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Citations for Roche :
2501 - 2550 of 8093 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... RT qPCR was performed using the LightCycler® 480 Probes Master mix (Roche Applied Science) on a LightCycler® 480 (Roche Applied Science) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was then amplified in RT-qPCRs using LightCycler 480 SYBR Green I Master (Roche) and transcript-specific primers on a Roche LightCycler 480 thermocycler following standard protocol (45 cycles ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR assays were performed with SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat# KK4602), using primers indicated in Supplementary Table 2 ...
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bioRxiv - Bioengineering 2022Quote: ... The RT-qPCR reactions were performed using a Roche Diagnostics Lightcycler 2.0 (Roche, Indianapolis, IN), using a protocol previously described in detail42,63 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT-qPCR was performed using a Roche LightCycler® 480 System (Roche Diagnostics, Rotkreuz, Switzerland) thermocycler using qPCR MasterMix No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the LightCycler®480 SYBR Green I Master-Ki (Roche): Primers were used for GAPDH (forward ...
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bioRxiv - Plant Biology 2023Quote: ... was used to perform the RT-qPCR on a Roche LightCycler 96 thermocycler (Roche, USA). The relative gene expression levels were calculated using the 2-ΔΔCT method as described (Livak and Schmittgen ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR mix was prepared using 1X Light Cycler 480 SYBR Green Master (Roche, Germany) and primer pairs at 5uM (Table 2) ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was performed using LightCycler 480 SYBR Green I Master (Roche Life Science) with Light Cycler 96 (Roche Life Science) ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
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bioRxiv - Physiology 2022Quote: ... was performed on the cDNA using the Sybr green method of quantification on a Roche Lightcycler 480 using a SYBR FAST quantitative PCR kit (Kapa Biosystems; KK4611). Gene expression was analyzed for each sample in triplicate using the forward and reverse primers indicated on Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems, catalog #KK4605). Primers (5’3’ ...
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bioRxiv - Microbiology 2019Quote: The pooled library was prepared with 10 μl of barcoded PCR bead purified product from each individual sample and measured with KAPA qPCR library quantification kit (KAPA Biosystems, USA), before being run on an Illumina Mi-Seq Sequencer using a 600-cycle pair end reagent kit (Mi-Seq Reagent Kits v2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were subsequently dual indexed and amplified for 15 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-µl reactions following the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2019Quote: ... tail clips or seminiferous tubules were digested and PCR was performed using KAPA HotStart Mouse Genotyping Kit (KAPA BIOSYSTEMS, Cat No. KK7352). The following primer pairs were used for determining genotype-Sirt1 genotyping ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was carried out in triplicate with the LightCycler® 480 SYBR Green I Master Kit (Roche Applied Science, Penzberg, Germany) in a 15 μ L reaction on a ABI7500 (Applied Biosystems Inc. ...
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bioRxiv - Neuroscience 2020Quote: Total genomic DNA was extracted from myoblasts from all participating patients using a High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland).
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bioRxiv - Immunology 2020Quote: ... generation of TCR libraries and generation of amplicons for deep sequencing were performed using the KAPA HiFi PCR kit with GC buffer (Roche Diagnostics, #07958846001) and custom designed primers (Supplementary Table 6) ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay for size and the concentration was estimated using quantitative PCR with the KAPA Library Quantification Kit Illumina® Platforms (Roche Kapa Biosystems). To obtain sufficient amount of libraries for sequencing it was necessary for the low input libraries (0,1 - 0,2 ug ...
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Microbiology 2020Quote: Each 300 μL of GEA supernatant sample was extracted using the High Pure PCR Template Preparation Kit (Roche Life Science, Mannheim, Germany), a kit based on silica-membrane spin columns technology ...
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bioRxiv - Microbiology 2024Quote: ... while supernatants from enriched soil/water samples and rectal swabs were extracted using the High Pure PCR Template Preparation kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche, cat#07960140001). Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis and sequenced on a NovaSeq 6000 with a paired-end 150 cycle sequencing run.
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bioRxiv - Molecular Biology 2023Quote: ... For this 2 µl of RNA sample were mixed with the PCR ingredients of the one-step LightCycler 480 RNA Master Hydrolysis Probes kit (Roche Holding AG), the JFH1-specific probe (5’-6FAM-AAA GGA CCC AGT CTT CCC GGC AA-TMR-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR analysis was carried out using the qScript One-Step PCR kit (Quanta Biosciences, Gaithersburg, MD) on a LightCycler 96 System (Roche, Indianapolis, IN). Fold-change values were calculated using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2024Quote: ... A DNA library targeting polyadenylated transcripts was constructed for each sample in a 12-cycle PCR (100ng total RNA, KAPA hyper mRNA stranded library prep kit, Roche, catalogue# KK8581). Final cDNA libraries were checked for quality by TapeStation and qPCR (Kapa’s library quantification kit for Illumina Sequencing platforms ...
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free Illumina library (based on the same HMW extraction) was prepared using the Kapa Hyper Prep Kit (Roche, Basel, Switzerland), following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Biochemistry 2020Quote: ... Biotin-labeled CMV-RLuc-EV715’UTR (wild type or CCC mutant)-FLUC and biotin-labeled SL2 (wild type or CCC mutant) were synthesized using biotin-16-UTP (Roche). Binding reaction and pull-down were carried out as described5 ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was re-incubated 20 min at 75°C and the purified DNA product further incubated overnight at 16°C with a T4 DNA ligase (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Cancer Biology 2019Quote: ... The two DNA handles (510bp) labeled by multiple digoxigenin and biotin are generated by mixing biotin-16-dUTP and digoxigenin-11-dUTP nucleotides (Roche) with dNTP solution mix in the respective PCR reaction ...
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bioRxiv - Microbiology 2021Quote: We initially amplified the region of bacterial 16S-23S rRNA gene and fungi full length ITS region with Kapa Hifi DNA polymerase (Roche) and EX Taq DNA polymerase (Takara Bio) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche), and transformed into XL10-Gold E ...
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Genomics 2023Quote: ... The resulting UMI-labeled 16S dsDNA was then amplified using indexed p5/p7 primers and KAPA HiFi HotStart ReadyMix (Roche) (95°C for 3 minutes followed by 25 cycles of 98°C for 20 seconds ...
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bioRxiv - Microbiology 2024Quote: ... 5′-32P-end-labeled deoxynucleotide primer were hybridized to complementary regions of rRNA (16S nucleotides 1494–1510) and extended with AMV reverse transcriptase (Roche). Extension products were run on denaturing polyacrylamide/urea gel and visualized by autoradiography using a Typhoon PhosphorImager (Amersham Bioscience ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with Power SYBR Green PCR Master Mix (Thermofischer) on a Lightcycler 96 (Roche) with the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed by adding 15µL PCR mix containing 0.5U KAPA HiFi HotStart (Kapa Biosystems), 1x KAPA Buffer (Kapa Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany), with 4.5 mM MgCl2 and 3.5 U of enzyme mix used for each reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Standard PCR-free libraries were prepared using KAPA Hyper PCR-free library reagents (KK8505, KAPA Biosystems). Total genomic DNA was sheared into fragments of approximately 200-600 base pairs (bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems) in 20 μl reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems), 20 ng template and appropriate primers in 20 μl reactions ...