quantification using KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, MA). The sequencing pool was adjusted to a final concentration of 11.5 pM (for V2 kits ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 ng or less of DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... prior to the preparation of an adapter-ligated library using the KAPA JyperPlus Kit (KAPA Biosystems, Wilmington, MA) according to KAPA-provided protocol ...
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bioRxiv - Microbiology 2020Quote: Real time quantitative RT-PCR was performed with the Light Cycler RNA Master SYBR Green I kit (Roche), following the manufacturer’s instructions ...