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Citations for Roche :
3501 - 3550 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Cells were then harvested and genomic DNA was extracted with a High Pure PCR Template Preparation Kit (Roche 11796828001). MinION runs were performed on R9 with 400-500ng purified DNA per sample following library preparation with Ligation Sequencing Kit 1D (ONT SQK-LSK108) ...
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bioRxiv - Genetics 2020Quote: ... and one day later 2 micrograms of plasmid DNA was transfected into cells using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in GFP trap buffer (50 mM Tris-HCl, 0.5% NP-40 and Roche protease inhibitor cocktail) and the lysate was added to pre-equilibrated GFP trap beads (ChromoTek) ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells transiently transfected with WT EphA2-eYFP were selected with 1.6 mg/ml G-418 solution (Roche; 4727878001) for 12 days to generate a stable cell line ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested 48 hours post transfection and lysed in IP Lysis Buffer (Pierce) supplemented with protease inhibitors (Roche) for 45 minutes at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Neuroscience 2021Quote: ... PFA was removed and cells were washed 3x with PBS then blocked in a buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... Whole cell tissue lysis: Lysis was performed in RIPA buffer supplemented with protease inhibitors (Complete Mini, Roche Applied Science), 0.1 mM PMSF ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were scraped and collected in radioimmunoprecipitation assay buffer RIPA buffer including PhosStop and protease inhibitor cocktail (Roche). Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... Cell pellets were suspended in equilibration buffer (20 mM Tris pH 8.0, 500 mM NaCl) plus protease inhibitors (Roche) and lysed using a high pressure Emulsiflex ...
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bioRxiv - Molecular Biology 2021Quote: (Thawed) Cell pellets were lysed on ice for at least 10 min with 1x protease inhibitors cocktail (PIC; Roche) and 1x quenchers (10 mM NaN3 ...
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bioRxiv - Genomics 2021Quote: ... followed by lysing the cells on ice for 3 min in 50 μl of ATAC-seq RSB containing 0.1% NP40 (Roche), 0.1% Tween-20 (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Microbiology 2022Quote: ... 2013)) were transfected in HeLa cells 24 h before infection using X-tremeGENETM HP DNA Transfection Reagent (Roche, 6366244001), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested and re-suspended in buffer A supplemented with 10 μM ZnCl2 and protease inhibitor (Complete, Roche). Cells were then homogenized by sonication and the crude extract was centrifuged for 30 min at 18,000□×□g ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: Cells were lysed using RIPA lysis and extraction buffer with protease inhibitors and phosphatase inhibitors from Roche (Indianapolis, IN). Protein concentration were determined with the Bio-Rad protein assay (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed twice with ice cold PBS and scraped into cold PBS with phosphatase inhibitors (PhosSTOP, Roche). Pelleted cells were snap-frozen and lysed with 8M urea buffer with 100nM TEAB (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed by passive lysis buffer (25mM Tris-HCl, 150mM NaCl, 0.5% CA630) containing protease inhibitor cocktail (Roche). The cells were lysed in SDS loading buffer and boiled for 5-10min ...
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bioRxiv - Genomics 2022Quote: ... Trim24 knockout TC-1 ES cells (background 129S6/SvEvTac) carrying an RMCE selection cassette (described previously82) were selected under hygromycin (250μg/ml, Roche) for 10 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... ∼600 µL of 0.5 mm diameter glass beads were added and cells were lysed by bead beating on a MagNA Lyser (Roche) three times for 45 s each at 4500 RPM with 2 min rests at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Physiology 2022Quote: ... fixed and proceeded with colorimetric immunoassay for the quantification of cell proliferation according to manufacturer instructions (Roche, Germany, 11647229001).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were subsequently washed with phosphate-buffered saline (PBS) and re-suspended in PBS containing protease inhibitors (Roche, Canada). Cells were heated at 54°C for 3 min in a thermal cycler (VeritiPro ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions of two lung samples from mice of the same sex were pooled and washed with a 2X volume of ACK Lysing Buffer (Quality Biological) as above to lyse red blood cells and then were resuspended in PBS with 0.5% BSA (Roche) and 2 mM EDTA (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Cultured cells were first washed twice with sterile 1X PBS and subsequently scraped from the wells using a cell scratcher in 200µl lysis buffer (RIPA buffer supplemented with 1x Roche complete mini protease inhibitor cocktail and Phospho-Stop phosphatase inhibitor) ...
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bioRxiv - Physiology 2024Quote: ... RNA oligonucleotides were delivered to MLO-Y4 cells using the X-treme Gene siRNA Transfection Reagent (Roche; Cat#04476093001) according to a protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... MIO-M1 cells were transfected with 20 µg/ml of pEGFP or pEGFP-SIK2 vector using FugeneHD (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl+ protease inhibitors (Roche cat # 4693116001) and quantified by BCA assay (Pierce cat # 23223) ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Genetics 2024Quote: ... The testis cells were immediately dissociated through homogenization in 1X PBS containing proteinase inhibitor cocktail (PIC, cOmplete tablets Roche) (PBS+PIC) ...