Labshake search
Citations for Roche :
2401 - 2450 of 8093 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries with were multiplexed at equimolar concentration and sequenced on the Illumina HiSeq 2500 for 1×40 bases ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 µg of linearized pCR©II-Tc-mef2 or pCR©II-Tc-duf/kirre was in vitro transcribed utilizing the DIG RNA labelling kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative reverse transcription PCR was performed using Eppendorf Realplex2 Mastercycler with reaction mixtures prepared with KAPA SYBR Fast Universal qPCR Kit (KAPA Biosystems). The PCR primers for KIF1A ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Plant Biology 2021Quote: ... a digoxigenin-labeled probe corresponding to 415 bp of the ACMV AC1 gene was generated using a PCR DIG Probe Synthesis kit (Roche Diagnostics) and the primer pair ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). For PCR1 ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Zoology 2021Quote: ... a fragment of the EHP small subunit ribosomal RNA (SSU rRNA) gene was used to generate an EHP probe with a PCR-DIG labeling kit (Roche, Germany) ENF779 and ENR779 (Tangprasittipap et al. ...
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bioRxiv - Pathology 2021Quote: ... The obtained individual libraries were also quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit and an equimolecular pool of libraries were titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler 480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... Poly-A enriched libraries were generated using the Illumina TruSeq Standard mRNA preparation kit and checked via the Bioanalyzer and quantitative PCR (KAPA Biosystems). Paired-end reads (100 nt ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Indexed libraries (that met appropriate cut-offs for both) were quantified by qRT-PCR using a commercially-available kit (KAPA Biosystems), and insert size distribution was determined using a LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... the PCR products digested with indicated restriction enzymes and ligated into the pBAD28 vector using the Rapid DNA Ligation Kit (Roche Diagnostics). Inserted DNA sequences were confirmed by DNA sequencing (StarSeq).
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: PCR amplification of RNA editing containing sequence were performed by using cDNA and gDNA as templates with KAPA HiFi HotStart ReadyMix PCR kit (Roche, Germany) and the following program ...
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bioRxiv - Microbiology 2021Quote: ... were used for the purification steps and the libraries were amplified (9 amplification cycles) with the KAPA HiFi PCR Kit (Kapa Biosystems) in KAPA HiFi Fidelity Buffer (5x) ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from well-developed leaves of 14-day plants according to the modified CTAB procedure (Murray and Thompson, 1980) or by using the KAPA3G Plant PCR Kit (Kapa Biosystems). The PCR for genomic DNA isolated by CTAB was carried out in a 25 ml reaction mixture using Platinum Taq DNA Polymerase (Invitrogen ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: ... and an equimolecular pool of libraries was prepared and titrated by qRT-PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems, Fritz Hoffmann-La Roche ...
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bioRxiv - Microbiology 2019Quote: ... All q-PCRs were performed in Rotor-Gene 6000 (Corbett Life Science) and using FastStart Essential DNA Green Master Kit (Roche, Switzerland). The total volume for qPCR was 10 μl ...
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bioRxiv - Genomics 2020Quote: ... Libraries were assessed using Bioanalyzer DNA High Sensitivity chips and quantified by quantitative PCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were pooled in equimolar quantities and paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150 bases in length.
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bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
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bioRxiv - Biochemistry 2021Quote: ... the amino acid Thr at position 206 was mutated to Trp using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, USA) together with the designed complementary primers (5’- CCTATCTGATTCATGAGCACATGGTTATTTGGGATCGCATTGAAAAC-3’ and 5’- GTTTTCAATGCGATCCCAAATAACCATGTGCTCATGAATCAGATAGG-3’ ...
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bioRxiv - Genomics 2020Quote: ... The six STARR-Seq RNA replicate libraries and a STARR-Seq Plasmid DNA input library (amplified as before but with 20 ng DNA input and 15 PCR cycles) were normalized with the KAPA Library Kit (KAPA Biosystems) and sequenced on an Illumina NextSeq with 150-bp paired-end reads using standard protocols.
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription was performed on the pcr product with Ampliscribe T7-flash kit with Dig RNA labeling mix from Roche(75). In situ hybridization with both the anti-sense probes yielded the same results.
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA from twenty-four females from each of the four groups was extracted using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Microbiology 2024Quote: ... and phoD-R1083 (5’-CTG SGC SAK SAC RTT CCA-3’) (Ragot et al., 2015) in 25 µL reactions with KAPA2G Robust HotStart PCR kit (KAPA Biosystems, Roche ...
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bioRxiv - Microbiology 2024Quote: ... and phoD-R1083 (5’-CTG SGC SAK SAC RTT CCA-3’) (Ragot et al., 2015) in 25 µL reactions with KAPA2G Robust HotStart PCR kit (KAPA Biosystems, Roche; 0.5 U KAPA2G Robust HotStart DNA Polymerase ...
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bioRxiv - Developmental Biology 2024Quote: Pitx1 WISH was performed on E12.5 embryos with a digoxigenin-labelled Pitx1 antisense probe designed from a cloned antisense probe (PCR DIG Probe Synthesis Kit, Roche 11636090910). Experimental procedure followed the protocol outlined in (Kragesteen et al. ...
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bioRxiv - Genomics 2024Quote: ... Adapted fragments were amplified by 12 cycles of PCR using the Kapa Hifi Hotstart NGS library Amplification kit (Roche, Basel, Switzerland), followed by 0.8x AMPure XP (Beckman Coulter Genomics ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription and second strand synthesis was performed on the slide with cDNA quantification using qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA Biosystems) and analysed on the QuantStudio (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Systems Biology 2022Quote: ... CRISPR gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). In the first PCR step ...
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bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
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bioRxiv - Microbiology 2023Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng of DNA from each sample was analyzed using the Kapa Cyber Fast Q-PCR Kit (Kapa Biosystems, Wilmington, MA). The following rDNA primers (designed using Primer3Plus software ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 15 μL of a PCR mix with 1X LightCycler FastStart DNA Master Hybridisation Probes kit (Roche diagnostics, Mannheim, Germany), 0.5 U of UDG (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of PCR cycle used for library amplification was determined by KAPA library quantification kit for Illumina platform (KAPA biosystems). Each Hi-C library was sequenced to obtain 500M reads PE100 with biological duplicates to achieve a total of 1 B reads for each condition.
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... and an equimolecular pool of libraries was titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Genetics 2021Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2019Quote: ... and quantitative PCR (KAPA Biosystems). The libraries were clustered on 2 lanes of a flowcell then loaded on the Illumina HiSeq instrument according to manufacturer’s instructions ...