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Citations for Roche :
2101 - 2150 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8) supplemented with Proteinase Inhibitor Cocktail (Roche, 11697498001) and chromatin was sonicated using the Bioruptor pico (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM TCEP and 10 % v/v glycerol) enriched with 4x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001). Cells were lysed by sonication using a 10 mm tip on a VC 750 ultrasonic processor (Sonics) ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei from 600,000 cells were isolated by hypotonic lysis in 1 mL buffer NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 0.5 mM spermidine; 0.1% Triton X-100; 20% Glycerol; Roche EDTA-free protease inhibitor) for 10 minutes on ice followed by centrifugation at 1300 xg for 4 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then discarded and the pellet was resuspended in 300 µL SDS buffer (4 % SDS, 10 mM EDTA, 25 mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) and incubated at room temp (10 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... The tissue was then washed with PBT extensively (10 times or more for 5 minutes) before development with BM-purple (1ml per well, Roche Diagnostics). Time of development was approximately 20 minutes at room temperature to 24 hours at 4°C depending on the probe ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified using 22 cycles of PCR amplification from approximately 300 µg genomic DNA (10 µg DNA/100 µL reaction) using 2X KAPA HiFi HotStart ReadyMix (Roche KK2602), a combination of 8 staggered P5 primers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were maintained in growing media (DMEM + Glutamax, 10% FBS, pen/strep 5ml) supplemented with 0.8mg/ml G418 (Roche, G418-RO) for maintenance or removed for exposure to chemicals ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Immunology 2023Quote: ... then subject to gentle cold digestion on ice using 10% FBS-supplemented RPMI media containing 100mg/mL trypsin inhibitor from soybean (Roche, 10109886001), 10mg/mL collagenase A from clostridium histolyticum (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Physiology 2023Quote: ... The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... Crypts were isolated from the distal half of the small intestine as described above and dissociated in 10% FBS in 1X PBS supplemented with 20 μg/mL Liberase TH (Roche 05401135001), and 35 μg/ml DNaseI (Roche 10104159001 ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Developmental Biology 2024Quote: ... Cryosections were then washed 3 times for 5 minutes in PBT before being incubated with 1:10 TUNEL enzyme buffer (Roche #12156792910) at 37°C in the dark for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... This was done by using cDNA from 10 ng total RNA with 2x FastStart Universal Probe Master ROX (Roche, Basel, CHE) and the corresponding TaqMan Assay in a StepOnePlus Real-Time PCR Cycler (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Physiology 2024Quote: ... TA muscles were dissociated and digested in DMEM F/12 medium containing 10 mg/ml of collagenase B and 2.4 U/ml Dispase (Roche Diagnostics GmBH) at 37°C for 30 min and passed through a 30 μm cell strainer ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was carried out in a 10 µL reaction volume using a LightCycler® 480 SYBRTM Green I Master (Roche) kit ...
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bioRxiv - Neuroscience 2024Quote: ... or 25% of the RT reaction) was amplified using 10-12 cycles of PCR with KAPA HotStart PCR ReadyMix (Roche KK2601) and custom sample index primers (P7 primers ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 0.1% NP-40, 0.5 mM DTT, 1 mM PMSF, and 1% Roche protease inhibitor cocktail) and rotated for 30 min at 4℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were ground in liquid nitrogen and homogenized in 40 ml of NEB1 buffer (10 mM Tris-HCl [pH 8.0], 0.4 M sucrose, 10 mM MgCl2, 5 mM DTT, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet). The homogenate was filtrated through two layers of Miracloth ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were blocked in 10% sheep serum and incubated overnight at 370C with anti-DIG-AP antibody (Roche at 1:1000). Signal detection was done using NBT/BCIP substrate (Roche).
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in ice cold radioimmunoprecipitation assay (RIPA) buffer containing 1:10 PhosSTOP™ (#4906845001, Roche Diagnostics, Indianapolis, IN, USA) and 1:20 cOmplete™ ULTRA Protease Inhibitor Cocktail (#06538304001 ...
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bioRxiv - Microbiology 2024Quote: ... Then tissue was rinsed three times in ice-cold RPMI-1640 and digested in 5 ml of complete media (RPMI-1640 containing 10% FBS, 1% antibiotic/antimycotic, 5mg/ml DNase I) supplemented with 5 mg/mL Liberase™ (Roche) and 5mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM imidazole) supplemented with DNase and one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche #11 873 580 001). Cells were lysed using a French press (2x ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Cells were grown to ∼80% confluency, washed in PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, and protease inhibitors (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed in 384-well plates with a total volume of 10 μL and the reaction mixtures were run on a LightCycler 480 system (Roche, Germany). Data are shown as the relative mRNA expression compared to 0% MgCl2 test with the endogenous reference 16S rRNA gene.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were extracted using extraction buffer (50 mM Tris-HCl pH 7.5, 20 mM NaCl, 10% glycerol, 1% Triton, and a 1:7 ratio of protease inhibitor; 04693124001, Roche, Basel, Switzerland), based on a previously described extraction buffer (55) ...
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bioRxiv - Physiology 2024Quote: ... animals were anesthetized and transferred to 96-well PCR plates (USA Scientific 1402-9598) in a homogenization buffer (10% sucrose/50 mM EGTA/1X cOmplete EDTA-free protease inhibitor cocktail tablet (Roche 11873580001)) ...
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bioRxiv - Genomics 2024Quote: ... 10-30ng of the immunoprecipitated material was used for ChIP-seq library preparation using the KAPA Hyper prep kit (KAPA Biosystems) and applying 7-8 cycles of amplification ...
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bioRxiv - Plant Biology 2024Quote: ... The seedlings were then ground into a fine power in liquid nitrogen and resuspended in buffer I (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.4 M sucrose, 1 mM DTT, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet) for 45 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The PCR cycle was determined for each library using 1/10 of the adaptor-ligated DNA as the template with the KAPA SYBR FAST qPCR Master Mix (Roche, KK4603) by taking the Ct that reaches 1/2 of the maximum intensity and subtracting 2 cycles (Ct -2 cycles) ...
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bioRxiv - Cell Biology 2024Quote: Unsynchronized population of worms or L4 larvae after aHS+3h were harvested in M9 from OP50 plates and the pelleted worms were lysed in equal volume of PBS Triton 2% + 10% PIC (Protease inhibitor cocktail, Roche 1183617), and glass beads (425-600µm ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissues were then cut into small pieces using sharp dissection scissors and incubated in 10 mL dissociation solution (0.2 mg/mL Collagenase D (Roche, Cat#11088866001); 0.05 mg/mL DNase I (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... and total of 10 ng DNA was analyzed by quantitative PCR using FastStart Universal SYBR Green master mix (Roche, Basel, Switzerland) with primers directed against gDNA or mtDNA ...
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bioRxiv - Genetics 2024Quote: ... The IPC qPCR assay was performed in duplicate using 10 μl of 2x SYBR Green I Master (Roche Diagnostics, Mannheim, Germany), 0.2 μl each MSR_129 primer ...
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bioRxiv - Developmental Biology 2024Quote: ... 67°C for 20 secs and 72°C for 10 mins) in a thermal cycler with KAPA Hifi HotStart ReadyMix (Roche, #9420398001). Pre-amplication cDNA mix was purified with 0.8X SPRI beads and quality of cDNA was assessed with a D5000 Screen Tape assay (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... and used in a 10 μL RT-qPCR reaction composed of 5 μL 2x KAPA SYBR FAST qPCR Master Mix (Roche, KK4600), 2.5 μL cDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were instead smashed by pipetting up and down with 90 µl ice-cold PBSt and 10 µl Protease Inhibitor (Roche, #04693116001) and the samples were centrifuged at 4 °C at 14400 g for 10 minutes.
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bioRxiv - Cell Biology 2024Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR reactions were 10 µL and contained 1x KAPA Sybr Fast Low-Rox qPCR Master Mix (KK4621; Roche Sequencing, Pleasanton, CA, USA) with 200 nM each primer ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were made from 1∼2 mL packed worms in lysis buffer (25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 10% glycerol, 1x cocktail of protease inhibitors from Roche (Complete, EDTA free), 40 mM NaF ...
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bioRxiv - Genetics 2020Quote: ... TBS (Tris-buffered saline: 10 mM Tris-HCl, pH 7.4, 150 mM NaCl) containing 1X Western Blocking Reagent (Roche Applied Science, Inc.). Antibodies were diluted in TBS containing 0.5X Western Blocking Reagent + 0.1%Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were dehydrated and rehydrated in ethanol and water and incubated with 10 μM dNTP mix containing Digoxigenin-dUTP and 20 U/mL Klenow Fragment (Roche; Basel, Switzerland) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed on Roche LightCycler® 96 in 10-μl reactions using FastStart Essential DNA Green Master (Roche, Cat. No. 06402712001). A housekeeping gene Rplp0 was used as an endogenous reference gene ...
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bioRxiv - Genomics 2022Quote: ... then incubated with primary antibody at 4°C overnight in Wash Buffer (10 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) with 2mM EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed with an IP lysis buffer (150 mM NaCl, 25 mM Tris-HCl, 10% glycerol, and 1 mM EDTA) containing a protease inhibitor cocktail (Roche, Basel, Switzerland). IP was performed with an anti-Prdx5 antibody (Ab Frontier) ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse MEC organoids were prepared by manual chopping of mouse mammary gland tissues harvested from 10–12-week-old mice followed by digestion with shaking for 1 hr at 37 °C with Collagenase A from Clostridium histolyticum (10 mg/mL; Roche, Cat. #: 13560925) and Hyaluronidase from bovine testes (1 mg/mL ...