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Citations for Roche :
2801 - 2850 of 8093 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR reaction was composed of the following ingredients: 1 μl 10× FastStart PCR Buffer comprising 20 mM MgCl2 (Roche Diagnostics), 0.2 μl dNTPs (10 mM each) ...
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bioRxiv - Biochemistry 2019Quote: ... V187 were replaced by NNK codons in three rounds of inverse PCR using the Expand™ High Fidelity PCR System (Roche). One PCR reaction contained 1x Buffer 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of 4C-template DNA was used to PCR amplify the libraries using the Roche Expand long template PCR system (Roche, 11681842001). Primers were removed using SPRIselect beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... an Illumina adaptor-extension singleplex PCR step was performed using 820.000 copies of the previous PCR product with 1X KAPA HIFI HotStart ReadyMix (KAPA Biosystems KK2601), followed by double-sided (0.5X–0.8X ...
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bioRxiv - Microbiology 2024Quote: ... and CDT genes was determined by quantitative reverse transcriptase PCR (qRT-PCR) in a LightCycler 480 thermal cycler (Roche Diagnostics, USA). The primers specific for toxin A ...
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bioRxiv - Microbiology 2024Quote: ... The mRNA transcript levels of selected IVSPER genes were measured by quantitative reverse transcription-PCR (qRT-PCR) using a LightCycler® 480 System (Roche) and SYBR Green I Master Mix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4×200 ng of 4C-template DNA was used to PCR amplify the libraries using the Roche Expand long template PCR system (Roche, 11681842001) with the following PCR conditions ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in a 0.2 ml PCR tube preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM P5 transposon primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... indexes and sequencing sequences) was performed on 5 µl of purified first step PCR products using Expand™ Long Template PCR System (Roche) with the following thermocycling program ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of mRNA and pre-mRNA was then assayed by semi-quantitative reverse-transcription PCR (qRT-PCR) on a Roche LightCycler480 using SYBR Green I (Roche Diagnostics). Raw fluorescence data were analysed using LinRegPCR to perform background subtraction ...
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bioRxiv - Molecular Biology 2021Quote: ... washed two times with PBS and blocked with 1.5% blocking reagent (Roche Applied Science) at 37°C for 30 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and measured each at least 3 times (technical replicates) in the LightCycler96 (Roche, Germany). We detected expression using SYBRgreen marker (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS (supplemented with cOmplete™ EDTA-free protease inhibitors, Roche) and resuspended in 1x SDS loading dye ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed two times with cold PBS containing protease inhibitor cocktail (Roche) followed by lysis with 500 μl ChIP sonication buffer (0.5% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed 2 times with 1x PBS with protease inhibitor cocktail (Roche, 11836170001). They were lysed on the plate with cold Farnham lysis buffer to ~10×10^6 cells /mL (5mM PIPES pH 8.0 ...
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bioRxiv - Physiology 2020Quote: ... Blood glucose levels were monitored at indicated time-points using a Contour Glucometer (Roche). Mice were analyzed at ages 10 weeks (n=6-17 per group) ...
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bioRxiv - Genomics 2021Quote: ... Cells were then washed three times in ice cold PBS containing protease inhibitors (Roche), flash frozen ...
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bioRxiv - Microbiology 2021Quote: ... Hemolymph was then diluted 10 times with PBS containing Protease Inhibitor Cocktail 1X (Roche). 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Three technical replicates were prepared three times and measured on LightCycler 480 II (Roche). High-resolution melting curves were obtained by measuring the complex stability by temperature increase to 80 °C and then decrease to 20 °C with a ramp rate 0.01 °C/s in three cycles and thus obtaining a total of 27 melting curves per sample ...
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bioRxiv - Cell Biology 2022Quote: ... the calvaria were digested five times using α-MEM which contains 0.1% collagenase (Roche) and 0.2% dispase (Roche ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... A master mix was made for each qRT-PCR target containing 5 ul of KAPA Universal SYBR Fast PCR mix (KAPA Biosystems, #KK4602), 0.6ul of 5 uM forward primer ...
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bioRxiv - Microbiology 2022Quote: ... The agtA gene disruption cassette was generated by fusion PCR using an Expand High Fidelity PCR System (F. Hoffmann-La Roche, Basel, Switzerland). The 5′- and 3′-arms of agtA were amplified from wild-type A ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed in 4 replicates in final volume of 10 µL using Light SYBR Green PCR Master Mix (Roche, Applied Science) on a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments were amplified using the Kapa HiFi Hotstart Uracil+ PCR mix in 14 cycles of PCR (Kapa Biosystems, Wilmington, MA, USA). Bisulfite libraries were sequenced on an Illumina HiSeq3000 instrument (150 bp paired-end reads).
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Gut microbial disruption in critically ill patients with COVID-19 associated pulmonary aspergillosisbioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 infection was confirmed by quantitative reverse transcription PCR (TaqMan™-PCR performed on Roche cobas® 6800, Basel, Switzerland), performed on nasopharyngeal swabs or tracheal fluid ...
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bioRxiv - Molecular Biology 2023Quote: ... The relative expression levels were determined by performing a quantitative reverse transcription PCR (qRT-PCR) analysis using a LightCycler 480 system (Roche, Basel, Sweitzer) with an Evo M-MLV RT-PCR kit (Accurate Biotechnology Co. ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed with gene specific p rimers (see Key Resource Table) using Sybr green detection on a Light Cycler 480 (Roche) or Quantstudio6 (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: LDH release in the culture media from primary hippocampal neurons was measured following manufacturer instructions (Cytotoxicity Detection KitPlus –LDH, #04 744 926 001, Roche).
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies were incubated at 37°C for 1hr and Discovery multimer detection system (Discovery OmniMap HRP, Discovery DAB and Purple, Roche) was used to detect and amplify immuno-signals ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: Immunological detection was performed using antidigoxygenin-AP Fab antibody fragments at a dilution of 1:2000 (Roche Diagnostics, Mannheim, Germany). After probe hybridization ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750; Roche). The colometric reaction was performed using mixture of 5-Bromo-4-chloro-3-indolyl phosphate (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction for the internal control was performed using dual detection of FAM and YAK/VIC in a Lightcycler 480 (Roche), the detection of the N-gene was done in a QuantStudio5 cycler (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... subsequent detection by an anti-DIG antibody conjugated with alkaline phosphatase and activation with the chemiluminescence substrate CDP Star (Roche) were performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Damage to the human colon epithelial cell line HT-29 was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Developmental Biology 2022Quote: ... ISH was performed as described previously (Kraus et al., 2016) with minor changes in the RNA probe detection step: anti-Digoxigenin-AP Fab fragments (Roche) diluted 1:4000 in 0.5% blocking reagent (Roche ...
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bioRxiv - Microbiology 2019Quote: Damage to the human colon epithelial cell line HT-29 and macrophages J774A.1 (ATCC TIB-67) were assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of LDH in the growth medium ...
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... release into growth medium (as direct indicator of cell membrane integrity loss) was performed with the Cytotoxicity Detection KitPLUS (Roche) according to the supplier’s manual.
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bioRxiv - Cancer Biology 2020Quote: ... Incubation with the appropriate secondary HRP-labeled antibody was followed by detection with ECL western blotting substrate (Roche Applied Science).
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bioRxiv - Pathology 2021Quote: ... Quantitative PCR (qPCR) assay was performed by using the SYBR qPCR Master Mix (Vazyme) on Roche Lightcycle480 II Sequence Detection System (Roche). Gene expression levels were normalized by Actb ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Microbiology 2023Quote: ... and the murine J774A.1 (ATCC TIB-67) macrophages was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridization of the DIG-telomere probe and chemiluminescent detection was performed according to the protocol from the TeloTAGGG Telomere Length Assay (Roche). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were detected using horseradish peroxidase-conjugated and detection with the Lumi- Light Plus Western Blotting Substrate (Roche). Luminescence was detected with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using an anti-rabbit HQ and anti-HQ HRP detection system (Roche, catalog 7017812001 and 7017936001) followed by Chromomap DAB IHC detection (Roche ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed using KAPA HiFi (Roche KK2101) for all amplifications with plasmid or phage templates ...
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bioRxiv - Genetics 2021Quote: ... PCR with Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) was performed on the cDNA using M28 virus-specific primer pairs ...