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Citations for Roche :
301 - 350 of 7968 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Zoology 2019Quote: ... Polymerase Chain Reaction (PCR) amplification was conducted in 12.5 μl reaction volumes consisting of AmpliTaq® DNA polymerase (Roche Molecular Systems, Inc) forward and reverse primers (0.5 μM each) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) analysis of cDNA expressions was conducted using SYBR FAST Universal qPCR Master Mix (Kapa Biosystems; #KK4602) with a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: 293T cells were co-transfected with plasmids encoding cognate heavy and light chains using the Xtreme Gene 9 DNA transfection reagent (Roche, Basel, Switzerland), then grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were quantified by Reverse Transcription quantitative polymerase chain reaction (RT–qPCR) using the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland). Cycling parameters were set at (i ...
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bioRxiv - Microbiology 2023Quote: ... Abutting primer pairs were designed that were specific to these genetic groups (Supplementary Table 3) to enable the recovery of full viral genomes using polymerase chain reaction (PCR) with HiFi HotStart DNA polymerase (Kapa Biosystems, USA) using cycling conditions per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT– PCR) was performed in optical 96-well plates via the LightCycler 480 II system (Roche Life Science) using KOD SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Neuroscience 2021Quote: ... For TUNEL staining (In Situ Cell Death Kit, Roche), fresh frozen transverse cryostat sections (30 μm ...
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bioRxiv - Neuroscience 2022Quote: ... In Situ Cell Death Detection kit (Roche Cat# 11684795910) was used to assess cell death.
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bioRxiv - Microbiology 2024Quote: ... the Cell proliferation Kit II (XTT) (Roche, Basel, Switzerland) was used ...
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bioRxiv - Cell Biology 2019Quote: ... The apoptotic cells were defined by using In Situ Cell Death Detection Kit (Roche) following manufacturer’s protocol and quantified by calculating positively stained cells in at least five randomly chosen HPFs of each slide.
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bioRxiv - Microbiology 2021Quote: ... Apoptotic cells were quantified by the In situ cell death detection kit (12156792910, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Relative cell viability was assessed using the XTT cell proliferation Kit II (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Apoptotic cells were visualized using the In Situ Cell Death Detection Kit (Sigma/Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
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bioRxiv - Microbiology 2019Quote: ... Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Purified DNA from mouse fecal pellets or sorted cells was subjected to 16S variable region 4 PCR amplification using barcoded 515F and 806R primers (47) and the KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), with the following cycling conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Biochemistry 2019Quote: ... Blocking reagent for ELISA (BRE) and cOmplete protease inhibitor tablets were from Roche (Basel, Switzerland). Phosphate buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: ... 100uL of the clarified supernatant was used in the anti-BrdU DNA fragmentation ELISA (Roche). Each condition was tested in technical triplicates ...
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bioRxiv - Neuroscience 2021Quote: ... NeuroMab; HCN2: mouse, 75-111, NeuroMab; HCN3: mouse, 75-175, NeuroMab; HCN4: mouse, 75-150, NeuroMab; HA: mouse, 12CA5, Roche) in 1% milk powder and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Accu Check® Safe-T-Pro Plus lancing device (Roche AG, Switzerland, Basel) was employed to prick the eggs ...
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bioRxiv - Neuroscience 2023Quote: ... alpha synuclein and NfL) were measured in both cohorts using Elecsys assays in accordance with the manufacturer’s instructions (Roche Diagnostics International Ltd).52 CSF analyses were performed by technicians blinded to all clinical and imaging data ...
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bioRxiv - Neuroscience 2020Quote: DNA was extracted from mouse ear biopsies using High Pure PCR Template Preparation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: DNA was extracted from tail or ear clippings using the Kapa Mouse Genotyping Standard kit (KAPA Biosystems) and stored at -20°C ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspension was then chemically digested by incubation at 37°C for 30 min with collagenase (Roche #11088866001), dispase (Stemcell Technologies #07923 ...
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bioRxiv - Cell Biology 2023Quote: ... or MDCK-Ecad Res cells were pre-treated with Mitomycin C at a concentration of 10μg/ml (Roche) for a duration of 1 hour to inhibit cell proliferation ...
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bioRxiv - Immunology 2024Quote: ... followed by a subsequent incubation at 95 °C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics, Canada). Following this ...
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bioRxiv - Immunology 2021Quote: ... Mouse (Roche) on the LightCycler 2.0 (Roche) ...
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bioRxiv - Bioengineering 2019Quote: ... For molecular cloning 3 µg of RNA were reverse transcribed for 30 min at 50°C using an oligo(dT) primer and the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) followed by 5 min enzyme inactivation at 85°C according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Cell Biology 2022Quote: mRNA was purified from ∼200 ng of total RNA with oligo-dT beads and sheared by incubation at 94°C in the presence of Mg2+ with the KAPA mRNA HyperPrep kit (Roche). Following first-strand synthesis with random primers ...
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bioRxiv - Immunology 2020Quote: ... Telomere dot blot was performed by 3h hybridization at 42°C with DIG-labelled telomere probe as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). Control Input was 200 ng genomic DNA from APCs ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Neuroscience 2019Quote: ... Genotyping of the OXTR SNPs was performed by real-time polymerase chain reaction (PCR) and subsequent melting curve detection using a Cobas Z 480 Light Cycler (Roche Diagnostics, Mannheim, Germany). With the melting curve analyses ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping was conducted by real-time polymerase chain reaction (RT-PCR) and subsequent high resolution high-resolution melting on a Cobas Z480 Light Cycler (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... A quantitative real time polymerase chain reaction (q-RT-PCR) was used to quantify the parasite load by using PCR SYBER Green Master Mix (Roche, Applied Science, CT) containing MgCl2 by employing QuantStudio 3 Real-Time PCR system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell survival relative to vehicle-treated cells was assessed using the WST-1 kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: The cell proliferation assay was carried out using WST-1 Cell Proliferation Assay Kit (Roche). Transfected cells were seeded into 96-well plates at a density of 1 × 103 cells/well ...
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bioRxiv - Cell Biology 2021Quote: Apoptotic cell analysis was performed using an in situ Cell death detection Fluorescein kit (Roche). Formalin-fixed ...
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bioRxiv - Cell Biology 2022Quote: ... cell viability was determined using the XTT cell proliferation kit II (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cell death was assessed using TUNEL staining from In Situ Cell Death Detection Kit (Roche) with Opal-based costain for sarcomeric actin as described above ...
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bioRxiv - Cell Biology 2024Quote: Cell viability staining was performed using the In situ cell death detection kit (Roche Diagnostic). In summary ...
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bioRxiv - Physiology 2023Quote: ... Cell death was detected using an In Situ Cell Death Detection Kit (Roche, catalog # 12156792910). CD63 and ALP double positive (CD63+ ...
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bioRxiv - Neuroscience 2023Quote: ... cell viability was measured using the Cell Proliferation Kit I (MTT) (Roche Applied Science, CH) according to manufacturer’s protocol ...