Labshake search
Citations for Roche :
51 - 100 of 7968 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... The region around the E-Box was amplified with primers outside of the homology regions using Kappa HiFi HotStart ReadyMix (Roche) and cloned into pCR-Blunt II-TOPO (Thermo Fisher) ...
-
bioRxiv - Microbiology 2021Quote: We initially amplified the region of bacterial 16S-23S rRNA gene and fungi full length ITS region with Kapa Hifi DNA polymerase (Roche) and EX Taq DNA polymerase (Takara Bio) ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissues were washed three times with TBS-T and were incubated with a TUNEL reaction mixture (In Situ Cell Death Detection Kit, TMR red, Roche 12156792910) for 1 h at 37 °C ...
-
bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
-
bioRxiv - Cancer Biology 2019Quote: Quantification of libraries was performed by quantitative polymerase chain reaction (qPCR) using the KAPA Library Quantification kit (KAPA Biosystems). Library fragment size was determined by the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The cell proliferation ELISA BrdU (5′-bromo-2′-deoxyuridine) colorimetric assay (Roche,11647229001) was performed as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: Apoptosis in human islets was determined using Cell Death Detection ELISA (Roche, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: BAM files from previously published multi-region WES data (generated using the SeqCapEx Exome Enrichment Kit v3.0 (Nimblegen/Roche)) from nine sporadic fresh-frozen adenomas were available (Supplementary Table 2).11 Somatic variants were re-called using Mutect2 as above.
-
bioRxiv - Microbiology 2020Quote: ... and via real-time quantitative polymerase chain reaction (qPCR) with the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). Final library pools were spiked with a non-indexed PhiX control library (Illumina ...
-
bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
-
bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
-
bioRxiv - Bioengineering 2021Quote: ... 700 µl of T cell media + 30 U/ml rhIL-2 (Roche) was added to each well ...
-
bioRxiv - Immunology 2022Quote: ... CAT expression was determined using an ELISA kit in accordance with the manufacturer’s instructions (Roche). Bovine IFN-α standards were used to construct a type I IFN standard curve to interpolate sera sample IFN levels.
-
bioRxiv - Microbiology 2020Quote: ... CD4+ T cells and B cells were activated for 48h with 10U/ml IL-2 (Roche) and 2 μg/ml phytohemagglutinin (PHA ...
-
bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
-
bioRxiv - Molecular Biology 2021Quote: Serum albumin measurements were determined using a commercial serum albumin kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on Cobas Mira analyser (Roche Diagnostics Ltd ...
-
bioRxiv - Cancer Biology 2022Quote: The BrdU assay was performed using the Cell Proliferation ELISA BrdU assay (Roche #11647229001) according to manufacturer ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cellular proliferation was assessed with the BrdU cell proliferation ELISA (11647229001, Roche, Basel, Switzerland). In brief ...
-
bioRxiv - Immunology 2022Quote: ... measured by RT ELISA (Roche), per well (MOI 0.2 ...
-
bioRxiv - Genomics 2023Quote: ... ELISA BrdU Colorimetric assay (Roche) was performed according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Using the transcriptor first-strand cDNA synthesis kit with oligod(T) primers (04897030001; Roche Molecular Biochemicals ...
-
bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
-
bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
-
bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
-
bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
-
bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with the KAPA SYBR Fast qPCR kit (KAPA Biosystems Cat# KK4608) on a CFX96 Real-Time PCR Detection System (Bio-Rad RRID ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 μL of whole cell extract (WCE) was incubated overnight at 4°C with 2 μg anti-c-myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins ...
-
bioRxiv - Immunology 2020Quote: Telomerase activity was assessed with a TeloTAGGG telomerase ELISA kit according to the manufacturer’s instructions (Roche) and extracts of 2 × 103 viable T cells as described previously27.
-
bioRxiv - Molecular Biology 2022Quote: ... DNA fragmentation was evaluated using a Cellular DNA Fragmentation ELISA Kit (Roche Applied Science, Mannheim, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: Quantitative Polymerase Chain Reaction (qPCR) was performed on a LightCycler480 (Roche) using SYBR Green I master mix (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
-
bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
-
bioRxiv - Cell Biology 2023Quote: ... KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352) were used to identify genotypes of mice.
-
bioRxiv - Microbiology 2020Quote: ... Pegylated interferon alpha-2a (PEG-IFN-α; Pegasys, 90 mcg, Roche) was aliquoted and stored at room temperature until further use ...
-
bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
-
bioRxiv - Microbiology 2019Quote: ... The V3-V4 region of the 16S rRNA gene was PCR amplified using the KAPA HotStart PCR Kit (Kapa Biosystems, Wilmington, MA) with 10 µL Kappa HotStart Mastermix ...
-
bioRxiv - Microbiology 2023Quote: ... a 710 bp region was amplified by PCR using previously described primers (45) and the KAPA HiFi HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
-
bioRxiv - Plant Biology 2020Quote: ... and identified by morphological features.41 Polymerase chain reaction (PCR) products were purified with the High Pure PCR Product Purification Kit (Roche Diagnostics, Mannheim, Germany), and sequenced in both directions by Macrogen Inc ...
-
bioRxiv - Genomics 2020Quote: ... Library concentrations were determined by quantitative polymerase chain reaction (qPCR) using the KAPA SYBR FAST ABI Prism qPCR Kit (Kapa Biosystems, Wilmington, MA) and the StepOnePlus Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: Measurement of BrdU incorporation was analyzed using the Cell Proliferation ELISA BrdU colorimetric test (#11647229001, Roche), in line with the manufacturer’s instruction ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Quantitative polymerase chain reaction (qPCR) were performed in a LightCycler 480 (Roche) using the GoTaq® qPCR Master Mix kit (Promega ...
-
bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
-
bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
-
bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
-
bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... and AT5G57785 upstream regions were PCR amplified by KAPA Hifi polymerase (Roche) and cloned into an Oryza sativa H2B-VENUS fusion construct created by gene synthesis (Twist Bioscience) ...