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Citations for Roche :
201 - 250 of 7968 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Humanization of the heavy (Vh) and light (Vl) chains of the best two candidates was performed by Roche using proprietary technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Physiology 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green protocols (Kapa Biosystems, MA, USA) on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system) ...
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bioRxiv - Physiology 2023Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was carried out in a LightCycler 480 detection system (Roche) using the LightCycler FastStart DNA Master plus SYBR Green I kit (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (RT-qPCR) analyses were performed using a FastStart universal probe master (Roche) and StepOnePlus PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Developmental Biology 2023Quote: ... All genotyping reactions were performed with KAPA HotStart Mouse Genotyping Kit (Roche, 07961316001) with 1 μL of gDNA and a final primer concentration of 5 μM each ...
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bioRxiv - Developmental Biology 2023Quote: Probes for whole mount ISH and FISH were prepared with RT-PCR using primers listed in Table S7 followed by ligation into pGEM-T Easy vectors and transcribed using a DIG RNA labeling kit (Roche, Cat no. 11175025910), some probes were made using flourescein labeling mix (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... cells were mechanically unroofed at 37°C using distilled water containing protease inhibitors (Roche) and 10 μg/mL phalloidin (Sigma-Aldrich P2141 ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Plant Biology 2019Quote: ... blocked in 1×TBS-T with 1% Blocking Reagent (Roche) at room temperature for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... A 20 mM stock solution of T-20 (enfuvirtide; Roche) was prepared in H2O and stored at −20°C ...
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bioRxiv - Microbiology 2022Quote: ... 50 µM T-20 (Enfuvirtide; Roche; stock solution 20 mM) was added to prevent second-round infection ...
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Microbiology 2019Quote: ... each DNA sample was amplified by polymerase chain reaction (PCR) using KAPA HiFi HS ReadyMix (KAPA Biosystems, Wilmington, MA) and primers specific for variable regions 3 and 4 of the 16S rRNA gene ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was measured by quantitative Real Time - Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics, Basel, Switzerland) using the SYBR Premix Ex Taq (Tli RNaseH ...
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bioRxiv - Microbiology 2022Quote: ... Measurement of viral DNA was performed using a quantitative polymerase chain reaction (qPCR) assay (Cobas 6800; Roche, Basel, Switzerland).
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to the manufacturer’s instructions and quantified by real-time quantitative polymerase chain reaction (RT-qPCR) performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Physiology 2023Quote: ... and quantitative real-time polymerase chain reaction (RT-PCR) was performed with SYBR Green protocols (Kapa Biosystems, MA, USA) and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative polymerase chain reactions (qPCRs) were performed using LIGHTCYCLER 480 SYBR GREEN I Master Mix (Roche, Indianapolis, IN, USA) in a Bio-Rad CFX384 Touch Real-Time PCR Detection System with 10 µL reaction volume ...
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bioRxiv - Systems Biology 2022Quote: ... barcoded region was amplified using the following primers and 2x KAPA HiFi Hotstart ReadyMix (Kapa Biosystems, Cat#KK2602) which yielded ~350 bp product ...
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bioRxiv - Microbiology 2021Quote: ... A 1 uL aliquot was used to amplify the V1-V3 region using HiFi HotStart ReadyMix (KAPA BioSystems) and the Illumina PCR protocol ...
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bioRxiv - Immunology 2022Quote: ... 16S rRNA V3 and V4 regions were amplified by PCR using Kapa Hifi Hotstart Ready Mix (KAPA Biosystems) with an amplicon PCR primer set (Forward ...
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bioRxiv - Cancer Biology 2024Quote: ... the mutation-containing region was amplified by PCR using the FastStart high-fidelity PCR system (Roche, Nutley, NJ) or the GC-Rich PCR system (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The regions of interest (Table S1) were enriched using SeqCap HyperChoice Library probes (NimbleGen; Roche Diagnostics, Jakarta, Indonesia). Libraries were sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: in situ Cell Death Detection Kit (Roche) was used for evaluation of cell apoptosis.
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bioRxiv - Cell Biology 2019Quote: ... Splenocytes were fused with PAI mouse myeloma cells using polyethylene glycol (Roche). Hybridoma supernatants were screened by indirect ELISA with His-tagged Sec23A as the antigens ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were first pre-incubated with 2.4G2 antibody (kindly provided by Louis Boon) for blocking Fc receptors and next stained for surface markers in PBS supplemented with 0.5% BSA (Roche) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Tail genomic DNA was extracted using a KAPA mouse genotyping Kit (KAPA Biosystems KK7352), and PCR was performed using primers as described in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted from tail tissue with a KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... DNA was extracted from tail tissue using the KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping was performed via tail clips using the KAPA Mouse Genotyping Kit (Roche #07961766001). See Table S2 for primer sequences ...
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bioRxiv - Biochemistry 2023Quote: ... Glu-C (Roche), and chymotrypsin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Lys-C (Roche), Glu-C (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 10 min at 37°C with anti-HA antibody (3F10; Roche) (1:3000 ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed at 4°C in RIPA buffer supplemented with protease inhibitors (Roche, 4693159001). Protein concentrations were measured using a BCA Assay Kit (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... was incubated for 30m at 4 °C and detection was achieved using UltraView DAB detection kit (Roche). Protein expression was scored by a board-certified pulmonary pathologist blinded to cohort status or treatment group ...
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bioRxiv - Cancer Biology 2022Quote: Engineered monoallelic cell pellets (500 million cells/sample) were lysed at 4°C in 1% CHAPS (Roche Diagnostics, cat no. 10810126001) lysis buffer (pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Biochemistry 2022Quote: ... I analyzed samples by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) using a Light Cycler 1.5 (Roche Diagnostics). Measurements form RNA of vascular cell adhesion molecule-1 (VCAM-1 ...
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bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription polymerase chain reaction (RT–PCR) was performed on the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using the MTT cell proliferation kit I (Roche), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... cell viability was measured by the MTT assay (Cell proliferation kit, Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Developmental Biology 2021Quote: Injected tissue was isolated from the thoracic region of embryos and lysed with Radio Immunoprecipitation Assay (RIPA) buffer (Roche). Total protein concentration was measured using a DC Protein Assay kit (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: PCR-based amplification of sgRNA regions was performed as described previously27 with minor modifications - KAPA HiFi HotStart ReadyMix (Roche) was used for PCR1 and PCR2 with primers listed in Table S2 and following cycling conditions ...