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Citations for Roche :
3301 - 3350 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were then transfected with the respective plasmids using X- tremeGENE™ HP DNA transfection reagent (Roche, #6365779001) for 48 h (24 h for Toca-1 and Cdc42).
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001, Roche, Switzerland). Chromatin was sheared with Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed using 0.4 mL lysis buffer containing 2x protease inhibitors (Complete, Mini, EDTA-free; Roche), 1x phosphatase inhibitors (PhosStop ...
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bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... glial cell pellets were suspended in 150μl ice-cold Tris buffer (40 mM, pH 7.5) containing protease inhibitors (Roche, Complete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... Cells plated on fibronectin-coated glass coverslips were transfected with PA-TagRFP-emerin using X-tremeGENE HP (Roche). 48-72 hours post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Cells fixed with formaldehyde were permeabilised with 0.1% Triton in PBS and then incubated with blocking reagent (Roche) or 2-5% BSA for 30 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... The protein was produced by transient transfection of HEK 293T cells with XtremeGene™ HP Transfection reagent (Roche) according to the manufacturer’s instructions and purified following a published protocol (77) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNAs or plasmid DNAs using Dharmafect 4 (Dharmacon) or Fugene 6 (Roche Applied Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in cold PBS++ and lysed using 300 μL RIPA buffer containing protease inhibitor (Complete, Roche). Cell lysates (200 μg ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with lysis buffer (final concentration: 50 µg/ml lysozyme, 0.8% NP40, 1X protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Physiology 2021Quote: ... Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Cell Biology 2021Quote: We transfected the FRET reporter into cells by using X-tremeGENE HP DNA Transfection Reagent (Roche, Cat# 6366236001). We mixed 1 μg of plasmid DNA (pECFP-DEVDR-Venus ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were lysed with CST lysis buffer (Cat #9803) that was supplemented with phosphatase (Roche, Cat #04906845001) and protease inhibitors (Roche ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets were afterwards washed in 5 mM Hepes buffer supplemented with EDTA-free protease inhibitors mix (Roche), resuspended in 0.1M DTT ...
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bioRxiv - Molecular Biology 2020Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) with cOmplete™ protease inhibitors (Roche). Tissue lysates were centrifuged at 10,000 × g for 5 min at 4 °C and supernatants were collected for further testing ...
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 genomic RNA was isolated from cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated by incubating with cell lysis buffer (5mM HEPES pH7.9, 85mM KCl, 0.5% NP40, 100uM PMSF, protease inhibitors from Roche) for 10min on ice and centrifuged at 5000rpm for 5min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: The TUNEL staining was performed using an In Situ Cell Death Detection Kit (Roche Diagnostics GmbH, Mannheim, Germany). The paraffin sections were stained with TUNEL reaction mixture for 1 h at 37°C and then mounted using DAPI containing mount medium ...
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bioRxiv - Cell Biology 2021Quote: The plasmids FUG-T2A-GFP-Cre and control GFP were purchased from Addgene #66691 and lentiviral particles were produced in HEK-293T cells using X-tremeGene 9 DNA Transfection Reagent (Roche). Upon ultracentrifugation concentrated virus particles were stored at −80°C and used to infect pancreatic ductal organoid cultures as previously described (Huch et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Stromal vascular fraction (SVF) cells and mature adipocytes were separated from whole adipose tissue biopsies following collagenase (Roche) digestion (1 mg/ml in Hanks’ buffered salt solution ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed on ice in lysis buffer (50 mM AmBic, 0.25% RapiGest, cOmplete protease inhibitor cocktail (Roche)) by sonication (Dr ...
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were dissociated by 10 passages through a glass pipette in the presence of 25U DNase (Roche). Cells were then collected by centrifugation for 5 min at 500xg ...
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bioRxiv - Immunology 2022Quote: To obtain a single cell suspension mouse lungs were digested using collagenase A (10103586001, Roche, 1.5 mg/ml), DNAse I (D4527 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
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bioRxiv - Biophysics 2022Quote: ... We transfected cells with 50 ng of PYL1-TF fusions and reporter-targeting sgRNA using XtremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... Briefly, cells were resuspended in lysis buffer (50mM HEPES pH7.5, 300mM NaCl, 2mM TCEP, + Complete protease inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2022Quote: ... Afterwards samples were incubated for 2 h in the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) enzyme labeling solution at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from cell lysates which were previously lysed by external lysis buffer (Roche, Penzberg, Germany) as described above ...
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bioRxiv - Microbiology 2020Quote: Proteins from various cells were extracted with 1x RIPA buffer containing 1x complete-mini protease inhibitor (Roche; #11836170001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Secreted HBV DNA was extracted according to manufacturer provided protocol (Realtime Ready Cell Lysis Kit, Roche, Mannheim, Germany) and quantified in a qPCR assay (LightCycler® 480 SYBR Green I Master ...
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bioRxiv - Neuroscience 2021Quote: ... we labeled cell nuclei with DAPI (4’,6-diamidino-2-phenylindole; 1:10.000, Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Plasmid and siRNA transfection into HeLa cells were carried out using X-tremeGENE 9 DNA transfection reagent (Roche) and Oligofectamine reagent (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were lysed in 0.25M Sucrose/1xTBS buffer (pH 7.4) supplemented with 1x phosphatase inhibitors (Roche) in a manual glass homogenizer ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with ice-cold phosphate-buffered saline with 1x Protease Inhibitor cocktail (Roche, Cat# 36363600). Millipore ChIP Assay Kit (LOT 3154126 ...
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bioRxiv - Microbiology 2021Quote: RNA was isolated from the neural cultures and VeroE6 cells using the High Pure RNA Isolation Kit (Roche). The concentration of RNA was determined using Nanodrop ...
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bioRxiv - Bioengineering 2021Quote: ... Lactate concentrations in the supernatants and scraped cells were measured using a commercially prepared kit (Roche Diagnostics Corp.). The assay was modified for automated measurement (KoneLab 30).
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bioRxiv - Microbiology 2021Quote: ... cells were harvested and resuspended in PBS supplemented with one complete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 50 mL of lysis buffer ...
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bioRxiv - Immunology 2022Quote: 100 μL overnight culture media of 100.000 cells/200 μL was analyzed on the CEDEX Bio Analyzer (Roche), using the Glutamine V2 Bio or Glucose Bio test kits.
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bioRxiv - Cancer Biology 2019Quote: ... Oocytes were freed from surrounding cumulus cells by addition of 40μl of 4mg/ml collagenase/dispase (Roche, Switzerland), dissolved in DMEM/F12 medium (Mediatech ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was isolated from approximately 1 × 106 cells using the High Pure PCR template preparation kit (Roche) according to manufacturers’ instructions ...