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Citations for Roche :
2751 - 2800 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin was then de-crosslinked overnight at 65°C in the presence of proteinase K (Roche), purified by phenol and phenol-chloroform extractions ...
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Biochemistry 2021Quote: ... incubated at 37°C for 30 min with 0.5 mg/ml liberase DL (Roche Basel, Switzerland) and 100 U/ml DNase (Roche Basel ...
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bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at 37 °C and then with ∼1.2 mg/mL Proteinase K (3115879001, Roche) overnight at 65°C ...
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bioRxiv - Immunology 2019Quote: ... Tissue was transferred to C-tubes (Miltenyi) and digested using Liberase™ (Roche, 57.6 μg/ml) in the presence of DNase I (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Annealed oligonucleotides were subsequently phosphorylated for 30 minutes at 37°C using T4 polynucleotide kinase (Roche). 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche) ...
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bioRxiv - Immunology 2020Quote: ... -C and -G in a pX330 vector was transfected into HAP1 cells using X-tremeGENE (Roche). Single cells were FACS sorted based on W6/32 negativity to obtain knockout clones for HLA-A ...
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bioRxiv - Microbiology 2019Quote: ... Contaminating DNA was digested by DNase I (Roche; 1 U/µg RNA, 60 min, 37°C) in the presence of RNase inhibitor (RNaseOUT ...
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bioRxiv - Microbiology 2019Quote: ... Microarrays were hybridized for 20 hours at 42°C with the NimbleGen Hybridization System (Roche NimbleGen). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... Viruses were treated for 30 min at 37°C with 1,000 U of DNase I (Roche). As a control ...
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bioRxiv - Cancer Biology 2022Quote: ... and stored at least 1 hour at −20 °C before staining with Tdt reaction mix (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... at 42 °C and detection was performed by chemiluminescence using anti-Digoxigenin-AP Fab fragments (Roche) and CDP-Star chemiluminescent substrate (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... embryos were washed with hybridization buffer at 55°C and incubated with Western Blocking Reagent (Roche) at room temperature for ∼2 hours ...
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bioRxiv - Physiology 2024Quote: ... digested for 30 minutes at 37°C with 0.5-1 µg/ml of proteinase K (Roche), quickly rinsed with cold PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were washed three times in Buffer C complete including 1x complete protease inhibitors (Roche) and 0.5 mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Genomics 2023Quote: ... final extension 5 min at 62 °C) using the KAPA HiFi HotStart Ready Mix (Kapa Biosystems). Amplified libraries were purified ...
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bioRxiv - Biochemistry 2023Quote: HDX of unbound and bound c-Met (1:2.2 ratio with mAb (Roche Diagnostics GmbH, Germany)) was performed by diluting c-Met into labelling buffer (20mM Histidine ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Neuroscience 2023Quote: ... DRG were incubated for 1 hour at 37 °C with 1 mg/ml dispase II (Roche) and 2 mg/ml collagenase A (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 720 μL concentrated solution was treated with 1000U/mL DNase I (37°C, 2h) (Roche, China) before viral DNA extraction ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 1 hour at 37⁰C and 5% CO2 with 10U DNase (Roche) in R10 medium (RPMI 1640 medium containing GlutaMAX from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 0.3% Tween-20 pH 7.5) for 5 min at 37°C and blocking solution (#11585762001, Roche) for 45 min at 37°C ...
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bioRxiv - Immunology 2024Quote: PCLS (4mm diameter) were incubated (37°C, 5% CO2) with 10µL of WST-1 reagent (Roche) in 100µL of culture media for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in lysis buffer (50 mM Tris pH 7.4, 200 mM NaCl) supplemented with protease inhibitor tablets (complete EDTA-free; Roche, one tablet per 250 mL of lysis buffer), lysozyme (0.1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... per sample was washed 3 times with cold 1X PBS and 2μg anti-GFP monoclonal antibody (Roche) per sample was conjugated with Dynabeads in 1ml cold PBS at 4°C for 4h ...
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bioRxiv - Microbiology 2019Quote: ... and CO2 reverse primer (5′-TACCTTGTTACGACT-3′)53 with KAPA Lightcycler 480 mix (KAPA Biosystems Ltd., UK) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 25µl of Red ANTI-FLAG M2 Affinity Gel from SIGMA ALDRICH (F2426) were washed 3 times with TBS (50mM TRIS pH 7.5,150mM NaCl and protease inhibitor tablets [Roche]) and then added to samples ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl Universal Nuclease Mix (Pierce™) and 1 tablet of cOmplete™ Protease Inhibitor Cocktail (Roche) were added and the cells were lysed via sonication ...
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bioRxiv - Genetics 2020Quote: Exon 3 of the MSH2 gene was PCR amplified using the Expand High Fidelity PCR kit (Roche) with the following conditions ...
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bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).
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bioRxiv - Immunology 2020Quote: ... gently mechanically disaggregated and resuspended in PBS 1x containing 3 mg/ml of collagenase D (Roche Diagnostics) plus 10 μg/ml of DNAse (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The harvested molars were pooled and dissociated with 3 U/mL Collagenase P (COLLA◻RO, ROCHE) followed by incubation for 45◻minutes in a 37°C shaking water bath ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
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bioRxiv - Neuroscience 2022Quote: ... R: 5’-GACCTGCAGGAGGATCGTAG −3’) was determined by qPCR using FastStart Essential DNA Green Master Mix (Roche, 06402712001) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Chloroplast were lysed osmotically in buffer 3 (25mM HEPES-KOH, pH 8.0) containing cOmplete protease inhibitor (Roche) and either separated into soluble and pellet fraction by centrifugation for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell extracts were obtained by washing 3 × in PBS and either lysing for RNA (TriPure reagent, Roche) or protein extraction (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... 3 mM MgCl2; 0.1 % NP-40; 0.2 U/µl RNaseOUT; 0.32 M Sucrose; 1x protease inhibitor, Roche) and transferred to 2 ml Dounce tissue grinder (Sigma) ...
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bioRxiv - Genomics 2023Quote: Mastermix 3 (IS-PCR) was freshly prepared and contained 12.5 μl Kapa HiFi Hotstart Readymix (2x, Roche), 0.25 μl IS PCR Primers (10 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... R-5’-GGGACGCAGCAACTGACATT-3’) was assessed by RT-qPCR using SyBR Green solution on a LightCycler480 (Roche). The cDNAs of every single cell were purified using the DNA Clean & Concentrator Kit (Zymo ...
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bioRxiv - Systems Biology 2023Quote: ... 3 mM MgCl2 and 0.1% IGEPAL CA-630) supplemented with 0.2U/µl of RNAse Protector (Roche, Switzerland), was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... 3 ng ds-cDNA was processed for library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol except that a 15-min USER enzyme (BioLab # M5505L ...
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bioRxiv - Bioengineering 2022Quote: A total of 3 µL of AAV was treated with 20 units of DNase I (Roche #04716728001) at 37°C for 45 min to remove residual DNA in vector samples ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were homogenized 3 x 30s at 7000 power in a MagnaLyzer® (Roche Diagnostics, Basel, Schweiz) and placed on ice for ∼1 minute between each homogenization ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.5 mM spermidine, 0.2 mM spermine, 0.01% Triton-X, 1x Roche complete protease inhibitors ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 3 hours with Anti-Digoxigenin-AP Fab fragments (dilution 1:3000) (Roche, 11093274910) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 0.25Msucrose, 3 mM MgCI2, 0.2% NP-40, 3mM p-mercaptoethanol, 0.4mMPMSF, Complete protease inhibitor tablets from Roche). Chromatin fragmentation was performed by MNase treatment in ChlP SDS lysis buffer (50mMHEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... They are incubated for at least 4 hours in blocking solution 1x WBR (Roche, 11921673001) and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: For sequencing all samples were treated with 4 mg/ml of proteinase K (Roche Diagnostics). RNA and small RNA were extracted and enriched in separate fraction from the supernatants using the mirVana kit according to manufacturer’s instructions to separate samples into two groups ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μL of the supernatant were incubated with 20 μg/mL proteinase K (PK; Roche) in a total volume of 22 μL RIPA buffer for 1 hr at 37°C to digest all proteins except for PK-resistant PrPSc ...