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Citations for Roche :
301 - 350 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Bacteriological peptone (cat. LP0037) and yeast extract (cat. LP0021) were purchased from Oxoid. Protease inhibitor cocktail (cat. 70221) was purchased from Roche. 3-hydroxynaphthalene-2,7-disulfonic acid disodium salt (2-naphtol-3,6-disulfonic acid disodium salt ...
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bioRxiv - Cell Biology 2020Quote: ... colcemid (Roche cat #10295892001) was added to cell growth media to yield final concentrations ranging from 7 to 200 nM 5 hours before fixation and immunostaining ...
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bioRxiv - Genetics 2020Quote: ... one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche, cat. # 11836170001) in 15 mL conical tubes ...
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bioRxiv - Cell Biology 2021Quote: ... PhosSTOP (Roche, cat # 4906837001), and protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... ATPγS (Roche, Cat#11162306001), MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... (Roche Diagnostics, Cat#: KK8504). Libraries were sequenced on HiSeq2500 with paired-end reads ...
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bioRxiv - Developmental Biology 2020Quote: ... Neural stem / progenitor cells were seeded in coated 24-well plates (1.6×105 cells per well) and transfected with the reporter plasmids using xTremeGENE HP DNA transfection reagent (Roche). Cells were collected 48hr after transfection using Passive lysis buffer (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... The mixture of plasmids (16.0 μg in 50 μL ddH2O) was added to 48 μL of X-tremegene 9 DNA transfection reagents (Roche) that pre-incubated for 5 min in 750 μL OptiMEM (Gibco) ...
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bioRxiv - Biochemistry 2019Quote: ... The Bacmid DNA was purified and transfected into SF9 insect cells with Xtreme gene 9 DNA transfection reagent (Roche). The first virus generation ...
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bioRxiv - Biophysics 2020Quote: ... N2A cells were transfected with 1.0 μg of a cDNA construct and 2 μL of X-tremeGENE HP DNA transfection reagent (Roche Diagnostics GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids and ssDNA were transfected into hESC_H1 (WiCell) of 70-85% confluency using the XtremeGENE 9 transfection reagent (Roche). After 48 hours ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with mammalian construct DNA according to manufacturer instructions with X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Cancer Biology 2021Quote: U2OS cells stably expressing T192-Flag-FKBP were grown on coverslips and transiently transfected with either MYOF-FRB* or MYOFΔC2-FRB* using X-tremegene 9 DNA Transfection Reagent (Roche). After 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... DF1 cells were transfected with the indicated RCAS plasmid using X-tremeGENE 9 DNA transfection reagent (Roche, Catalog# 06365809001) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Virus stocks were generated by transfection of 293T cells with each plasmid clone of HSIV-vif using Fugene 6 or X-tremeGENE 9 DNA transfection reagent according to the manufacturer’s protocol (Roche). Infectious titers were determined by limiting dilution infection analysis using TZM-bl indicator cells and the amount of virus in supernatants was measured by HIV-1 p24gag antigen ELISA (Advanced Bioscience Laboratories).
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bioRxiv - Immunology 2019Quote: ... 4μg of Env DNA containing CMV promoter was combined with 4μg of SG3Δenv backbone and FuGene 6 transfection reagent (Roche Diagnostics) was added as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 million S2 cells were transfected with 2 μg of plasmid using Xtreme-GENE HP DNA transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... were transfected with 0.5μg pRRL PKACa WT or pRRL PKACaM120G and 1.5 μg of indicated candidate substrate using X-tremeGENE HP DNA transfection reagent (Roche). 30 hours after transfection ...
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bioRxiv - Genetics 2020Quote: ... and one day later 2 micrograms of plasmid DNA was transfected into cells using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested 48 hours post transfection and lysed in IP Lysis Buffer (Pierce) supplemented with protease inhibitors (Roche) for 45 minutes at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... 4 million cells were seeded in a 10 cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell extracts were prepared after transfection and incubated with indicated antibodies together with Protein A/G beads (Roche) overnight ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: HEK293T cells were washed with 1xPBS 48 hours post transfection and were lysed in 200μl radioimmunoprecipitation assay (RIPA) buffer (ThermoScientific) with 1X cOmplete Protease Inhibitors (Roche) for 15 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were cotransfected with 4 μg of Env-deficient HIV-1 proviral plasmid (Q23ΔEnvGFP) and 2 μg of HIV-1 Env clone of interest using Fugene 6 transfection reagent (Roche) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The bacmid produced by DH10Bac cells was transfected into Sf9 cells using X-tremeGENE HP DNA Transfection Reagent (Roche), and then cultured at 27 °C ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatant containing retroviruses was harvested after 48 hours and premixed with DOTAP liposomal transfection reagent (Roche, Basel, Switzerland) prior to spin transfection at 2400 rpm at 32°C for 2 hours into target cells ...
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bioRxiv - Physiology 2020Quote: ... Cells were plated on six-well culture plates at 50% confluency and then transfected with 1 μg/well of the expression plasmid (pCDNA3.1-hTau441) using X-tremeGENE9 DNA transfection reagent (Roche). Cells were transferred to a 10-cm dish containing 300 μg/ml gentamycin-disulfate (G418 ...
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bioRxiv - Microbiology 2022Quote: ... 2013)) were transfected in HeLa cells 24 h before infection using X-tremeGENETM HP DNA Transfection Reagent (Roche, 6366244001), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Lysis was performed 24 hours from transfection in lysis buffer (TBS, 0.4% Triton X100) supplemented with 1X protease inhibitor cocktail (catalog #11697498001; Roche) and phosphatase inhibitor cocktail (catalog #04986837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 cells were transfected with FAM-labelled hairpins using X-tremeGENE 9 DNA Transfection Reagent (Roche, USA; 1.0 µL). After 16 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid minigenes were transfected 24 h after cell seeding using X-tremeGENE 9 DNA Transfection Reagent (Roche; Mannheim, Germany). HepG2 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μg pAcBAC3 plasmid was used to transfect RAW 264.7 cells by mixing the DNA with 6 μg X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were detached 24 h after transfection with Trypsin-EDTA (0.25% wt/vol) and re-plated onto 10 µg/ml fibronectin (Roche) pre-coated MatTek dishes with glass bottoms ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected using 90ng of each corresponding vector diluted in 10ul of opti-medium and 0.3ul of X-treme GENE HP DNA Transfection Reagent (Roche). 10ng of Renilla vector (Promega ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated by co-transfecting HEK293T cells with the lentiviral construct pCMV-dR8.91 (Δ8.9) plasmid and the pMDG envelope-expressing plasmid using X-tremeGENE 9 DNA Transfection Reagent (Roche, 6365779001). Viral supernatant was collected within 24-48 h post-transfection for subsequent infection of the target cells.
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Biochemistry 2023Quote: ... cells were co-transfected with 0.5 µg HSD11B1 and 0.5 µg H6PDH DNA using X-tremeGENE™ HP DNA transfection reagent (Roche) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... dishes were co-transfected with either 0.5 µg HSD11B1 and 0.5 µg H6PDH or 0.5 µg AKR1C3 and 0.5 µg pCINeo using X-tremeGENE™ HP DNA transfection reagent (Roche). Additional dishes were transfected with 1 µg of the pCINeo (no insert ...
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bioRxiv - Genomics 2020Quote: A screenshot showing read visualization by IGV of the region were the Alu insertion occurred in the positive control sample (BRCA1) when using the SeqCap EZ Exome v3.0 kit from Roche for exome capture is presented in Figure 2 ...
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bioRxiv - Microbiology 2022Quote: ... Library quality control and quantification were performed using a KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems, KK4873) and the 2100 Bioanalyzer equipped with a High Sensitivity DNA kit (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... CAD5 cells were incubated with (0.01%) non-infectious brain homogenate (10% w/v in 0.32M sucrose) to control for efficient proteinase K (PK) (Roche) digestion and to compute the background of the assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Library quality control and quantification were performed using a KAPA Library Quantification kit for Illumina platforms (Kapa Biosystems, KK4873) and a 2100 Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral preparations were prepared using standard techniques by co-transfection of 293T cells with the lentiviral construct together with pMDLg/pRRE and pMD2.G using Fugene (Roche). Culture supernatants were harvested at 72 h after transfection and concentrated by ultracentrifugation using standard techniques ...
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bioRxiv - Cell Biology 2020Quote: ... were co-transfected with pLentiCRISPRv2 containing the indicated sgRNA sequences or pCW57 with the indicated cDNAs with XTremeGene 9 Transfection Reagent (Roche). After 12 hours ...
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bioRxiv - Immunology 2021Quote: ... 1.0 × 106 THP-1 cells were transfected with mixture of two sgRNAs-expressing plasmids (0.5 µg each) using X-tremeGENE™ 9 DNA Transfection Reagent (6365779001, Roche) according to manufacturer’s manual ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-CAA GTG CAA CAG TTT CTC ATT-3’/5’-TGT TTG ACT ACA CTC ACA CT-3’) using X-tremeGENE 9 DNA Transfection Reagent (Roche). 48 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... and the next day a total of 1 μg per well of a 1:1 mix of X22B and X22C CRISPR plasmids (either pX459 or pX462) was transfected by use of X-tremeGENE HP DNA transfection reagent (Roche). The next day ...
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bioRxiv - Cell Biology 2019Quote: HEK293TN cells transfected as described above with the indicated constructs were harvested 48 hours post-transfection by lysis in RIPA buffer supplemented with protease inhibitors (Roche) or in passive lysis buffer (Promega) ...