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Citations for Roche :
201 - 250 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Plasmids were incubated for 20 min with a mix of XtremeGENE9 transfection reagent (Roche) and OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... HepG2 cells were transfected with PX459-p62 with X-tremeGENE HP transfection reagent (Roche). Twenty-four h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 0.525 µg VSV-G and 31.5 µL X-tremeGENE HP DNA Transfection Reagent (Roche). All components were premixed and equilibrated for 15 min at 25 °C before being transferred in a dropwise manner into HEK 293 T cells grown in a 10-cm dish with 10 mL DMEM and 10 vol % fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293T cells were transfected using X-tremeGENE™ HP DNA Transfection Reagent (Roche, 6366244001) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: All used chemicals were of the purest grade available and were from Sigma–Aldrich with the exception of: X-tremeGENE™ HP DNA Transfection Reagent (Roche, Switzerland), Transfection Medium (Expression Systems ...
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bioRxiv - Cell Biology 2023Quote: ... containing 5 µl of X-tremeGENE 9 transfection reagent (XTG9-RO Roche, Sigma-Aldrich). Three days after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... DNA vectors were transfected using FuGENE 6 transfection reagent (Roche Applied Science, Laval, QC) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into U2OS cells using JetPRIME transfection reagent (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... including all recommended controls (LDH; Cytotoxicity Detection Kit Plus, Roche Applied Sciences, Penzberg, Germany). Cell-free culture medium was measured as background ...
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bioRxiv - Synthetic Biology 2022Quote: ... and quality control was performed via fragment analyzer (BioAnalyzer) and qPCR (Roche Lightcycler 480). The final library pool was normalized ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with siRNA oligonucleotides using X-treme GENE 9 DNA Transfection reagents (Roche) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Transfection of plasmids for overexpression purposes was performed using X-tremeGENE™ HP (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with constructs using the X-tremeGENE HP DNA transfection reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 150-300 ng of indicated construct using XtremeGene 9 transfection reagent (Roche) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were plated on 25-mm diameter coverslips and transfected using Fugene6 transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 µg DNA construct and 1 µL X-tremeGENE DNA Transfection Reagent (Roche, Hertfordshire, UK) were diluted in 50 µL OPTIMEM serum-free media (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 µg sgRNA plasmid using X-tremeGENE™ 9 DNA transfection reagent (Roche 06365809001). One day after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and pMDLg/pRRE (500 ng each) into HEK293Ts using X-tremegene HP Transfection reagent (Roche). Lentivirus was harvested 72 hours later and subsequently used to infect JR20s supplemented with 4 μg/ml Polybrene ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 72 hours post-transfection genomic DNA was isolated with High Pure Template Preparation Kit (Roche). INDEL occurrence was assessed by T7 Endonuclease I assay on PCR-amplified DNA fragments surrounding the target DSB.
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bioRxiv - Developmental Biology 2020Quote: ... Plasmid DNA was transfected to HEK293T cells using X-tremeGENE HP DNA transfection Reagent (Roche). Transfected cells were harvested at 48◻hours after transfection for further analysis.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T and CHO-K1 cells were transfected with the FuGENE6 transfection reagent (Roche Applied Bioscience) and selected by the addition of puromycin (1 μg/ml and 10 μg/ml respectively ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were introduced into HEK293T cells using the FuGENE 6 transfection reagent (Roche Applied Science). Cells were selected with puromycin (1 μg/ml) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μg of plasmid and 4 μl of X-tremeGENE HP DNA Transfection Reagent (Roche) were used per well ...
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bioRxiv - Cell Biology 2023Quote: ... Both plasmids were transfected into HeLa cells using the X-tremeGENE9 DNA Transfection Reagent (Roche) and selected in medium containing G418 (1 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... Nup96-GFP KI U2OS cells were transfected using X-tremeGENETM 9 DNA Transfection Reagent (Roche), and 24h after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene #12259 into HEK-293T cells using X-tremeGENE HP DNA transfection reagent (#06366236001; Roche). Lentiviral supernatants were applied to cells for 48h in the presence of polybrene (10 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmids were transiently transfected into HeLa cells using Fugene HD transfection reagent (Roche, Germany) for 48 hours to express the corresponding E2F-EGFP fusion proteins ...
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Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulationbioRxiv - Biophysics 2023Quote: ... and after 24h they were transiently transfected using X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Cancer Biology 2024Quote: Lenti-vectors were prepared in 293T cells using X-tremeGene HP DNA transfection reagent (Roche), with psPAX2 and pMD2.G (Addgene ...
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bioRxiv - Genetics 2021Quote: ... The reference/control sample libraries were amplified with the KAPA HiFi HotStart ReadyMix (Roche Sequencing). Library amplification was completed following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... A control homogenated with PBS and protease inhibitor (PI) cocktail (complete EDTA-free tablet, Roche) was included to assess the role of endogenous proteases ...
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bioRxiv - Molecular Biology 2024Quote: ... and the CAT ELISA kit (Roche, cat. # 11758241001). The ratios of CAT/βGAL were then analyzed to calculate the relative IRES activity.
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bioRxiv - Immunology 2021Quote: ... Viral plasmids were transduced in HEK-293T cells using X-tremeGENE HP DNA transfection reagent (Roche). After the addition of X-tremeGENE HP to the viral plasmids the solution was mixed in 100 µL of DMEM medium without serum or antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... we operated according to the protocol of X-tremeGENE HP DNA Transfection Reagent (Roche, Basel, Switzerland). After transfection ...
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bioRxiv - Cancer Biology 2020Quote: Transient transfection of plasmids for overexpression purposes was performed using X-tremeGENE™ HP (Roche Diagnostics) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of each plasmid was combined with Fugene transfection reagent (Roche Applied Science, Mannheim, Germany) at a ratio of 5 µl of transfection reagent to 1 µg DNA in 500 µl of MEM and incubated at room temperature for 30 minutes prior to addition to a 10 cm dish of HEK293 cells at 50% confluence ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with these mixtures using 2.4μL of X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were transfected into cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001). Then the transfected cells were infected by concentrated SARS-CoV-2 pseudotyped virus 48 h later ...
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bioRxiv - Biophysics 2021Quote: ... and transfected at 80–90% of confluency using X-tremeGENE™ HP DNA Transfection Reagent (Roche) following strictly the manufacturer protocol.
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bioRxiv - Cell Biology 2020Quote: ... Gag/Pol and VSV-G using Fugene-6 transfection reagent as per the manufacturer’s instructions (Roche) for 8 hours ...
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bioRxiv - Microbiology 2020Quote: ... and were transfected with indicated plasmids by the X-tremeGENE HP DNA Transfection Reagent (Roche, USA) when the cell confluence was up to 90% ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected 48 hours post-transfection and sonicated in PBS with protease inhibitor mixture (Roche). Samples were processed and analyzed by western blot as described above.
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bioRxiv - Molecular Biology 2022Quote: ... structure in the 5’UTR and 70 ng of firefly luciferase (FLuc)-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... or a HP50 hairpin structure in the 5’UTR and 2 ng of FLuc-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... UL16 and UL21 expression plasmids using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2.5 ug plasmid DNA per well using DOTAP Liposomal Transfection Reagent (Roche) following manufacturer’s instructions ...