Labshake search
Citations for Roche :
3401 - 3450 of 5262 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... LDH release into the supernatants was quantified by colorimetric assay using the Cytotoxicity Detection kit Plus (Roche), which measures the conversion of tetrazolium salt in formazan ...
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bioRxiv - Genetics 2020Quote: ... The concentrations of the pools were measured using the Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the samples were sequenced on the Illumina HiSeq 4000 genome sequencer using 150 bp paired-end SBS chemistry.
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bioRxiv - Physiology 2019Quote: ... Libraries were constructed from purified DNA using the KAPA DNA Library Preparation Kit for Illumina (Kapa Biosystems). Input libraries were prepared from each experimental replicate using 10 ng of DNA purified immediately following shearing ...
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bioRxiv - Genomics 2019Quote: ... perniciosus (embryos, 1st, 2nd and 4th instar, and pupae) using the High Pure RNA Tissue Kit (Roche) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: Poly-A enriched NGS library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) using 50ng of input total RNA according to manufacturer’s protocol using 16 amplification cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 ng of dsDNA was then used as input for the KAPA Hyper Prep Kit (KK8504, Roche) and ligated to KAPA single indexed adapters Set B (KK8702 ...
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bioRxiv - Microbiology 2019Quote: ... 16S rRNA genes were amplified using KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA). Amplification conditions for fast PCR using the KAPA2G™ polymerase were as follows ...
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bioRxiv - Physiology 2019Quote: ... taiwanensis VLB120 was isolated using the High Pure PCR Template Preparation Kit (Hoffmann-La-Roche, Basel, Switzerland). Upstream (TS1 ...
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bioRxiv - Genetics 2019Quote: ... rRNA-depleted RNA was used as input to the KAPA HyperPrep RNA-seq Kit (Kapa Biosystems: KK8540). All libraries were sequenced with 76 base-pair paired-end reads using an Illumina NextSeq (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell-free DNA library was prepared using the KAPA Library Preparation Kit (Kapa Biosystems, Wilmington, USA). The gDNA library was constructed according to our established method [15] ...
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bioRxiv - Synthetic Biology 2020Quote: ... Amplicons were obtained after PCR amplification using Kapa HiFi HotStart Readymix kit from Kapa Biosystems (Cat# KK2602) according to the supplier’s protocol ...
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bioRxiv - Genomics 2020Quote: PI-AnnexinV staining was carried out using the Annexin-V-FLUOS Staining kit (Roche, cat no. 11858777001). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... rRNA-depleted RNA-seq libraries using the KAPA stranded RNA-seq Kit with RiboErase HMR (Kapa Biosystems). All steps were performed according to the manufacturer’s protocol except for the use of custom Illumina-compatible index primers to allow multiplexing ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated cfDNA was first end-repaired and A-tailed using the KAPA HyperPrep kit (Roche, Mannheim, Germany), followed by adapter ligation with index adapters (Integrated DNA technologies) ...
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bioRxiv - Pathology 2021Quote: ... RNAs were reverse transcribed using first strand cDNA synthesis kit for RT-PCR (Roche Diagnostics, Meylan, France), according to the manufacturers’ protocols ...
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bioRxiv - Pathology 2020Quote: ... these protocols were adjusted to a real-time format with Green Essential FastStart Master kit (Roche Diagnostics), in a Nano LightCycler (Roche Diagnostics) ...
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bioRxiv - Pathology 2020Quote: ... cDNA templates were used for real-time quantitative PCR with KAPA SYBR Fast qPCR kit (KAPA Biosystems) and analyzed with the Roche LightCycler 480 ...
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bioRxiv - Microbiology 2021Quote: ... PCR reaction mixtures consisted of 10 μL of KAPA HiFi HotStart ReadyMix 2X PCR Kit (Kapa Biosystems), 0.3 μM forward primer ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA library preparations were performed with the KAPA RNA HyperPrep Kit (KAPA Biosystems, Roche Holding AG) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA library preparations were performed with the KAPA RNA HyperPrep Kit (KAPA Biosystems, Roche Holding AG) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were quantified using Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems Inc.). Average size fragment was determined using a LabChip GX (PerkinElmer Inc. ...
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bioRxiv - Developmental Biology 2019Quote: All the reactions were performed with the KAPA SYBR® FAST qPCR Kit (Kapa Biosystems, MA, USA), which consisted of 0.4 µl of each primer (10 µM) ...
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bioRxiv - Pathology 2021Quote: ... The detection signal was visualized using a DIG-High Prime Detection Starter Kit II as instructed (Roche).
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bioRxiv - Genetics 2019Quote: ... Exact molar concentrations were determined prior to sequencing using the universal KAPA Illumina Library Quantification kit (Roche) on an Applied Biosystems StepOne Plus system.
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bioRxiv - Genetics 2019Quote: ... Real-time PCR (qPCR) reactions were performed using the KAPA SYBR Fast qPCR Kit (Kapa Biosystems; KK4601), with gene-specific primers in technical triplicates and in biological triplicates (Neuro2a cells) ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription with ensuing PCR steps was performed using a Transcriptor One-Step RT-PCR kit (Roche) with EBOV-specific primers ...
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bioRxiv - Plant Biology 2019Quote: ... Sequencing ready libraries were prepared using the Hyper Prep DNA Library preparation kit (Kapa Biosystems, London UK) selecting fragments from 70-350 bp for optimization (see He et al. ...
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bioRxiv - Genetics 2020Quote: Exon 3 of the MSH2 gene was PCR amplified using the Expand High Fidelity PCR kit (Roche) with the following conditions ...
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bioRxiv - Genetics 2020Quote: ... Extracted RNA was reverse transcribed to cDNA with Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Real-time PCR was performed with a LightCycler® 480 II and a LightCycler®480 SYBR Green I Master (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: Both genome and transcriptome libraries were quantified using a next-generation sequencing library qPCR kit (KAPA Biosystems) and run on a LightCycler 480 real-time PCR instrument (Roche) ...
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bioRxiv - Genomics 2021Quote: ... The Illumina paired-end sequencing libraries were prepared using KAPA LTP Library Preparation Kits (Roche Sequencing, USA) with a targeted insert size of ∼550 bp ...
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bioRxiv - Physiology 2021Quote: ... One µg of total RNA was then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche) as previously described [15] ...
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bioRxiv - Physiology 2020Quote: ... triglycerides and PL in each fraction was measured by enzymatic methods using CHOD-PAP kit (Roche Diagnostics) GPO-PAP kit (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries for the UW OvCa cohort were generated using the KAPA mRNA Hyperprep kit (Roche) according to manufacturer’s instructions and were sequenced on a NovaSeq Illumina platform to generate 100 bp paired end reads ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA sequencing libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (HMR; KAPA Biosystems). RNA was fragmented to a size of 100–200 nucleotides and amplified for 11 cycles ...
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bioRxiv - Immunology 2021Quote: ... Stranded mRNA libraries were prepared in the Crick HTS facility using a KAPA mRNA HyperPrep Kit (Roche) and sequenced on the Illumina HiSeq 4000 platform (76bp single-end sequencing).
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bioRxiv - Cancer Biology 2021Quote: ... The ATP synthesis was measured using the luciferin/luciferase ATP bioluminescence assay kit CLSII (Roche, Basel, Switzerland), on a Luminometer (GloMax® 20/20 Luminometer – Promega ...
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bioRxiv - Systems Biology 2021Quote: ... The sequencing libraries were prepared from 500 ng total RNA using the mRNA HyperPrep kit (KAPA BIOSYSTEMS) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... a standardized automated protocol using the OptiView DAB IHC Detection Kit (BenchMark GT automated immunostainer, Ventana, Roche) was performed ...
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bioRxiv - Neuroscience 2021Quote: ... RNA-seq libraries were quantified by qPCR using the KAPA library quantification kit (KAPA Biosystems, Wilmington, MA). Each library was normalized to 2nM and pooled in equimolar concentrations ...
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bioRxiv - Genomics 2022Quote: ... ileum and skin samples using the High Pure RNA Tissue Kit (Cat. No. 12033674001; Roche, Rotkreuz, Switzerland), the concentration and quality of the RNA being measured on aa NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Bromodeoxyuridine (BrdU) and assessed with a BrdU ELISA kit (Roche, Mississauga, ON, Canada) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... library preparation for Illumina sequencing was performed using the KAPA HTP Library Preparation Kit (KAPA Biosystems, KK8234). During library enrichment ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized with primer oligo (dT) using Transcriptor High Fidelity cDNA Synthesis kit (Roche, SUI). PCR amplification was then performed using FastStrat High Fidelity PCR System (Roche ...
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bioRxiv - Immunology 2020Quote: ... Libraries were then pooled at equal molar concentration and quantified (KAPA SYBR® FAST qPCR Kit - Roche)19 ...
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bioRxiv - Genetics 2019Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Genetics 2019Quote: ... for size and by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems) for quantities ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries for RARα ChIP and Input fragments were prepared using the KAPA Hyper Prep Kit (Roche) and submitted for paired-end Illumina sequencing on the NovaSeq 6000 generating 50 bp reads.
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bioRxiv - Genomics 2021Quote: ... the sample pool was quantified using KAPA Library Quantification Kit for Illumina and BioRad qPCR platforms (Roche) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... digoxigenin-labelled probes were synthesised from linear DNA templates using RNA polymerase and digoxigenin-UTP kits (Roche), and purified using SigmaSpin columns (Sigma) ...