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Citations for Roche :
3451 - 3500 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The KAPA HyperPrep Kit (Roche, Basel, Switzerland) was used in a 15 μL reaction for end repair and A-tailing ...
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bioRxiv - Cell Biology 2022Quote: ... and the Chromo-Map DAB kit (Roche). The following primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... and the KAPA library quantification kit (Roche) with triplicate measurements ...
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bioRxiv - Developmental Biology 2023Quote: The High Pure RNA Isolation Kit (Roche) was used for RNA extraction from cultured cells ...
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bioRxiv - Cancer Biology 2023Quote: ... using SYBR Green 1 Master Kit (Roche). PCR amplification was performed as described (26) ...
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bioRxiv - Developmental Biology 2023Quote: ... and KAPA Library Quantification Kits (KAPA Biosystems). The size of sequencing libraries was determined by means of Agilent D5000 ScreenTape system at Tapestation 4200 system (Agilent) ...
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bioRxiv - Bioengineering 2023Quote: LDH Cytotoxicity Detection Kit plus (Roche Diagnostics) was used to quantify U2OS cell death on day 1 and day 7 of cell culture ...
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bioRxiv - Genomics 2023Quote: ... with KAPA2G Robust kits (KAPA Biosystems, KR0379). An additional set of primers against spike-in lambda phage DNA is performed concomitantly to evaluate the effects of A3A deamination (bACE ...
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bioRxiv - Cancer Biology 2023Quote: ... using the KAPA qPCR quantification kit (Roche). The libraries were normalized and pooled as per manufacturer protocol (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a Hyper library construction kit (Kapa biosystems) was used to prepare paired end libraries of around 400-500 bp inserts ...
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bioRxiv - Cancer Biology 2024Quote: ... using High Pure RNA Isolation Kit (Roche), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and the KAPA Library Quantification Kit (Roche) before proceeding with sequencing using NovaSeq 6000 (Illumina).
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bioRxiv - Bioengineering 2024Quote: ... High Pure RNA Isolation Kit (11828665001, Roche); LIVE/DEAD viability/cytotoxicity Kit (L3224 ...
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bioRxiv - Neuroscience 2024Quote: ... and KAPA Library Quantification Kit (Roche, KK4854). Finally ...
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bioRxiv - Cancer Biology 2024Quote: TUNEL apoptosis assay kit (Roche Diagnostics GmbH) was used to assess tumor apoptosis following fixation in 4% paraformaldehyde with vacuum overnight at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The KAPA HyperPrep kit (Roche, Basel, Switzerland) was used for library preparation ...
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bioRxiv - Genetics 2024Quote: ... KAPA HiFi HotStart PCR Kit (Roche; KK2502) was used for indexing-amplification with NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and Roche SeqCap Adapter kit (Roche, KR1317) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... A PCR DIG probe synthesis kit (Roche) was used for visualization of the dsDNA probe ...
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bioRxiv - Molecular Biology 2024Quote: ... or SeqCap EZ MedExome Enrichment Kit (Roche) and sequenced on a HiSeq 4000 or HiSeq 2500 platform (Illumina) ...
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bioRxiv - Plant Biology 2024Quote: ... A Faststart Taq Polymerase Kit (Roche, UK) was used for PCR following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... one with the Illumina Genomic DNA Sample Prep kit and one with the Kapa Hyper kit (Kapa Biosystem, Roche). Three mate-pair libraries were constructed using Illumina Nextera Mate Pair Library Prep kit with insert sizes ranging between 2-4 kb ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were validated for quality on Agilent DNA1000 Kit and quantified with the qPCR NGS Library Quantification kit (Roche). The final libraries were equimolar pooled and sequenced using the High Output 1×75 kit on the Illumina NextSeq500 platform producing 75bp single-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by in vitro transcription with DIG-RNA labelling Kit or Fluorescein-RNA labelling kit (Roche, Switzerland). The integrity of probes was determined by gel electrophoresis and storage in 50% RNAlater (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Capture library concentrations were determined by qPCR with a KAPA qPCR kit (KAPA Library Quant Kit, Kapa Biosystems, USA) and pooled at the same concentration for sequencing ...
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bioRxiv - Genetics 2021Quote: ... Capture-C libraries were made using the Arima HiC kit (Arima Genomics) and the KAPA HyperPrep kit (KAPA Biosystems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The absence of kit/reagent contamination was verified in the High Pure Viral Nucleic Acid Kit (Roche Applied Science) and Illustra™ GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... and the second-strand cDNA was synthesized using a KAPA Biosystems kit (Roche KAPA HiFi Hotstart PCR kit, KK2502) with VHgene-specific primers (No ...
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bioRxiv - Biochemistry 2024Quote: ... An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems, Wilmington, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Microbiology 2020Quote: ... The cross-linking reaction was quenched by incubating the cells with 0.125 M glycine for 5 min with mild agitation at room temperature followed by centrifugation at 3,000 rpm for 5 min at 4°C with a subsequent PBS wash (containing 0.01X protease inhibitor cocktail or PIC; #11836170001, Roche Applied Science, Indianapolis, USA). Following the complete removal of PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) containing phosphatase inhibitors (10 mM sodium pyrophosphate, 20 mM β-glycerophosphate, 100 mM NaF) and protease inhibitor cocktail (Roche Applied Science). Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz ...
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Proto-sex locus in large yellow croaker provides insights into early evolution of the sex chromosomebioRxiv - Evolutionary Biology 2020Quote: ... The DNA probes constructed from a BAC clone containing dmrt1 (supplementary note 11) were labeled with biotin-11-dUTP using a Nick Translation System (Roche Diagnostics, Basel, Switzerland). The probes were added to the hybridization buffer ...
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bioRxiv - Developmental Biology 2020Quote: Approximately 100 mg of minced PND12 rat ovary tissue was digested in 1ml of digestion medium [199 media containing 0.08 mg/ml of liberase with medium-concentration of thermolysin (Roche Diagnostics GmbH, Mannheim, Germany) supplemented with 5U/ml of DNase I and 1% BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The extracted viral DNA and a serial dilution of the transfer plasmid DNA containing the WPRE sequence were transferred to a 96 well plate and measured using a LightCycler® 96 instrument (Roche Life Science). AAVs produced had a titer of 2.10 × 1013 viral genomes/ml.
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation (16 h at 4 °C) in the presence of 10 pM TBST containing complete TM protease inhibitor cocktail (Roche Pharma, Basel, Switzerland). The membranes were washed three times with TBS (150 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... This was repeated twice followed by incubation for 1 hour at 37°C in a solution containing Collagenase D (Roche; 1.5 U/ml), Dispase II (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... One μl of each eluted supernatant-DNA sample was added to a reaction mixture containing 2X Light Cycler480 SYBR Green I master mix (Roche Applied Science, Germany) and 0.25 μM of forward and reverse primers (ALU-115 and ALU-247 ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Systems Biology 2021Quote: Frozen cell pellets were resuspended in 1x IPOD lysis buffer (10 mM Tris HCl, pH 8.0; 50 mM NaCl) containing 1x protease inhibitors (Roche Complete Mini, EDTA free) and 52.5 kU/mL of ready-lyse (Epicentre) ...
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bioRxiv - Microbiology 2019Quote: ... The obtained cDNAs were diluted 10 times in water and quantitative PCR was performed using 2.5 μL of each diluted cDNA in a total volume of 10 μL containing 1× LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics, cat. #04707516001) and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Freshly frozen hearts were homogenized in RIPA buffer containing phosphatase inhibitor cocktail I (Wako) and protease inhibitor tablet mini (Roche, cOmplete™, Mini), using a hand held homogenizer on ice for 1 min ...
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bioRxiv - Microbiology 2021Quote: 48 h bacterial cultures were pelleted and were resuspended in RIPA buffer containing protease inhibitors cocktail (Roche, Cat. No. 11 836 145 001). Samples were sonicated at 50% voltage for 5 cycles of 10 sec pulses followed by 30 sec rest on ice ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed with ice cold PBS and lysed in RIPA buffer containing phosphatase and protease inhibitors (Roche, South San Francisco, CA). For mouse and human biopsies ...
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bioRxiv - Neuroscience 2021Quote: Samples from the 2D cultures or brain organoids were collected in RIPA buffer containing protease inhibitors (Complete mini, Roche Applied Science, Penzberg, Germany) and sonicated two times at 60% - 70% during 10 s before use for the western blotting analyses ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Neuroscience 2022Quote: ... They were scooped with a brush and dropped in a glass tube containing the homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) at 4°C (50 μl of buffer for each slice) ...
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bioRxiv - Genomics 2019Quote: Donor organs were obtained with written consent and research ethics approval at the University of Alberta (Pro00013094, Pro00001754), perfused via the pancreatic ductal system with buffer containing Collagenase Gold 800 (VitaCyte, Indianapolis, IN) and Thermolysin (Roche Diagnostics, Mannheim, Germany), then digested with a Ricordi Islet Isolator (Biorep Diabetes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... tissues or cells were homogenized in a RIPA buffer containing cOmplete Protease Inhibitor Cocktail and PhosphoSTOP Phosphate Inhibitor Cocktail Tablets (Roche, Indianapolis, IN, USA) using a Bead Ruptor 24 Elite bead mill homogenizer (Omni International ...