Labshake search
Citations for Roche :
2601 - 2650 of 8093 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for four hours at RT in AP-conjugated anti-DIG Fab fragments (Roche, 11093274910) diluted 1:2000 in 2% DIG blocking reagent ...
-
bioRxiv - Cell Biology 2024Quote: ... RT) and subsequently resuspended in 0.5 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). 0.5 ml ice-cold 8 % (v/v ...
-
bioRxiv - Pathology 2023Quote: ... JCV levels per tissue were quantified by RT-qPCR using a LightCycler® 480 instrument (Roche), the copies of JCV per reaction were multiplied by ten to obtain the copies of JCV per tissue ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The remaining RT products were collected and amplified by KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.8 µM cDNA-PCR primer ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR to assess for mRNA relative expression performed with SYBR green master mix (Roche, 04913914001) in an Applied biosciences machine ...
-
bioRxiv - Cell Biology 2023Quote: ... Each RT-qPCR reaction (10 μl) contained 5 μl of SYBR Green Select Master Mix (Roche), 300 nM of the corresponding forward and reverse primers ...
-
bioRxiv - Plant Biology 2023Quote: ... The reactions for RT-qPCR were performed using FastStart SYBR® Green Master (Roche Diagnostics GmbH) on the Rotor-Gene Q 5plex HRM Platform (QIAGEN ...
-
bioRxiv - Immunology 2024Quote: ... samples were incubated for 10 min at RT with DNAse I (50 µg/ml; Roche, 10104159001) and Dasatinib (50 nM ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: PCR was performed using the Roche High-Fidelity PCR System (Roche Life Science, Welwyn Garden City, UK). A total of 5ng DNA was amplified in a reaction volume of 10μl ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting DNA products were amplified by inverse PCR using the Expand Long Template PCR system (Roche) with primers designed within the Six2 promoter ...
-
bioRxiv - Cell Biology 2022Quote: ... and qRT-PCR was measured using gene-specific primers with FastStart SYBR Green PCR Master Mix (Roche) on a StepOne Real-Time PCR System (ThermoFisher) ...
-
bioRxiv - Genomics 2019Quote: ... and AdRb (5′ -TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16°C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65°C for 10 minutes ...
-
bioRxiv - Systems Biology 2020Quote: ... Metagenomes were obtained by pyrosequencing fragments of the 16S rRNA gene on the GS Junior platform (454 Life Sciences, Roche Diagnostics). Then the sequence data were processed by replicating the bioinformatics workflow followed by Paroni Sterbini et al ...
-
bioRxiv - Genomics 2019Quote: ... Supplementary Table S3) including 18S-28S rDNA and 5S rDNA probes were labeled with biotin-16-dUTP (Biotin Nick Translation Mix, Roche, USA) and/or digoxigenin-11-dUTP (Dig Nick Translation Mix ...
-
bioRxiv - Microbiology 2019Quote: ... 4 μl of the cDNA sample together with 16 μl mastermix were combined and analyzed using a LightCycler Nano (Roche, Germany). The light cycler program was as follows ...
-
bioRxiv - Cell Biology 2020Quote: ... and AdRb (5′-TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16° C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65° C for 10 minutes ...
-
bioRxiv - Developmental Biology 2021Quote: ... Riboprobes were hybridized for 16 hr at 65°C and embryos were incubated with anti-DIG-AP (Roche, dilution 1/4000) overnight at 4°C ...
-
bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
-
bioRxiv - Neuroscience 2020Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
-
bioRxiv - Microbiology 2021Quote: DNA protein interaction was studied with purified H-NS and DIG labeled PCR amplified PctxAB fragments by using the DIG gel shift kit (2nd Gen, Roche Aplied Science, Mannheim, Germany) as previously described (10) ...
-
bioRxiv - Physiology 2019Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
-
bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
-
bioRxiv - Genetics 2020Quote: ... Tag sequences for identifying individual trees were attached to the DNA fragments using the Access Array Barcode Library for Illumina Sequencers-384, Single Direction (Fluidigm Corporation, South San Francisco, CA, USA) and KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, Wilmington, MA, USA). PCR was performed in a 20 μL volume containing 2 μL of 20-times diluted PCR products ...
-
bioRxiv - Genetics 2022Quote: ... and amplifying the exon regions of targeted genes by using the primers with SP6 sequence (ATTTAGGTGACACTATAGAAGNG) according to the product description of KAPA HiFi PCR Kit (Roche Diagnostics, cat. no. 07958927001). Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche ...
-
bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and ligated to iTru adapters followed by limited-cycle PCR of iTru primers to add indexes and complete the library molecules using Kapa Library Preparation Kit reagents (Kapa Biosystems [Roche, Basel, Switzerland]). We sequenced the pooled libraries on an Illumina sequencer (Illumina ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were washed four times with lysis buffer complemented with 1xPIC (Roche), 20 μM DUBi (Calbiochem ...
-
bioRxiv - Developmental Biology 2024Quote: ... Beads were washed 3 times with lysis buffer containing proteinase inhibitors (Roche) and 7 times with lysis buffer without proteinase inhibitors ...
-
bioRxiv - Neuroscience 2019Quote: ... and incubated in HNPP/Fast red working solution according to manufacturer’s instructions (HNPP Fluorescent Detection Set, Roche). To stop the reaction ...
-
bioRxiv - Genetics 2019Quote: Detection of tRNA precursors was performed as described (Otsubo et al., 2018) using Digoxigenin labeled probes (Roche), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using an anti-rabbit HQ detection system (catalog # 7017936001 and 7017812001, Roche Diagnostics) followed by Chromomap DAB IHC detection kit (catalog # 5266645001 ...
-
bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
-
bioRxiv - Microbiology 2021Quote: ... Southern blot analyses were performed as previously described using the digoxigenin-anti-digoxigenin detection system (Roche Diagnostics)38.
-
bioRxiv - Neuroscience 2022Quote: ... For detection the membranes were shaken in 10 mL Lumi-light Western Blotting substrate (Roche, Cat. #12015200001) spiked in 1 mL SuperSignalTM West Femto Maximum Sensitivity Substrate (ThermoFischer ...
-
bioRxiv - Microbiology 2022Quote: ... and the reactions were run in a Light Cycler 480 II sequence detection system (Roche, Shanghai, China). The rpoC and gyrA genes were used as reference ...
-
bioRxiv - Cancer Biology 2023Quote: ... bound antibodies were detected with an anti-mouse HQ detection system (catalog# 7017936001 with 7017782001, Roche Diagnostics) and a Chromomap DAB IHC detection kit (catalog # 5266645001 ...
-
bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Detection with anti-digoxigenin antibody conjugated with alkaline phosphatase (Anti-Digoxigenin-AP Fab fragments, 11093274910, Roche, Switzerland) and activation with the chemiluminescent substrate CDP-Star (11759051001 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cDNA samples were amplified by qRT-PCR using SYBR Green PCR master mix reagents (Roche, Mannheim, Germany) in triplicate ...
-
bioRxiv - Genomics 2022Quote: ... the beads were used directly for PCR using a KAPA HiFi PCR ready mix (Roche KAPA Biosystems, Wilmington). The captured libraries from both PCR events were then combined and purified ...
-
bioRxiv - Genomics 2022Quote: ... the beads were used directly for PCR using a KAPA HiFi PCR ready mix (Roche KAPA Biosystems, Wilmington). The captured libraries from both PCR events were then combined and purified ...
-
bioRxiv - Microbiology 2024Quote: ... The lcrF transcripts of interest were detected with a DIG-labeled PCR fragment (DIG-PCR nucleotide mix, Roche) amplified with primer pairs for the individually detected according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... the mutation-containing region was amplified by PCR using the FastStart high-fidelity PCR system (Roche, Nutley, NJ) or the GC-Rich PCR system (Roche ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RT-qPCR reactions were performed on 10 ng cDNA using LightCycler 480 SYBR Green Master Mix (Roche) with 10 ul reaction volumes ...
-
bioRxiv - Microbiology 2020Quote: ... RT-qPCR reaction was performed in a Roche Lightcycler using FastStart Essential DNA Probes Master mix (Roche) and gene specific primer/probe sets (Table 1 ...
-
bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using a LightCycler 96 and the SYBR green master mix (Roche, Solna, Sweden). Gene expression levels were normalized to the AT5G09810 and AT5G25760 reference genes and displayed in relative units ...
-
bioRxiv - Plant Biology 2020Quote: ... RT-qPCR amplifications were performed from 10 ng of cDNA using SYBR Green I master mix (Roche) in 96-well plates according to the manufacturer’s recommendations ...
-
bioRxiv - Physiology 2019Quote: ... at RT followed by incubation with mouse anti-DIG alkaline phosphatase (AP) antibody (1:2000) (#11093274910, Roche) in Roche blockingbuffer (1:10 in PBT ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... One thousand ng of total RNA was used for retro-transcription (RT) (Expand™ Reverse Transcriptase, Roche). In the case of mixed infections ...
-
bioRxiv - Cell Biology 2020Quote: ... RT-qPCR to assess for mRNA relative expression was performed with SYBR green master mix (Roche, 04913914001) in Applied biosciences machine ...