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Citations for Roche :
3601 - 3650 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown for 96 h and cellular viability was determined at indicated time points using the cell proliferation reagent WST1 (Roche) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral preparations were prepared using standard techniques by co-transfection of 293T cells with the lentiviral construct together with pMDLg/pRRE and pMD2.G using Fugene (Roche). Culture supernatants were harvested at 72 h after transfection and concentrated by ultracentrifugation using standard techniques ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged and 25 µL of cell-/ debris-free supernatant was used per well for the LDH assay (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by addition of 5 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (i.e., MTT) using a Cell Proliferation Kit I (Roche Diagnostics, Mannheim, Germany), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Biochemistry 2021Quote: ... or a fixed amount of JR-FL or LAI pseudo-virus (1,000 pg of CA-p24) was added to the cells (70-80% confluency) in the presence of 400 nM saquinavir (Roche) to block secondary rounds of infection and 40 µg/ml DEAE in a total volume of 200 µl ...
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bioRxiv - Developmental Biology 2021Quote: ... The crosslinking reaction was stopped adding Glycine to a final concentration of 0,125 M and the cell pellet was washed three times with cold PBS with protease inhibitors (Complete mini EDTA –free proteinase inhibitor cocktail; Roche). Fixed samples were stored at -80C ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and then washed in PBS buffer before incubation with terminal deoxynucleotide transferase (In Situ Cell Death Detection kit; Roche, Switzerland) for 1 h at 37 °C in a solution containing TMR red dUTP ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were extracted from cells using ice-cold RIPA buffer with complete protease inhibitor mixture (Roche Applied Science, 04693124001). For immunoprecipitation ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell suspensions were prepared by digesting the mouse skin tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells or tissues were extracted on ice using standard CHAPS buffer (60 μL per 106 cells) supplemented with a cocktail of protease inhibitors (Roche) and 20 units of RNase inhibitor (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Molecular Biology 2020Quote: ... 100,000 cells were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to activated ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Genomics 2022Quote: Short-insert paired-end libraries of the lrPCR mtDNA product of the cell lines were prepared with KAPA HyperPrep kit (Roche) with some modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with 27 µl labeling solution plus 3 µl enzyme solution (In Situ Cell Death Detection Kit, AP, Roche) at 37°C overnight ...
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bioRxiv - Genomics 2019Quote: ... An additional Proteinase K incubation (65 μl of 10mg/mL per 7 million cells starting material) at 65°C for two hours was followed by RNase A (Roche; 15 μl of 10mg/mL per 7 million cells starting material ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in 500 μl lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.3% NP40, 1× Roche Complete) and kept on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... PBS was aspirated and the cells where lysed in lysis buffer (3x radioimmunoprecipation assay buffer, protease inhibitor cocktail (Roche 1169749801), 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Molecular Biology 2019Quote: Detection of DNA strand breaks in MC3935-treated schistosomula was performed using the In situ Cell Death Detection kit (Roche), as previously described (33) ...
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bioRxiv - Cell Biology 2020Quote: ... washing cells twice with 2mL of cold PBS and lysing in 1mL RIPA buffer with protease inhibitor cocktail (Roche, 4693159001). Collected media was spun down at 400x g for 5 minutes to pellet any floating cells ...
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bioRxiv - Developmental Biology 2019Quote: Embryos were injected with ndr2 RNA (and MOs) at one-cell stage as described above and dechorionated using Pronase (Roche). At 256- to 512-cell stage ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed twice in ice cold PBS and cell pellets were lysed for 10 min at a density of 107 cells/ml in lysis buffer supplemented with Protease inhibitor (Complete Mini EDTA-free Protease Inhibitor cocktail tablets; Roche). Lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The infected cells were harvested in RIPA buffer solution supplemented with cOmplete™ and EDTA-free Protease Inhibitor Cocktail (Roche). Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: Protein lysates from adult zebrafish tissues and transfected HEK293T cells were prepared using RIPA lysing buffer with EDTA-free protease inhibitor (Roche). Concentration of protein lysates was determined using either the Pierce BCA protein assay kit (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... The cytotoxic potential of the samples was determined from THP1-cell supernatants after 3 hours by using the Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and co-transfected into 60-70% confluent BHK cells grown in 6-well plate tissue culture plates using Fugene HD transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells became 70-80% confluent and were transfected with the corresponding plasmids using the transfection reagent FuGENE 6 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: PFA fixed zebrafish larvae were used in combination with the In situ Cell Death Detection Kit (TMR and Fluorescein) (Roche). After O/N fixation larvae were washed and permeabilized for 45 min at 37°C using Proteinase K ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... subtilis strains at the different time points were evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) using the In-Situ Cell Death Detection Kit with fluorescein (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The cDNA encoding for the A33 construct was obtained by total RNA extraction from the HT-29 colorectal carcinoma cell line (High Pure RNA Isolation Kit, Roche), reverse transcription (Transcriptor reverse transcriptase ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 200 mM NaCl, Roche protease inhibitor cocktail) and lysed by two passes through an EmulsiFlex-C5 homogenizer (Aventis) ...
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bioRxiv - Cell Biology 2019Quote: ... TUNEL staining was performed on testis sections according to the instructions provided with the cell death detection kit (11684795910, Roche). To reduce experimental variations ...
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bioRxiv - Microbiology 2021Quote: ... each tube contained about 106 of cells in 200 μl of the digestion buffer containing 50 μU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... Transfected HEK29T cells and COS-7 cells were washed and lysed with 1% Triton X-100 in PBS with protease inhibitor cocktail (Roche) at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with the TUNEL reaction mixture according to the protocol (In situ Cell Death detection Kit, TMR red; Roche) and incubated for 1 h at 37°C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...