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Citations for Roche :
3201 - 3250 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... PCR enrichment of fragments containing transposon sequence was then carried out using transposon- and adaptor-specific primers using Kapa HiFi HotStart ReadyMix (Kapa Biosystems) for 10 to 20 cycles ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 3 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed by resuspension in 10mL cold NP-40 lysis buffer (150mM NaCl, 10mM Tris pH 7.2, 1% NP-40, 5mM DTT, 2mM MgCl2) containing protease inhibitors (Roche, Laval, QC) on ice for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: Protein was extracted from iNs by lysis in NP-40 lysis buffer (1% NP40, 0.5M EDTA, 5M NaCl, 1M Tris) containing cOmplete protease inhibitors and phosSTOP (Roche, Penzberg, Germany). Lysates were analyzed using the Multi-Spot Phospho (Thr 231)/Total Tau ELISA (Meso Scale Discovery ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were washed and lysed in 2 mL of lysis buffer (containing in mM: 50 Tris pH 7.2, 5 EDTA, 150 NaCl, 10 NEM, Protease Inhibitor Cocktail (Roche, Basel, Switzerland), 2 PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative PCR was performed with 2 µL of cDNA as a template in a total volume of 20 µL containing LightCycler FastStart DNA MasterPlus SYBR Green I (Roche, USA) and 0.5 µM primer ...
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bioRxiv - Neuroscience 2023Quote: 30 heads of 8-10 day-old-males per genotype were homogenized in 30 μL Laemmli buffer containing protease inhibitor (Complete mini Protease Inhibitor Cocktail, Roche Diagnostics), using a Minilys apparatus (Bertin Instruments ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with cold phosphate-buffered saline (PBS) and lysed with NP-40 lysis buffer (P0013F, Beyotime, China) containing protease inhibitor cocktail (04693132001, Roche, Switzerland). Cell lysates were incubated overnight at 4 [with Dynabeads (Sc-2003 ...
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bioRxiv - Physiology 2024Quote: ... one lobe of dissected adipose tissue was homogenized with a Teflon pestle in RIPA buffer containing protease (cOmpleteTM Mini, Roche, Germany) and phosphatase (5 mM Na fluoride ...
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bioRxiv - Cell Biology 2024Quote: The whole lysates of AT or AT-EVs was prepared on ice by using the RIPA lysis buffer (Beyotime, China) containing protease and phosphatase inhibitors (Roche, Germany). After lysis for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... cDNAs were diluted to 1 ng/ µL and used for qPCR analysis in a 10 µL reaction mix containing 5 µL of LightCycler® 480 SYBR Green I Master mix (Roche), 300 nM of each primer ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μl papain inhibitor solution containing 5 mg/ml BSA (Carl Roth, 8076.4) and 5 mg/ml Trypsin inhibitor (Sigma-Aldrich/Roche, 10109878001) in PBS was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were collected by centrifugation at 3000 x g and resuspended in 1X PBS lysis buffer containing 1mM PMSF and protease inhibitor cocktail (Roche, 5056489001). WCEs were prepared by vortexing with glass beads ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Microbiology 2023Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain with known weight was homogenized at 4°C using automated homogenizer and with IX RIPA buffer containing protease inhibitor tablet (Roche, Germany). After that the brain lysates were centrifuged at 12,000 rpm for 10 mins ...
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bioRxiv - Biochemistry 2023Quote: ... qPCRs reactions were prepared in a 20 uL final volume containing Fast Start Universal SYBR Green Master (Rox) (Roche Applied Science), cDNA template ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in a 0.2 ml PCR tube preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM P5 transposon primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Neuroscience 2023Quote: ... was performed in HB buffer (20 mM Na phosphate pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH) and nuclei were eliminated by 900 x g centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... The resulting cell pellets were resuspended in Ni column buffer (25 mM Tris HCl, 50 mM NaCl, 30 mM imidazole pH 7.4) containing protease inhibitors (Roche Applied Science). Cells were lysed with 4 passes through an Emulsiflex C3 (Avestin) ...
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bioRxiv - Immunology 2023Quote: ... then subject to gentle cold digestion on ice using 10% FBS-supplemented RPMI media containing 100mg/mL trypsin inhibitor from soybean (Roche, 10109886001), 10mg/mL collagenase A from clostridium histolyticum (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... the aerial parts were dipped for 30 s in 1 ml of 0.01% (v/v) Tween-20 aqueous solution containing 50 µM MG132 (Roche Sigma-Aldrich) in DMSO ...
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bioRxiv - Pathology 2023Quote: ... The tissues were cut into small pieces with scissors and incubated in 3mL of RPMI containing 900µg of Liberase TM (Roche, Basal, Switzerland) and 150µg DNase I (MP Biomedicals ...
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bioRxiv - Physiology 2023Quote: ... Then the heart was perfused with the same solution containing 1 mg/mL collagenase Type A (Catalog # 9036-06-0; Roche, Germany) and 0.08 mg/mL protease Type XIV (Cat log # 48655321 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 2X with cold DPBS++ and RIPA buffer (Pierce #89900) containing complete EDTA-free protease inhibitor cocktail (Roche #11873580001) was added to wells ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were homogenized on ice in lysis RIPA buffer (Thermo Fischer Scientific, Waltham, USA) containing protease inhibitor cocktail (Roche Diagnostics, Switzerland). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...
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bioRxiv - Biochemistry 2023Quote: 106 cells were washed with 3 mL of cold PBS and resuspended in 50 µL solution containing 100 µg/mL neuraminidase from Clostridium perfringens (Roche, #11585886001). Cells were incubated for 1 h at 37 °C and washed twice with PBS before incubation with LiLA.
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bioRxiv - Evolutionary Biology 2023Quote: ... Each eluate (3 µl) was subjected to PCR amplification in 50-μl reactions containing 1 KAPA HiFi HotStart Uracil+ReadyMix (Kapa Biosystems) and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Immunology 2023Quote: ... Uteri were isolated and carefully dissected to remove the yolk sac containing the fetus followed by mechanical disruption and digestion with DNase I 200 units/mL (Roche 10104159001) and Liberase DL 0.3 W units/mL (Roche 05466202001 ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Biophysics 2024Quote: ... murine eyes were freshly dissected using cold phosphate-buffered saline without Ca2+ and Mg2+ (PBS-/-) and containing protease inhibitors as instructed by the manufacturer (Roche, 4693132001). Dissected eyes were denuded by the RPE layer via incubation of the tissue for 60 minutes with 50 mM Tris–HCl buffer plus 20 mM ethylenediamine tetraacetic acid (EDTA) ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing either 40mg/L hygromycin (Roche, no. 10843555001) or 25mg/L DL-phosphinothricin (Duchefa ...
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bioRxiv - Developmental Biology 2024Quote: ... The discs were dissected in pre-cooled PBS and pipetted in PBT (PBS, 0.1 % Triton X-100) containing 1x proteases inhibitors (cOmplete™ Protease Inhibitor Cocktail tablets, Roche, 11873580001). The disks were incubated in PBT with 1x protease inhibitors and 0.4 % IGEPAL CO-630 (Sigma ...
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bioRxiv - Genetics 2024Quote: ... cells were resuspended in 3 ml lysis buffer (PBS containing 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and cOmplete proteinase inhibitor [Roche Diagnostics]), lysed by ultrasonic treatment and incubated with 1.2 ml NeutrAvidin Agarose for 0.5 h at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... plasmids to be barcoded were diluted to 2 ng/µl and PCR amplified using the primers containing the full barcode sequence using KAPA HiFi HotStart ReadyMix (KK2602, Roche, USA). Barcoded PCR products were run on a 1.2% agarose gel ...
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bioRxiv - Cancer Biology 2021Quote: ... 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cancer Biology 2021Quote: ... The exome captured by the SeqCap wash kit and SeqCap pure capture bead kit (both from Roche). The Illumina Novaseq platform was used to sequence 150 bases (paired-end sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Plant Biology 2022Quote: ... The EMSA was carried out utilizing the manufacturer’s instructions (DIG-gel shift kit 2nd generation kit, Roche).
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bioRxiv - Genomics 2019Quote: ... using the DNA 1000 kit and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of Hiseq2500 in single read 100nt mode using the clustering and SBS v3 kit following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were subjected to total RNA extraction using a commercially available kit (High Pure kit, Roche, Indianapolis, IN). Total RNA was then used to make complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...
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bioRxiv - Epidemiology 2019Quote: ... An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems, Wilmington, MA). The amplicon pool was sequenced on the Illumina MiSeq generating paired end 300 bp reads ...