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Citations for Roche :
2501 - 2550 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
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bioRxiv - Physiology 2021Quote: ... at time zero (T0) peripheral glucose level was measured at the tail using a One-Touch ultra-glucometer (AccuChek Sensor, Roche Diagnostics) and at T15 ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl, 20 mM Imidazole pH 8.0, one tablet of EDTA-free protease inhibitor [Roche; Product # COEDTAF-RO], DNaseI [Roche; Product #11284932001]) and lysed and centrifuged for 30 minutes at 18,000 x g at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... one lobe of dissected adipose tissue was homogenized with a Teflon pestle in RIPA buffer containing protease (cOmpleteTM Mini, Roche, Germany) and phosphatase (5 mM Na fluoride ...
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bioRxiv - Physiology 2024Quote: Blood glucose levels were measured immediately in one drop of blood after the heart puncture with the Blood Glucose System Accu-Chek Performa (Roche Diagnostics).
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... one half was immunoblotted with NB13 (7 µg/mL) followed by washing and probing with α-HA-peroxidase (1:1000, Roche), whereas the other half was probed with α-His-peroxidase only (1:4000 ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM sodium dithionite) supplemented with 0.2 mg/mL bovine pancreatic deoxyribonuclease I and one cOmplete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were centrifuged and the pellet was resuspended in Resuspension Buffer (25 mM Tris HCl pH 7.5, 250 mM NaCl) supplemented with one tablet of cOmplete protease inhibitor (Roche, Cat # 04693159001). Cells were then lyzed using a high-pressure Emulsiflex C5 homogenizer (Avestin) ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM imidazole) supplemented with DNase and one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche #11 873 580 001). Cells were lysed using a French press (2x ...
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bioRxiv - Immunology 2021Quote: ... followed by an overnight incubation at 65°C with Proteinase K (Roche). DNA was purified with the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... were treated at 37 °C by Proteinase K (3.8 μg/ml) (Roche). Aliquots were removed at 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were cooled to 55°C and PCR grade Proteinase K (Roche) was added to a concentration of 1 ug/ul ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at 37°C followed by Proteinase K (Roche 03115887001) for 1 hour at 50°C ...
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bioRxiv - Neuroscience 2021Quote: ... 30°C in Kreb’s buffer with 10 μg/ml adenosine deaminase (Roche). Pharmacological treatments included CP681301 (provided by Pfizer) ...
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bioRxiv - Molecular Biology 2021Quote: ... which had been pre-treated with 8 μg/ml mitomycin C (Roche). Throughout the immortalization process ...
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bioRxiv - Bioengineering 2020Quote: ... and digested enzymatically at 37 °C with Liberase TM (Roche, Indianapolis, IN). Supernatants from six successive digestion periods were collected ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were detected with Anti-c-myc-Peroxidase (Roche #11814150001) at dilution of 1:5,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... reverse crosslinking (65°C overnight in the presence of Proteinase K (Roche)) was followed by two sequential phenol/chloroform/isoamylalcohol extractions ...
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bioRxiv - Genomics 2019Quote: ... were bound to nuclear extract prior to poly d(I-C) (Roche) incubation at 4°C for 1 hr.
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bioRxiv - Biophysics 2019Quote: ... in the presence of 50 ng/ul poly-d(I-C) (Roche) and with 20 ul total volume ...
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bioRxiv - Genetics 2020Quote: ... acid-extracted histones were digested with Asp-N and Arg-C (Roche) and the resulting digests were analyzed by chromatography on an Ultimate 3000 nanoLC (Dionex ...
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bioRxiv - Molecular Biology 2021Quote: ... which had been pre-treated with 8 μg/ml mitomycin C (Roche). Throughout the immortalization process ...
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bioRxiv - Developmental Biology 2021Quote: HEK293T cells were cultured at 37°C and transfected with Fugene (Roche). 48 h post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... or monoclonal c-myc antibody (Roche, used at a 1/10000 dilution). Polyclonal and monoclonal antibodies were detected by goat anti-rabbit or anti-mouse IgG coupled to peroxidase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Hi-C libraries were prepared using KAPA LTP Library Preparation Kit (Roche) 65 with 12 amplification cycles ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Cancer Biology 2023Quote: ... Embedded slides were paraffinized at 72°C with EZ solution (Roche, Ventana) and MECOM antigen was retrieved with CC2 pre-diluted Tris solution for 64 minutes at 91℃ ...
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bioRxiv - Plant Biology 2024Quote: ... at the annealing temperature of 58 °C on LightCycler480 instrument (Roche, Switzerland). PCR efficiency was estimated using serial dilution of template cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Tokyo Japan) using an anchored-oligo(dT)18 primer and random hexamer primer ...
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bioRxiv - Cell Biology 2021Quote: ... of these dilutions were used to prepare the reaction mixes for the real-time qRT-PCR using the KAPA SYBR FAST One-Step Universal kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of the total RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Florham Park, NJ). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Each region was amplified from 5 μl of the RNA preparation by RT-PCR using Transcriptor One Step RT-PCR kit (Roche Applied Science). To perform the RT-PCR ...
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bioRxiv - Biochemistry 2021Quote: The pupae infected by the baculovirus were homogenized with a buffer solution (20 mM Tris-HCl, 150 mM NaCl, 5 mM DTT, 1mg/ml phenylthiourea, one inhibitor cocktail tablet (EASYpack, Roche, Basel, Switterland) per 50 ml solution ...
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bioRxiv - Molecular Biology 2021Quote: RNA template (500 ng) was used for one-step RT-PCR amplification protocol (SYBR Green I RNA amplification kit, Roche, Basel, Switzerland). Primers were designed using the LC Primer/Probe design software (Roche ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was added to optimized 10 µM primer/probe mix in Mastermix (Quantabio, qScript™ XLT One-Step RT-qPCR ToughMix®) and run on StepOne Plus real-time PCR (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: The Illumina library was prepared with 250 ng of unsheared DNA using the Kapa Hyperplus Library Preparation Kit with only one third of the volume reactions as described in the manufacturer’s protocol (Kapa Biosystems, Wilmington, MA). The total fragmentation volume was 16.66 μl and was incubated at 37°C for 7:45 min ...
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bioRxiv - Microbiology 2023Quote: One microgram of RNA of each isolate was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). RT-PCR was performed in 96-well plates using the PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... For this 2 µl of RNA sample were mixed with the PCR ingredients of the one-step LightCycler 480 RNA Master Hydrolysis Probes kit (Roche Holding AG), the JFH1-specific probe (5’-6FAM-AAA GGA CCC AGT CTT CCC GGC AA-TMR-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... The bulk transcriptome of these cases was obtained after macrodissection of the whole tumor area on the same block as the one used for the IHC and obtained from FFPE sections using a high-purity FFPE RNA isolation kit (Roche, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR analysis was carried out using the qScript One-Step PCR kit (Quanta Biosciences, Gaithersburg, MD) on a LightCycler 96 System (Roche, Indianapolis, IN). Fold-change values were calculated using the 2-ΔΔCt method ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was reverse transcribed using a TransScript Uni All-in-One (TransGen Biotech, Beijing, China) and reacted with specific primers using a FastStart Universal SYBR Green Master Mix (ROX) (Roche, Switzerland, Basel) and Bio-Rad CFX96 (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 5-bromo4-chloro-3-indolyl phosphate toludinium (Roche Diagnostics, Cat. No 11383221001). Images were taken on a Zeiss Axio Compound Light Microscope with Optronics MacroFire Digital Camera.
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bioRxiv - Developmental Biology 2021Quote: ... 3 % SDS with protease inhibitors (cOmplete Mini EDTA-free Protease Inhibitor Cocktail, Roche) at room temperature for 1 hour in a total volume of 3 mL ...
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bioRxiv - Microbiology 2021Quote: ... 3 mM DTT and 1 mM PMSF) supplemented with protease inhibitor cocktail (Roche). Zirconium beads equivalent to 100 µL volume was added in microcentrifuge tubes and resuspended cells were lysed by 6 rounds of bead beating on a bullet blender ...
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bioRxiv - Cell Biology 2022Quote: ... collected in a 1.5 ml tube and blocked overnight in 3% BSA (Roche) before incubating in primary antibody (3% BSA in PBT ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T?cells using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’ and 3’ linkers were terminally labelled with digoxigenin-11-dUTP (Roche) or biotin-16-dUTP (Roche) ...