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Citations for Roche :
2551 - 2600 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 with with 2X cOmplete Protease Inhibitor Cocktail EDTA-free (Roche)) for 8 minutes ...
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bioRxiv - Biophysics 2019Quote: ... Following re-suspension in 3 ml of medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions of lung cells were prepared by Liberase Blendzyme 3 (Roche) digestion of perfused lungs as previously described48 ...
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bioRxiv - Microbiology 2021Quote: ... based on the 5′ and 3′ RACE kit (2nd generation, Roche, Basel, Switzerland). Viral nucleic acids were extracted from 140 μl of the virus transport medium derived from the nasopharyngeal swab using the viral RNA isolation kit according to the manufacturer’s protocol (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by digesting the RNA with 3 µg RNase A (Roche) and 30U RNase T1 (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.2% bromophenol blue) and treated with 3 mg/ml Proteinase K (Roche) for 1 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche) at 6,000 rpm for 15 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche) and dGTPs ...
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 3 minutes 10 μL of 5 mg/mL Liberase (Roche, cat # 05401119001) were added ...
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bioRxiv - Developmental Biology 2023Quote: ... After a brief enzymatic treatment with a cocktail of Liberase Blendzyme 3 (Roche), trypsin B (BI) ...
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bioRxiv - Developmental Biology 2021Quote: ... Signals were developed using 4-nitro blue tetrazolium chloride (NBT, Roche) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Cell Biology 2022Quote: ... 4 mM MgSO4) containing Complete Inhibitor Protease Cocktail (Roche, Indianapolis, IN), lysozyme and DNAse ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 complete-EDTA protease-inhibitor tablets per 500 mL (Roche). After thawing ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA (4 ng) and LightCycler 480 SYBR Green I Master (Roche) in a final volume of 10 μl ...
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bioRxiv - Neuroscience 2024Quote: ... and detection with 4-Nitro blue tetrazolium chloride (NBT; 11383213001, Roche) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Neuroscience 2023Quote: ... 4% BlockAce (DS Pharma Biomedical) and 0.5× Blocking reagent (Roche Diagnostics)] for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemistry was performed on 4 μm sections using the BenchmarkUltra (Roche), anti-hCD45 (M0701 ...
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bioRxiv - Plant Biology 2023Quote: ... 1/200 mM NaF) containing 4 tablets of protease inhibitor (Roche cOmplete ...
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bioRxiv - Genetics 2020Quote: ... The DIG probe was hybridized to the membrane (at 44°C; using Roche DIG Easy Hyb Granules for hybridization buffer ...
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bioRxiv - Biochemistry 2020Quote: ... or endoproteinase Glu-C from Staphylococcus aureus V8 (Roche Diagnostics GmbH, Mannheim, Germany). Enzyme to protein ratios were 1:20 ...
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bioRxiv - Developmental Biology 2022Quote: ... then hybridized overnight at 65 °C with digoxigenin-labeled RNA probes (Roche, 11277073910). The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Genomics 2019Quote: ... 62°C for 5 min) using KAPA HiFi HotStart ready mix (KAPA Biosystems) and NextFlex primer mix (Bio Scientific ...
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bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
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bioRxiv - Plant Biology 2021Quote: ... and immunolabeled with specific antibodies against anti-c-Myc (1:1000, E910, Roche), anti-GFP (1:10000 ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated for 1 hour at 37°C with 10U DNase (Roche) in R10 medium (RPMI 1640 medium containing GlutaMAX from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Colonic tissues were digested at 37°C for overnight with Collagenase A (Roche) and DNase I (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.4 at 23°C) supplemented with EDTA-free protease inhibitor cocktail (Roche) at approximately 7 ml per gram of wet weight and subjected to 3 rounds of disruption on a high-pressure homogenizer (Avestin ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins were digested for 18 h at 37°C with chymotrypsin (Roche, Germany), followed by 3 h at 37°C using trypsin (Promega) ...
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bioRxiv - Genomics 2020Quote: ... Micro-C library was generated by using KAPA HiFi HotStart ReadyMix (Roche #KK2601) or Q5 High-Fidelity 2X Master Mix (NEB #E7645 ...
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bioRxiv - Molecular Biology 2021Quote: ... digested rotating for 1h at 37°C with 2mg/ml Collagenase/Dispase (Roche) and then filtered twice (100 μm ...
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bioRxiv - Genomics 2021Quote: ... The Hi-C library was quantified using a KAPA library quantification kit (Roche), and further PCR amplification was performed using Phusion Hot Start II DNA polymerase (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... at 37°C for 30 min and 0.1 mg/mL proteinase K (Roche) at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Molecular Biology 2023Quote: ... Urine was analyzed for Protein and Albumin (Cobas c 311 Analyzer; Roche Diagnostics).
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bioRxiv - Systems Biology 2024Quote: ... then 5 min at 62°C) with KAPA HiFi HotStart Ready Mix (Roche). Libraries were quantified by Bioanalyzer (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... then hybridized overnight at 65 °C with digoxigenin-labeled RNA probes (Roche, 11277073910). The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 37°C for 30 minutes in proteinase K solution (Roche Diagnostics ...
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bioRxiv - Genetics 2024Quote: Ide genotype rs30920120 (C/G) was assayed using LightCycler 480 Instrument II (Roche) or the TaqMan method (Applied Biosystems Inc ...
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bioRxiv - Immunology 2024Quote: ... then were digested for 20 min at 37 °C with Liberase TM (Roche) plus DNase I (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was refreshed one time before the addition of Liberase TM research grade (final concentration 0.038 mg/mL (Roche Diagnostics, GmbH, Mannheim, Germany)) and Elastase from porcine pancreas (final concentration 0.01 mg/mL (Bio-Connect B.V. ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... They were scooped with a brush and dropped in a glass tube containing the homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) at 4°C (50 μl of buffer for each slice) ...
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bioRxiv - Neuroscience 2022Quote: ... the dishes were placed on ice and 500 μl of homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) (4°C ...
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bioRxiv - Plant Biology 2022Quote: ... made up to 7.4ml with dilution buffer (10mM Tris/HCl pH 7.5, 0.5mM EDTA, 150mM NaCl, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) and stored in −80 °C until immunoprecipitation was performed.
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bioRxiv - Plant Biology 2022Quote: ... and then mixed with 4ml protein extraction buffer (50mM Tris/HCl pH 7.5, 5% glycerol, 150mM NaCl, 0.2% triton X-100, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) as soon as the ground tissue had reached room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... protein extraction buffer (50mM Tris/HCl pH 7.5 or HEPES pH 7.5, 150mM NaCl, 5% glycerol, 0.5% NP-40, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) was added to the tissue and grinding was continued until a homogenous suspension was formed ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl RNA eluate was used in a RVFV RT-qPCR using the LightCycler one-tube RNA Amplification Kit HybProbe (Roche, Almere, The Netherlands) in combination with a LightCycler 480 real-time PCR system (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl; 1 mM EDTA; 1 mM CaCl2; 1 mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] one tablet/10 mL). The cells suspension was incubated on ice for 30 min and then centrifuged at 13,000 rpm/15 min/4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and real-time qPCR reaction were carried out with KAPA SYBR FAST One-Step qRT-PCR Master Mix Kit (KAPA Biosystems, Wilmington, MA) on LighyCycler 480 system (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 M NaCl, 30 mM imidazole, 1 mM DTT, 1 mM benzamidine, and one EDTA-free complete protease inhibitor cocktail tablet [Roche] per 50 ml). The lysate was clarified by centrifugation at 38,000 g in a fixed-angle rotor ...