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Citations for Roche :
151 - 200 of 7968 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Quantitative polymerase chain reactions (qPCRs) were performed using LIGHTCYCLER 480 SYBR GREEN I Master Mix (Roche) either using 20 μL reaction volume in a LightCycler® 480 System II (Roche) ...
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bioRxiv - Immunology 2020Quote: ... quantitative polymerase chain reaction (RT-qPCR) was performed with SYBR FAST qPCR Master Mix (Kapa Biosystems) with primers shown in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2023Quote: Polymerase chain reaction: 240 μL of the PCR supermix (1.66x KAPA HiFi HotStart readymix (Roche, 07958935001), 1.33 μM PCR primer ...
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bioRxiv - Developmental Biology 2021Quote: HEK293T cells were cultured at 37°C and transfected with Fugene (Roche). 48 h post transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR analyses confirmed mouse genotypes with DNA isolated from ear biopsies using the KAPA Mouse Genotyping Kits (Kapa Biosystems). All PCR primers are listed in Supplementary Table 1.
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...
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bioRxiv - Immunology 2020Quote: ... These were identified by immunohistochemical staining (IHC) with antibodies against T cells: CD3 (Roche Cat # 790-4341, pre-diluted), CD4 (Roche ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Molecular Biology 2019Quote: ... and CAT expression was quantified using the CAT ELISA (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Reagent for ELISA (BRE) (11112589001) was manufactured by Roche. 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking reagent for ELISA (BRE, cat. 11112589001) was from Roche. DC Assay kit was from Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Plant Biology 2020Quote: ... roots were incubated overnight at 4°C in anti-myc-mouse primary antibody (1:250, Roche in blocking solution), washed 3 times in PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Immunology 2022Quote: ... The ITS1 region was amplified using 2X KAPA HiFi Hotstart ReadyMix following manufacturer’s recommendations (Roche, UK), with 0.4 μM primers ...
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bioRxiv - Molecular Biology 2022Quote: ... One set specific to the GFP region includes the Universal probe library (UPL) probe #67 (Roche), the forward primer ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Bioengineering 2020Quote: Quantitative Polymerase Chain Reactions (qPCR) were prepared in 10μ reactions with SYBR Green I Master Mix (Roche), 1μL of genomic extract as a template ...
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bioRxiv - Cell Biology 2019Quote: ... The COMMD1 sequence was amplified by polymerase chain reaction using the Pfu DNA polymerase (Roche, Basel, Switzerland), using an upstream primer to add a HindIII restriction site and retaining the SalI downstream of the coding sequence ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The RBD fragment was amplified via polymerase chain reaction using Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) and then Gibson assembled in frame with an N-terminal His6-SUMO tag in the same vector as above ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using SYBR green PCR master mix (Kapa biosystems), forward and reverse primers (200 nM) ...
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bioRxiv - Biochemistry 2024Quote: ... excluding mitochondrial targeting sequence) were amplified by polymerase chain reaction (PCR) using Kapa HiFi Hotstart ReadyMix (Roche) and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase Chain Reactions (PCR) were performed using KAPA HiFi HotStart Ready Mix® (KAPA biosystems, Boston, USA) with the following thermal parameters ...
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bioRxiv - Plant Biology 2024Quote: ... Gene expression was measured by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics) using the SYBR Premix Ex Taq (Tli RNaseH ...
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bioRxiv - Genetics 2024Quote: ... TaqMan real-time polymerase chain reaction assays (PCR) consisted in 2.5 ul of Fast Master Mix (Roche), 0.125 ul of assay ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysate was incubated with agarose beads conjugated with either mouse IgG or mouse anti-GFP antibodies (Roche) for 3h at 4°c under gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... Genotyping of mice was performed using a mouse genotyping kit (Kapa Biosystems). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Genotyping of mice was performed using a mouse genotyping kit (Kapa Biosystems). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... P14 CD8+ T cells were activated for 24 h as described above and resuspended in T cell media + 30 U/ml rhIL-2 (Roche) at 2 × 106 cell/ml ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated for 1 hour at 37°C with 10U DNase (Roche) in R10 medium (RPMI 1640 medium containing GlutaMAX from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... RNA was reverse transcribed with pd(T)-15 (Roche) and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... 30 μl/well of BM Chemiluminescence ELISA substrate (Roche, 1:50) was added to the plate ...
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bioRxiv - Developmental Biology 2022Quote: ... cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche, 11 175 025 910). Images were taken on an SPE Leica confocal microscope and processed using FIJI and Adobe Photoshop programs.
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-Green Fluorescent Protein overnight at 4°C (Clones 7.1 and 13.1; 1:50 dilution, Roche, Mannheim, Germany) and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... lower and upper mouse and hamster first molars were dissected as above and treated for 15 minutes at 37°C with Dispase (Roche) 10mg/ml in Hepes/KOH 50mM ph7.7 ...
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bioRxiv - Molecular Biology 2021Quote: ... cell viability was assessed using Cell Proliferation Kit I (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of heavy- and light chain pDNA were mixed with X-tremeGENE HP DNA Transfection Reagent (Roche) in FreeStyle™ 293 Expression Medium (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Quantitative polymerase chain reaction (qPCR) was carried out using SYBR green mix buffer (Roche Applied Science, Meylan, France) in a LightCycler 480 Real-Time PCR System (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was conducted on Roche Light cycler®96 (Roche Diagnostics, Mannheim, Germany) and amplification curve was checked with ROCHE software version 1.1 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using KAPA SYBR FAST qPCR Master Mix (KM4101, KAPA Biosystems, USA). The 2-ΔΔCT method was used for quantitative analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Humanization of the heavy (Vh) and light (Vl) chains of the best two candidates was performed by Roche using proprietary technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Physiology 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green protocols (Kapa Biosystems, MA, USA) on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system) ...
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bioRxiv - Physiology 2023Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was carried out in a LightCycler 480 detection system (Roche) using the LightCycler FastStart DNA Master plus SYBR Green I kit (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (RT-qPCR) analyses were performed using a FastStart universal probe master (Roche) and StepOnePlus PCR system (Applied Biosystems) ...