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Citations for Roche :
251 - 300 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: RNAs were extracted 48 hours after RNP transfection using the High Pure RNA Isolation Kit (Roche) according to instructions of the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... D-Mel (d.mel-2) cells were transfected using X-tremeGene HP DNA transfection reagent (#06366236001, Roche). Two days after transfection ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were transfected into HeLa cells using X-tremeGENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and pLentiCRISPRv2-PFN1sgRNA (500 ng each) into HEK293FT cells using X-tremeGENE HP transfection reagent (Roche). Lentivirus was harvested 72 hours later and subsequently used to infect JR20 cells supplemented with 4 μg/mL of Polybrene ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with Lpd-specific or scramble shRNA as control using Fugene HD (Roche) transfection reagent ...
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bioRxiv - Molecular Biology 2019Quote: ... and CAT expression was quantified using the CAT ELISA (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and pLenti-Luc-GFP (6 µg) into HEK293T cells using X tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer′s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 15 minutes with 4 μL of X-treme Gene HP DNA transfection reagent (Roche). Transfected cells were selected for by hygromycin resistance ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa WT cells were transfected with the guide containing plasmid using the transfection reagent XtremeGENE HP (Roche) according to manufacturer’s protocol ...
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The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cellsbioRxiv - Molecular Biology 2021Quote: ... The pDCs were left untreated or stimulated with 1μM CpG 2216 complexed to transfection reagent DOTAP (Roche) for 2h ...
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bioRxiv - Molecular Biology 2021Quote: ... BMDMs were transiently-transfected with tDR-GlyGCC-30-AF647 (1μg/mL) complexed to DOTAP transfection reagent (Roche) for 24h in serum-free media followed by nHDL (1mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T at 60-80% confluence were transfected with plasmids amplified from an overexpressing lentiviral library together with 0.975µg CMVΔR8.74 and 0.525µg VGV.G packaging plasmids using X-tremeGENE™ HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell lines were transfected with siRNAs using xTremeGene siRNA transfection reagent (1ul/well, Roche, #4476093001; standard protocol). After addition of siRNA transfection mix ...
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bioRxiv - Microbiology 2020Quote: ... One step real time RT-PCR was performed using the RNA Process Control Kit (Roche, Basel, Switzerland) with 5 μl of extracted RNA or directly from 5 μl of heated sample ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Molecular Biology 2020Quote: ... All libraries and controls went through 13 PCR cycles using KAPA HiFi HotStart Ready Mix (KAPA Biosystems). PCR products were size-selected on a 1.8% agarose gel before loading on a Novaseq (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), mouse anti-Pgk1 (459250 ...
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bioRxiv - Developmental Biology 2022Quote: ... opilio used sense (control) and antisense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland) and followed published protocols (Sharma et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The QUB GTCU generated libraries (3x control and 3x irradiated) using the KAPA mRNA HyperPrep Kit (Roche) prior to paired-end sequencing (2 x 50 bp ...
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bioRxiv - Cell Biology 2022Quote: ... containing protease and phosphatase inhibitors (Roche, cat# 11697498001 and cat# 4906845001) and 1x benzonase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then transfected with the respective plasmids using X- tremeGENE™ HP DNA transfection reagent (Roche, #6365779001) for 48 h (24 h for Toca-1 and Cdc42).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of heavy- and light chain pDNA were mixed with X-tremeGENE HP DNA Transfection Reagent (Roche) in FreeStyle™ 293 Expression Medium (Thermofisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or p89.6) and an envelope deficient HIV-1 backbone vector (pSG3Δenv) using XtremeGENE HP DNA Transfection Reagent (Roche). After 24 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: The plasmids FUG-T2A-GFP-Cre and control GFP were purchased from Addgene #66691 and lentiviral particles were produced in HEK-293T cells using X-tremeGene 9 DNA Transfection Reagent (Roche). Upon ultracentrifugation concentrated virus particles were stored at −80°C and used to infect pancreatic ductal organoid cultures as previously described (Huch et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... FMO05476) and Renilla reporter (pRL-TK Vector) by using the X-treme GENE HP DNA Transfection Reagent (Roche). After 48 h of transfections ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 20 mM siRNAs and 3 µL of X-tremeGENE HP DNA Transfection Reagent (Roche, #6366546001) were diluted in 200 µL Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... The three SCC cell lines were transiently transfected with the expression plasmids using FuGENE HD transfection reagent (Roche Molecular Systems ...
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bioRxiv - Developmental Biology 2019Quote: ... Transfection targeting the AAVS1 locus was performed with X-TremeGene 9 following the manufacturer’s instructions (Roche, Indianapolis, IN).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HCC827 and H661 cells were transfected with the siRNAs using X-tremeGENETM siRNA Transfection Reagent (Roche, Mannheim, Germany) according to the manufacturer’s manual when the cells reached a confluence of 60-80% ...
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Genetics 2020Quote: ... The mini-genes in the pSPL3b vector were transiently transfected using 6µl of FuGENE 6 Transfection Reagent (Roche) with 2 µg of vector ...
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bioRxiv - Developmental Biology 2022Quote: ... The next day cells were transfected using X-tremeGENE HP DNA transfection reagent (Roche 06-366-236-001) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... miR-122 inhibition was carried out by transfection with AntagomiR 122 (Miravirsen, SPC-3649; Roche, formerly Santaris Pharma). For Smad4 silencing in mouse primary hepatocytes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 1 µg/mL plasmid DNA using Fugene HD transfection reagent (Roche Diagnostics, Indianapolis, IN).
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Neuroscience 2023Quote: ... Vectors were co-transfected with packaging-defective helper plasmids into 293T cells using Fugene 6 transfection reagent (Roche). Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich ...
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bioRxiv - Pathology 2023Quote: ... 100 ng pCMV4-BlaM-Vpr and 60 ng pAdVAntage ™ Vector using X-treme gene transfection reagent (Roche). Supernatant was harvested at 48 and 72 h post-transfection ...
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bioRxiv - Genetics 2023Quote: ... 48 hrs after transfection, the cells were washed with DPBS and lysed (1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run in triplicates along with no template control in a LightCycler® 480 Instrument II (Roche). The rpoB gene encoding for DNA-directed RNA polymerase subunit beta was used as housekeeping control [73] ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was incubated overnight (O/N) at 4°C with mouse anti-α-tubulin (Roche, loading control) at 1:4000 ...
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bioRxiv - Immunology 2021Quote: ... An equimolar amount of DMSO was added as a negative control and phytohemagglutinin (PHA, Roche (San Diego, CA) 1 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... An equimolar amount of DMSO was added as a negative control and phytohemagglutinin (PHA, Roche (San Diego, CA) 1 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Cancer Biology 2022Quote: ... as well as 165 pg Bisulfite-Conversion Control (viral unmethylated gDNA; SeqCap Epi Accessory Kit; Hoffmann-La Roche) were fragmented to an average size of 200 20 bp using the Covaris M220 (Covaris ...
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bioRxiv - Pathology 2020Quote: ... The inter-assay coefficient of variation was <1.65% using a frozen serum pool and <1.66% using quality control samples from Roche.
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bioRxiv - Cell Biology 2019Quote: Control BMSCs were lysed by incubation with RIPA buffer (Pierce) and 1 mM protease inhibitors (Complete Mini, Roche). Samples were incubated with 5X Lane Marker Reducing sample buffer (Pierce ...
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bioRxiv - Pathology 2020Quote: ... The inter-assay coefficient of variation was < 5.06% using a frozen serum pool and < 2.36% using quality control samples from Roche. Glucose (mmol/L ...
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bioRxiv - Cell Biology 2023Quote: ... Total extracts from control and patient fibroblasts were prepared in RIPA buffer supplemented with protease inhibitors (Complete, Roche). Five micrograms of isolated mitochondria or twenty micrograms of total protein extracts were resuspended in Laemmli buffer ...