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Citations for Roche :
2551 - 2600 of 8008 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at RT with the secondary antibodies together with DAPI (1:5,000; Roche), all diluted in Dako Antibody Diluent ...
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bioRxiv - Developmental Biology 2023Quote: ... R-5’-GGGACGCAGCAACTGACATT-3’) was assessed by RT-qPCR using SyBR Green solution on a LightCycler480 (Roche). The cDNAs of every single cell were purified using the DNA Clean & Concentrator Kit (Zymo ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of RNA was used for reverse transcription (RT) using Transcriptor universal cDNA master mix (Roche) for Min6 cells or superscript III enzyme (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RT–qPCR was performed on a LightCycler 480 system using LightCycler SYBR Green I Master (Roche Diagnostics). The primers were designed with the software Primer 3 ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplifications were conducted using Pwo (Roche) and GoTaq (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 1X PCR DIG labeling mix (Roche, USA), 1.5 mM MgCl2 ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed using SybrGreen (Roche) on a Roche480II Lightcycler using the following primers ...
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bioRxiv - Bioengineering 2019Quote: ... Expand High Fidelity PCR system (Roche Diagnostics) was used for PCR amplifications according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 1M Resolution Solution (GC-RICH PCR, Roche) was added to each qPCR reaction (47) ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed on LightCycler480II (Roche) using the KAPA-SYBR-FAST qPCR kit (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed in a Lightcycler480 (Roche) using a temperature regime consisting of 5 minutes at 95°C followed by 45 cycles at 95°C for 10 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μl of PCR-grade water (Roche), 1 μl of the corresponding primer pair (10 μM each ...
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bioRxiv - Cancer Biology 2021Quote: ... with SYBR Green PCR Master Mix (Roche) using validated primers specific to each target each gene ...
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bioRxiv - Cell Biology 2021Quote: ... on LightCycler 480 PCR System (Roche, Germany). The calculations were analyzed with 2-ΔΔCt method.
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bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed using SybrGreen (Roche) on a Roche480II Lightcycler using the following primers indicated in Table S5 ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification was performed using QuickTaq (Roche) as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2022Quote: ... and quantitative PCR (KAPA Biosystems, Wilmington, MA), according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed on LC480 (Roche) and SYBR Green Mix was from Shanghai Yeasen Biological Technology Co. ...
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bioRxiv - Cell Biology 2019Quote: ... and quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms ...
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bioRxiv - Molecular Biology 2020Quote: The Expand Long Template PCR system (Roche) with forward and reverse primers at nt 2,478–2,512 and nt 1,933–1,906 ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCRs were conducted with SybrGreen (Roche) on 384-well plates using a LightCycler 480 (Roche ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed using FastStart polymerase (Roche, 047384200010 under the following conditions ...
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bioRxiv - Genetics 2019Quote: ... A PCR reaction using KAPA polymerase (Roche), 10 ng template ...
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bioRxiv - Neuroscience 2021Quote: ... PCR-grade Proteinase K (Roche, Cat#03115828001) was added and incubated at 45°C and 650 rpm for another hour ...
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bioRxiv - Bioengineering 2022Quote: ... Quantitative PCR was performed with LightCyclerÒ480 (Roche) using TaqMan® Gene Expression Master Mix (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... with SYBR Green PCR Master Mix (Roche) using validated primers specific to each target each gene ...
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bioRxiv - Cell Biology 2022Quote: ... and LightCycler 480 quantitative PCR machine (Roche Diagnostics International AG ...
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bioRxiv - Pathology 2022Quote: ... on a PCR System (Roche, Basel, Switzerland). The PCR primers are listed in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification (Kapa Hot Start polymerase, Roche) produced a pooled library of amplicons with small overhangs homologous to a pUC19 plasmid containing the CD19 CAR HDR sequence ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... for PCRs KAPA HiFi HS RM (Roche) and different primer sets spanning regions out and/or inside expected deletions were used ...
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bioRxiv - Immunology 2023Quote: ... quantified by quantitative PCR (KK4824, KAPA Biosystems) and then sequenced on illumina NovaSeq 6000 with PE150 read length.
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bioRxiv - Cancer Biology 2023Quote: ... SYBR Green PCR master mix (Kapa Biosystems) was used for quantitative PCR in triplicate reactions with primers listed in Table S9 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expand™ Long Template PCR System (Roche) was used with the following thermocycling program ...
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bioRxiv - Neuroscience 2024Quote: ... using SYBR Green PCR Master Mix (Roche) according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems).
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Neuroscience 2020Quote: ... SYBR green quantitative PCR (qRT-PCR) was performed using the LightCycler® 480 system (Software 3.5, Roche Molecular Biochemicals). To determine the relative concentration of mRNA expression ...
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bioRxiv - Molecular Biology 2023Quote: ... V3-V4 regions of 16S rRNA genes were amplified by PCR (first stage of PCR) using 12.5µL of 2X KAPA HiFi HotStart Ready Mix (Roche, France), 5µL of amplicon PCR Forward primer 1 µM (5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG –3’) ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
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bioRxiv - Cell Biology 2019Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-DIG antibody conjugated to alkaline phosphatase allowed detection of hybridized riboprobes according to the manufacturer’s instructions (Roche).
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bioRxiv - Neuroscience 2022Quote: ... Staining of tissue and RNA detection was automated using a Discovery Ultra IHC/ISH staining platform (Roche Diagnostics). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was tested for lactate dehydrogenase using the Cytotoxicity Detection KitPLUS (LDH) (Roche, Indianapolis, IN, USA).
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bioRxiv - Immunology 2019Quote: ... RT-qPCR was performed in triplicates using the one-step system Lightcycler 480 RNA Master Hydrolysis Probes (Roche), with the following PCR profile ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR of the RT cDNA was performed according to the manufacturer’s instructions in a LightCycler 480 II (Roche Life Science using SYBR GREEN (Roche Life Science ...