Labshake search
Citations for Roche :
3701 - 3750 of 5262 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... A whole genome library was produced for each sample using the KAPA High Throughput library preparation kit (Roche). The standard protocol was followed (Version 5.16) ...
-
bioRxiv - Molecular Biology 2019Quote: Cellular ATP concentrations were determined using the ATP Bioluminescence Assay Kit CLS II (11 699 709 001, Roche) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... the fragmented DNA was converted to a sequence library using a KAPA Hyper Library Preparation Kit (KAPA Biosystems). To analyze transcripts in the HepG2 cells ...
-
bioRxiv - Cell Biology 2019Quote: ... Drug cytotoxicity was evaluated by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Cell Proliferation Kit I, Roche) with a NanoQuant microplate reader (Tecan Trading AG ...
-
bioRxiv - Genomics 2021Quote: ... DNA was prepared using Qiagen Large-Construct Kit and labelled with tetramethyl-rhodamine-5-dUTP (Roche Applied Sciences) by using the Nick Translation Mix (Roche Applied Sciences ...
-
bioRxiv - Genomics 2021Quote: ... DNA was prepared using Qiagen Large-Construct Kit and labelled with tetramethyl-rhodamine-5-dUTP (Roche Applied Sciences) by using the Nick Translation Mix (Roche Applied Sciences ...
-
bioRxiv - Genetics 2020Quote: ... Biochemical analyses were done on a Hitachi-912 Auto Analyzer (Hitachi, Mannheim, Germany) using kits supplied by Roche Diagnostics (Mannheim) ...
-
bioRxiv - Evolutionary Biology 2021Quote: The target-enriched pools were amplified using the KAPA HiFi 2X HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) or NEBNext Q5 HotStart HiFi PCR Master Mix (New England BioLabs ...
-
bioRxiv - Evolutionary Biology 2021Quote: Sheared ligation fragments were end-repaired and A-tailed using the KAPA-Illumina library creation kit (KAPA biosystems) followed by immobilization of mate pair fragments on streptavidin beads (Invitrogen) ...
-
bioRxiv - Physiology 2021Quote: ... Quantitative real-time RT-PCR of target genes were performed using KAPA SYBR FAST qPCR kit (KAPA Biosystems). Expression levels were calculated according to the relative 2−ΔΔCt method.14 All primers are listed (Supplemental Table 2S).
-
bioRxiv - Physiology 2021Quote: ... Quantitative real-time RT-PCR of target genes were performed using KAPA SYBR FAST qPCR kit (KAPA Biosystems). Expression levels were calculated according to the relative 2-ΔΔCt method.65 All primers for target genes are listed (Supplemental Table 5S).
-
bioRxiv - Physiology 2020Quote: ... Synthesis of digoxigenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling Kit (SP6/T7; Roche).
-
bioRxiv - Molecular Biology 2020Quote: Digoxigenin (DIG) or fluorescein labeled RNA probes were made using the DIG RNA labeling kit (Roche, Cat. 11175025910) or fluorescein RNA labeling kit (Roche ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was harvested from cells using a High Pure RNA Isolation Kit (Roche Diagnostics Indianapolis, IN, USA), according to the manufacturer’s protocols ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... the paired-end DNA library was constructed using a KAPA Hyper Prep kit (KAPA Biosystems, Wilmington, MA, USA). The fragmented DNA was end-repaired ...
-
bioRxiv - Neuroscience 2021Quote: ... ATAC-seq library amplification was done using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) and 1.25 μM indexed primers using the following PCR conditions ...
-
bioRxiv - Epidemiology 2021Quote: ... Resulting cDNA was used for quantitative real-time PCR (qPCR) analysis with a SYBR Green PCR kit (Roche). β-Actin was used as reference gene ...
-
bioRxiv - Developmental Biology 2020Quote: ... Digoxigenin (DIG) and fluorescein labeled (FITC) probes were synthesized by using DIG and FITC RNA labelling kit (Roche). Primer sequences are listed in Table S1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared from 250 ng of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to the manufacturer’s specifications ...
-
bioRxiv - Developmental Biology 2021Quote: ... Libraries from 5-10 ng of ChIP DNA was prepared using the KAPA HTP Library Preparation Kit (Roche) by the Next-Generation Sequencing Core at UT Southwestern ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from cells using High pure RNA isolation kit from Roche (Ref. 11828665001, lot. 38800800) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... was used to remove excess antibody and amplified C-circles were detected with CDP-Star® kit (Roche). Membranes were imaged with the Odyssey® Fc Imager (LI-COR ...
-
bioRxiv - Genomics 2020Quote: Pre-capture libraries were prepared with standard library preparation protocols using the KAPA Hyper kit from Kapa Biosystems and then pooled at equal volume and sequenced on the Illumina platform at low depth to determine exact relative abundances ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was extracted from 500 uL of nasal wash fluid using the high Pure RNA Tissue Kit (Roche) and DNA was amplified in the Light Cycler (Roche ...
-
bioRxiv - Microbiology 2021Quote: The RNA was subjected to reverse transcription and second strand synthesis using the cDNA synthesis system kit (Roche). The acid nucleic quantification (RNA and cDNA ...
-
bioRxiv - Immunology 2020Quote: ... 120 ng of RNA were used in the Transcriptor First Strand cDNA synthesis kit (Roche Diagnostics, Laval, QC), and 0.1 μl to 0.2 μl of cDNA generated were analyzed by SYBR Green-based real time PCR (real time-PCR ...
-
bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR reactions were performed with SYBR Green I Master Kit and LightCycler 480 system (Roche). mRNA levels of Col4a1 were normalized to the housekeeping gene Actb or Rpl32 ...
-
bioRxiv - Immunology 2021Quote: ... A pre-amplification step of 9 PCR cycles was performed using the Kapa HiFI Hoststart kit (Kapa Biosystems). The PCR product was purified using AmpureXP beads (Beckman Coulter ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA from LCLs was reverse transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Cat. # 04896866001). RT-qPCR was performed in triplicate using Fast SYBR Green Master Mix (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... followed by cDNA synthesis using 100 ng RNA with the First Strand cDNA synthesis kit (Roche Applied Science) according to manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... Library preparation was performed according to the manufacturer’s protocol using a Kapa Hyper Prep Kit (Kapa Biosystems, KK8501) and barcoded independently with unique dual indexes (IDT for Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: The TUNEL assay was performed in cryosectioned lung tissue using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Libraries (500 ng total RNA) were prepared using the KAPA Hyperprep messenger RNA (mRNA)-Seq Kit (KAPA Biosystems). Purified libraries were normalized by quantitative PCR (qPCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... Total RNA (1 μg per replicate) was used for library preparations using the KAPA RiboErase Kit (HMR) (Roche) following manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... the peptidyl-tRNAs in the PTS buffer were isolated by using a High Pure miRNA Isolation Kit (Roche). After adding 2080 µL of binding buffer ...
-
bioRxiv - Developmental Biology 2022Quote: ... 100 ng of sheared chromatin was end-repaired and A-tailed using the Kapa hyper prep kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Antisense RNA probes were generated using the DIG or DNP based RNA labelling kits from Roche (Merck, Germany). The quality of the RNA probe was checked by agarose gel electrophoresis.
-
bioRxiv - Cancer Biology 2022Quote: One microgram of RNA was subjected to reverse transcription with Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics). qRT-PCR reaction was carried out in 384-well format using 7900HT Fast Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Genomics 2022Quote: ... The concentration in molarity of samples was determined by the KAPA Library Quantification Kit (Kapa Biosystems, cat # 4854). Each replicate was pooled to 4 nM and sequenced separately on an Illumina NextSeq 500 (75-bp paired-end ...
-
bioRxiv - Microbiology 2022Quote: ... was used to detect SARS-CoV-2 mRNAs and visualized by DIG luminescent detection kit (Roche, Indianapolis, IN), according to the manufacturer’s protocol.
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative PCR (qPCR) was performed using the Roche SYBR green I kit on the LightCycler480 machine (Roche Diagnostics) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were then prepared from 3 ng of CUT&RUN DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
-
bioRxiv - Microbiology 2022Quote: ... libraries were prepared from the bound ligation products with the Kapa HyperPrep Kit with Library Amplification Module (Roche) using an on-bead modification to the standard protocol for end repair ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then stained using the in situ TMR red cell death detection kit (Roche Applied Science, cat#: 12156792910) [39] ...
-
bioRxiv - Neuroscience 2023Quote: Cell apoptosis of brain tissues around the hematoma was measured with a TUNEL Apoptosis Assay Kit (Roche, 11684817910) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... PCR was performed according to the manufacturer’s instructions of the KAPA HiFi HotStart PCR Kits (Roche, Basel Switzerland) using barcoded primers and a two-step PCR protocol (NEXTflex™ 16S V4 Amplicon-Seq Kit ...
-
bioRxiv - Microbiology 2023Quote: ... Pre-capture libraries were prepared from up to 100 ng input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we prepared standard genomic libraries using the KAPA Hyper Prep library preparation kit (F. Hoffmann-La Roche AG) and custom-indexes (55) ...
-
bioRxiv - Immunology 2023Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. No. 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cells ...
-
bioRxiv - Genomics 2022Quote: ... The short reads were obtained by preparing Illumina PCR free libraries using the Kapa Hyper Prep Kit (Roche). Briefly ...