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Citations for Roche :
3401 - 3450 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed and lysed with Lysis Buffer in the presence of 1X Protease Inhibitor Cocktail (Roche,11873580001). Chromatin was fragmented into small pieces (200-500 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the apoptosis of MIN6 cells in each group was assessed using TUNEL apoptosis detection kit (Roche, Basel, Switzerland). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... or MDCK-Ecad Res cells were pre-treated with Mitomycin C at a concentration of 10μg/ml (Roche) for a duration of 1 hour to inhibit cell proliferation ...
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bioRxiv - Microbiology 2023Quote: ... Upon reaching OD600= 0.3 total RNA was extracted from cells using a High Pure RNA Isolation Kit (Roche). Samples at different time points were centrifuged for 5 min at 2,000 relative centrifugal force and pellets were flash-frozen in liquid nitrogen ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 20 mM Tris-HCl pH 8 containing a EDTA-free protease inhibitor cocktail (Roche). For the purification of protein samples that had to be analyzed both by reducing and non-reducing SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were stained using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL apoptosis assay kit, Roche, American), and the cells were viewed under a fluorescence microscope.
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bioRxiv - Genomics 2022Quote: Crosslinked cells were incubated in Cell Lysis Buffer (10 mM HEPES pH 7.5, 10 mM NaCl, 0.2% IGEPAL) + Protease Inhibitors (Roche) for 5 minutes on ice ...
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bioRxiv - Genomics 2022Quote: ... Cells were collected in 1xPBS supplemented with 1X complete EDTA-free protease inhibitor cocktail (PI tablets, 5056489001, Roche) on ice ...
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bioRxiv - Pathology 2023Quote: ... The slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Cat. # 950-500, Roche Diagnostics) for antigen retrieval ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells were transiently transfected using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Cat. # XTG9-R0) with 6 µL of transfection reagent to 2 µg plasmid for 48 hr ...
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bioRxiv - Immunology 2023Quote: Cells were isolated from the brain by mechanical disruption followed by collagenase D digestion (Roche Pharmaceuticals, Mannheim, Germany). The resulting cell suspension was resuspended in 40% Percoll (GE Healthcare Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The assay was performed using the In Situ Cell Death Detection (TUNEL) Kit with TMR Red (Roche, 12156792910) as previously described13 ...
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bioRxiv - Microbiology 2023Quote: The RNA of all cell culture supernatants was extracted using High Pure Viral RNA kit (Roche, REF.11858882001). Viral RNA quantification in each sample was performed by quantitative reverse transcription PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed three times (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... and cells were washed and scraped into ice cold PBS containing Complete EDTA-free Protease Inhibitor Cocktail (Roche). Cells were pelleted in aliquots of 3.6*107 cells ...
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bioRxiv - Physiology 2023Quote: ... the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) in situ cell death detection kit (Roche, Basel, Switzerland) was used according to the manufacturer’s instructions (including incubation for 1 h at 37 °C) ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were lysed in 1 ml of mild lysis buffer containing protease inhibitor mixture (Roche Life Sciences) and phosphatase inhibitor mixture (EMD Millipore) ...
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bioRxiv - Bioengineering 2022Quote: Cultured cells were harvested and lysed in ice-cold RIPA buffer with cOmplete Protease Inhibitor Cocktail (Roche 11697498001). Lysates were mixed with Laemmli buffer containing β-mercaptoethanol and boiled at 70 °C for 10 min to prepare SDS-PAGE samples ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue slides were heat pre-treated using a Cell Conditioning Buffer 1 (pH 8) (Roche Diagnostic, Meylan, France) at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 1 µg/mL plasmid DNA using Fugene HD transfection reagent (Roche Diagnostics, Indianapolis, IN).
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bioRxiv - Developmental Biology 2023Quote: Human embryo sorted cells were collected in Eppendorf containing 6µl of PBS supplemented with 0.5µl of Protector RNAse inhibitor (Roche, 3335399001) and conserved in −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Neuroscience 2023Quote: ... Vectors were co-transfected with packaging-defective helper plasmids into 293T cells using Fugene 6 transfection reagent (Roche). Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspension was then chemically digested by incubation at 37°C for 30 min with collagenase (Roche #11088866001), dispase (Stemcell Technologies #07923 ...
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bioRxiv - Neuroscience 2023Quote: TUNEL staining was performed for paraffin sections using the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Cell death was assessed in tissue sections using terminal deoxynucleotidyl transferase-mediated dUTP nick- end labeling (TUNEL, Roche TUNEL kit (12156792910 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 min) and the cell pellet was resuspended in 1 ml vPBS containing EDTA-free protease inhibitor (Roche). The cells were pelleted again by centrifugation (750 x g ...
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bioRxiv - Cell Biology 2024Quote: ... and the fixed cells were then resuspended in 1 ml RT vPBS containing EDTA-free protease inhibitor (Roche). The cells were attached to the coverslips by centrifugation (1000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... 18S and 28S rRNA samples purified from HeLa cell extract were reverse transcribed using transcriptor reverse transcriptase (Roche) and amplified by PCR using Pfusion PCR polymerase (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing chromatin fragments was then treated with 6-10 µg/g cells DNase-free RNase (Roche) at 37°C for 30 minutes and subsequently cleared by centrifugation at 30,000 rcf for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... whole cell lysates were prepared using 2x Laemmli buffer containing phosSTOP and protease inhibitor cocktail (Roche, Mississauga, ON). Lysates were passed through a 27-gauge needle ten times ...
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bioRxiv - Genetics 2023Quote: ... 48 hrs after transfection, the cells were washed with DPBS and lysed (1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial cell wall was mechanically lysed in Lysing Matrix B using a MagNA Lyser (Roche, Basel, Switzerland). After centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with cold PBS and lysed with a SDS lysis buffer supplemented with protease inhibitor (Roche). Crosslinked clinical samples were washed thrice with cold PBS ...
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bioRxiv - Cell Biology 2024Quote: Cultured cells were lysed on ice through repeated pipetting in 2% SDS supplemented with Protease Inhibitor Cocktail (Roche) and PhosStop phosphatase inhibitor (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... Cell lysates were prepared using RIPA lysis buffer supplemented with phosphatase and protease inhibitor cocktail (Roche, Basel, Switzerland), followed by centrifugation at 13,000g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... followed by a subsequent incubation at 95 °C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics, Canada). Following this ...
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bioRxiv - Systems Biology 2024Quote: Paraffin-embedded tissue sections were processed with an in-situ cell-death detection kit (11684795910; Roche, Basel, Switzerland) for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in lysis buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... culture media were collected and cells harvested on ice in Nonidet lysis buffer supplemented with protease inhibitor mixture (Roche). Culture media and cell lysates were precleared and α1AT was immunoprecipitated with a α1AT polyclonal antibody (total ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from sorted cells by first decrosslinking cells in a solution of 10 mM Tris pH 7.5 and 10 mg/mL Proteinase K (Roche) in PBS at 55°C for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI (50 mM Tris, 100 mM NaCl, pH 8, plus Complete Protease Inhibitor Cocktail, Roche) and broken using 0.1 mm silica spheres (Lysing Matrix B ...