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Citations for Roche :
451 - 500 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and PhosphoSTOP (Cat#04906837001, Roche). The protein concentration in the lysates was determined using the Bio-Rad Protein Assay Kit – BSA (Cat#5000002 ...
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bioRxiv - Genetics 2023Quote: ... 2nd Generation (Roche, cat#03353621001) for cDNA production paired to Phusion High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosSTOP (Cat. #04906837001, Roche) and resolved by 4-15% gradient SDS-PAGE (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 0.2% Triton (Roche, Cat# 11332481001). The blocking buffer was then removed ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosSTOP (Cat. #04906837001, Roche). The following antibodies were used for immunoblotting ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or DAPI (Roche, Cat. #236276) were used as counterstains for DCX and GFP immunolabeling ...
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bioRxiv - Immunology 2023Quote: ... protease inhibitor (Roche, cat#4693132001), and PMSF (CST ...
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bioRxiv - Cell Biology 2022Quote: ... and PhosStop (Roche. Cat# 4906845001).
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bioRxiv - Cancer Biology 2024Quote: ... and PhoSTOP (Roche, Cat# 04906837001). Protein concentration was determined using Pierce 660 nM Protein Assay Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... ERG (Roche, Cat. No 6478450001) undiluted with 12 minutes of incubation ...
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bioRxiv - Genetics 2024Quote: ... 1X PhosSTOP (Roche Cat. # 04906837001), 1 mM NaF ...
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bioRxiv - Immunology 2024Quote: ... DNAse I (Roche, Cat#04416728001) 10 ug/ml and 1.5 mM Calcium Chloride ...
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bioRxiv - Neuroscience 2023Quote: ... and phosphatase (Roche, Cat. # 04906837001) inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... targeting the indicated genes (Table S2) at a final concentration of 50 nM or 100 nM using X-tremeGene siRNA transfection reagent (Roche, 04476093001) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Twenty-four hours post-transfection cells were rinsed with PBS and lysed in NP40 buffer supplemented with cOmplete™ protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation and incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
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bioRxiv - Biochemistry 2022Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 µg of 330-BFP-CYP3A4-enhancer-R-sgRNA were transfected with X-tremeGENE 9 DNA transfection reagent (Roche 6365809001). After 3-5 days ...
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bioRxiv - Immunology 2022Quote: ... Phoenix-Amphoteric packaging cells were transfected with MP71 constructs in combination with pVSV using FUGENE HD transfection reagent (Roche Molecular Biochemical) to obtain retroviral supernatants ...
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bioRxiv - Cancer Biology 2022Quote: ... expanded to passage 4 and transfected with RCAS-PDGFB-HA or RCAS-shp53-RFP using a Fugene 6 Transfection kit (Roche, 11814443001). Cells were cultured with DMEM media (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... were transfected with 10 µg pASW plasmids carrying plant DCL3 family genes (pASW-AtDCL3, OsDCL3 or OsDCL5) with 20 µl X-tremeGENE™ HP DNA Transfection Reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Biochemistry 2021Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells transfected with a combination of RCAS plasmids (RCAS PDGFB-HA, RCAS shp53-RFP) using the FuGENE 6 transfection kit (Roche, 11814443001) according to the manufacturer’s protocol and as previously described 86 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmids were transfected into cultured cells susceptible to the original viruses using X-tremeGENE™ HP DNA transfection reagent (Roche). For BmNPV ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 ul of purified bacmid were resuspended in 130 ul Sf-900 II media with 10 ul X-treme XP transfection reagent (Roche # 06366236001) and incubated at room temperature for 15 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were lysed 72 hours post transfection or post (first) transduction using the DirectPCR lysis reagent (PeqLab) supplemented with 200 μg/ml proteinase K (Roche Diagnostics). The targeted genomic locus was then PCR amplified with primers flanking the expected cutting site (Supplementary Table 3 ...
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bioRxiv - Genetics 2021Quote: ... and the internal control vector TK (25 ng per well for 48-well plate) were co-transfected into the cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). The transfected HEK293T cells were harvested in 65 μL passive lysis buffer (Promega ...
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bioRxiv - Genomics 2021Quote: ... 3.75 μg of psPAX2 and 1.25 μg of pVSVG) were transfected into LentiX-293T cells using 20 μl of X-tremeGENE™ HP DNA Transfection Reagent (Roche) at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). Virus-containing supernatant was collected 48 hours after transfected and passed through a 0.45μm filter ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA expressing vectors along with lentiviral packaging vectors Delta-VPR and VSV-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001) ...
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bioRxiv - Cell Biology 2022Quote: ... IFNγ-R2-eGFP plasmid was transfected in HeLa or Caco-2 cells with X-tremeGENE HP DNA transfection reagent (Roche, 6366236001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant plasmid pcDNA3.1-Orm2 was transiently transfected into LO2 cells for 48 h with X-treme GENETMHP DNA Transfection Reagent (Roche, Basel, Switzerland).
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant baculoviruses were generated by transfecting 2.5 μg of a transfer bacmid into Sf9 cells (2.5 mL at a density of 106 cells/mL) using 3 μL of X-tremeGENE™ HP DNA Transfection Reagent (Roche) and 100 μL Transfection Medium (Expression Systems) ...
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bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
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bioRxiv - Genomics 2023Quote: ... sgRNA- or cDNA-expressing vectors and lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293T cells using XTremeGene 9 transfection reagent (Roche #636478700). The supernatant containing virus was collected 48 h after transfection and passed through a 0.45-µm filter ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed on six-well plates or confocal dishes for different purposes with plasmids using X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Five mice were not treated and kept as controls and twenty-seven mice were treated orally with Capecitabine (Xeloda; Roche Laboratories) at a dose of 540 mg/kg ...
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bioRxiv - Zoology 2019Quote: cDNA (1 μL) from control larvae was amplified using the KAPA HiFi HotStart DNA Polymerase Taq (Kapa Biosystems, Wilmington, MA, USA), insect-HSP70 degenerate primers (from Bernabò et al. ...
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bioRxiv - Genomics 2021Quote: ... Sample cDNA and library quality controls were performed using the Agilent 4200 TapeStation instrument and quantified by qPCR (Kapa Biosystems/Roche). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Sample cDNA and library quality controls were performed using the Agilent 4200 TapeStation instrument and quantified by qPCR (Kapa Biosystems/Roche). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Library quality control was performed with an Agilent 2100 Bioanalyzer and quantity determined via the KAPA Library Quantification Kit (KAPA Biosystems).
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bioRxiv - Molecular Biology 2024Quote: ... Library quality control was performed with an Agilent 2100 Bioanalyzer and quantity determined via the KAPA Library Quantification Kit (KAPA Biosystems). WGBS libraries were then pooled to meet the required 1µg DNA input necessary for targeted enrichment ...
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bioRxiv - Cell Biology 2024Quote: ... Sample cDNA and library quality controls were performed using the Agilent 4200 TapeStation instrument and quantified by qPCR (Kapa Biosystems/Roche). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Sample cDNA and library quality controls were performed using the Agilent 4200 TapeStation instrument and quantified by qPCR (Kapa Biosystems/Roche). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM KCl) supplemented with protease and phosphatase inhibitor cocktails (Roche, cat#05892791001 and cat#04906837001), was used to homogenize brain tissue as described previously 55 ...