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Citations for Roche :
2251 - 2300 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the dishes were placed on ice and 500 μl of homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) (4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently lysed (1 mM NaF, 1 mM Na3VO4, 10 nM calyculin A, 1 mM PMSF, 1 mg complete EDTA-free protease inhibitor cocktail [Roche] in RIPA buffer), scraped ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1% Triton X-100, 1 mM DTT, 1 mM PMSF, 1× Roche protease inhibitor cocktail) and about 500 μL of 0.5-mm-diameter glass beads ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were digested for 30 minutes and dissected adult brains for 35 minutes in proteinase K (3115836001, Roche; 10 μg/ml in PBT). To stop the reaction ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl; 1 mM EDTA; 1 mM CaCl2; 1 mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] one tablet/10 mL). The cells suspension was incubated on ice for 30 min and then centrifuged at 13,000 rpm/15 min/4°C ...
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bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR experiments were done using the HOT FIREPol® EvaGreen® qPCR Mix (08-36-00001-10, Solis Biodyne) and a LightCycler® 96 System (Roche). Expression of the 5S gene was used as a control ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
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bioRxiv - Developmental Biology 2023Quote: ... Pelleted nuclei were resuspended in 333 µl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 µl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... and the cell pellet was re-suspended in 2 ml of triturating solution (containing 10 mg/ml bovine serum albumin [A7906, Sigma], 0.5 mg/ml trypsin inhibitor [10109886001, Roche], 0.02 mg/ml deoxyribonuclease).
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bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2% Triton X-100, 1 mM EDTA, 1 mM dithiothreitol, 1 mM NaF, 10 mM β-glycerophosphate, 0.1 mM Na3VO4, 1x Roche cOmplete protease inhibitors). Protein lysates (12 µg per lane ...
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bioRxiv - Microbiology 2023Quote: Cell pellets were resuspended in 300 μL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol and Roche EDTA-free protease inhibitors). Samples were sonicated on ice using a probe-type sonicator (4 cycles of 15 s with 15 s resting on ice ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... 40 ng per sample at 10 ng/µl for cortical samples were used to perform qPCR using Light Cycler 480 Probes Master mix (Roche Diagnostics, Mannheim, Germany) on Light Cycler 480 (Roche Diagnostics ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was resuspended in 50 ml lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, Roche Complete protease inhibitor cocktail) and the lysate was boiled for 20 min ...
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bioRxiv - Physiology 2024Quote: ... 10% Glycerol, 150mM NaCl, 1mM EDTA, 1mM DTT, 2mM MgCl2, 0.5mM PMSF, 10mM NaF, 100μM PUGNAc, and Roche EDTA-free protease inhibitor cocktail) and twice with reaction buffer (20mM HEPES pH7.9 ...
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bioRxiv - Plant Biology 2024Quote: ... bacterial pellets were re-suspended in 50 ml buffer A (50 mM Tris-Hcl pH 7.5, 300 mM NaCl, 10% glycerol, 0.2% Triton X-100, 1x Roche cOmplete EDTA-free protease inhibitor), sonicated (time ...
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bioRxiv - Genomics 2024Quote: ... Quantitative PCR was performed in a 20 μl reaction mixture containing 10 μl of FastStart Essential DNA Green Master (Roche Diagnostics, Mannheim, Germany), 8μl of 2.5 ng µl-1 cDNA ...
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bioRxiv - Bioengineering 2024Quote: ... the D-PBS was removed from all the middle channel inlets and 50 µL of 28.5 µg/ml fibronectin solution (10 838 039 001; Roche Diagnostics Corp., Indianapolis, IN) in non-supplemented MCDB131 medium was added to all the top channel outlets ...
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bioRxiv - Cell Biology 2024Quote: ... antigen retrieval was performed with 20 µg/mL proteinase-K/PBS for 10 min at room temperature and DNase enzyme (Hoffmann-La Roche AG, Basel, Switzerland) was used as a positive control ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1% v/v Triton X-100, 10% v/v glycerol, 1x cOmplete protease inhibitor cocktail [Roche], and 1x PhosSTOP [Roche]). Cells were scraped off ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing HA-tagged TWIST1 interaction candidates were thawed in 300 μL hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %. DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease 0.5 μl/ ml) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Neuroscience 2021Quote: ... whole brain samples were homogenized in RIPA lysis buffer supplemented with protease inhibitors (cOmplete mini + EDTA, Roche, 1 mM PMSF, 10 μg/ml leupeptin) and phosphatase inhibitors (10 mM sodium fluoride ...
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bioRxiv - Developmental Biology 2022Quote: ... The octopus embryos were permeabilized by treating them for 15 minutes at room temperature using proteinase K (Roche, 10 μg/ml in PBS-DEPC). During the detection stage ...
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bioRxiv - Genetics 2022Quote: ... Embryos were then washed 3x 5 minutes in TBS-T (1% Tween-20 in Tris-buffered saline) and blocked for 1 hour in block solution (10% heat-inactivated sheep serum and 0.1% Roche blocking reagent in TBS-T). Roche blocking reagent was dissolved in maleic acid buffer according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: Whole-cell extracts of ∼0.5×106 cells were prepared by washing cultures in PBS and lysing with 50 μL lysis buffer (Tris pH 7.6, 10% SDS, Roche cOmplete™ Protease Inhibitor Cocktail). Western blotting was performed according to standard procedures using the following antibodies and dilutions ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in Antibody buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine, 2 mM EDTA, 0.1% BSA, and Roche complete EDTA-free protease inhibitor) with primary anti-Pol2S5p antibody (Cell Signaling Technology cat ...
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bioRxiv - Genomics 2021Quote: ... then washed and resuspended in Wash buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine and Roche complete EDTA-free protease inhibitor), aliquoted with 10% DMSO and slow-frozen to −80°C in Mr ...
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bioRxiv - Cell Biology 2020Quote: ... The buffers used in each step were supplemented with 1% (v/v) phosphatase inhibitor cocktail-2 and PhosSTOP (Roche: 1 tablet per 10 ml). Imaging of fixed samples was carried out on a Leica TCS SP8 MP microscope using oil immersion objective (HP CL APO CS2 63x/1.40 Oil) ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein extraction cells were resuspended in protein extraction buffer (1xPBS, 10% glycerol, 1mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail), and lysed in a bead beater ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions (10 µl) were set up in Light cycler 480 plates using the LightCycler 480 probe master mix (Roche, Welwyn Garden City UK) according to the manufacturer’s instructions (Tables 2) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 50 mL lysis buffer (20 mM Tris pH7.5, 200 mM NaCl, 2 mM β-mercaptoethanol, 10% glycerol, protease inhibitor cocktail (Roche, as directed by manufacturer), 20 mM imidazole) ...
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bioRxiv - Physiology 2022Quote: ... The first PCR reactions were carried out in a total volume of 10 μL that consisted of 5.0 μL KAPA HiFi HotStart ReadyMix (KK2602; KAPA Biosystems, Potters Bar, Herts., UK.), 0.25 μL each primer (10 pmol/L) ...
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bioRxiv - Molecular Biology 2022Quote: ... Frozen cell pellets were re-suspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 10% glycerol, pH 8) containing protease inhibitors (Roche, complete mini, EDTA-free). Following lysis by sonication and centrifugation at 20,000 r.p.m ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of nuclear extract of KDM3A and KDM3B endogenously tagged ESCs (prepared as above) was incubated with 10 µg of HA antibody (Roche, clone 12ca5, catalog#11583816001) for 4 hours rotating at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM KAc, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, 0.1 mM ATP, 20 mM PMSF, and 10 Roche protease inhibitor tablets per L) and lysed by a dounce homogenizer ...
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bioRxiv - Neuroscience 2023Quote: ... approximately 40 ng of the first strand cDNA was amplified in 10 μl of reaction mixture using the LightCycler 480 SYBR Green I Master reaction mix (Roche Applied Science, Mannheim, Germany) and a LightCycler 480 Instrument (Roche Applied Science ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM NaCl, 0.1 mM EDTA, 10% [v/v] glycerol, 1% [w/v] digitonin, 2 mM PMSF, 1 x Roche EDTA free protease inhibitor cocktail). After removing the debris by a clarifying spin (12,000 x g ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in non-denaturing buffer at 4°C (10 mM Tris, 500 mM NaCl, MgCl2 1mM, 3U of DNase I, and protease inhibitor cocktail 1 X from Roche, ref 4693116001, pH 7.9) followed by sonication and ultracentrifugation at 13000 rpm for 45 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 10 minutes at 37°C in a 3% (w/v) collagenase A solution in PBS (Collagenase A, Roche Diagnostics GmbH, Germany, 10103586001). Cells were recovered by centrifugation for 5 minutes at 300g prior cell counting on Malassez cells after Trypan blue staining ...
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bioRxiv - Cell Biology 2023Quote: ... and lysis buffer A (50 mM Tris-HCl, 5 mM EDTA, 10 mM NaN3, and Complete™ EDTA-free tablet; Roche Diagnostics, Mannheim, Germany); disrupted with glass beads at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... in presence of MBP (10 μg) in the kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... were cultured in RPMI 1640 supplemented with 20% FBS and 10 U/mL recombinant human interleukin-2 (hIL-2; Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Developmental Biology 2024Quote: ... L2 and L3 larvae were collected in a Tris-lysis buffer (10 mM Tris, 150 mM NaCl, 1% Triton X-100, 25 mM EDTA added of EDTA-free Roche complete protease inhibitor cocktail) and homogenized to extract soluble proteins ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was carried out using 2 µl of 10-fold diluted cDNA in a final volume of 10 µl with the LightCycler 480 SYBR Green I Master 2X (Roche Diagnostics GmbH, Mannheim, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...