Labshake search
Citations for Roche :
2151 - 2200 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared by scraping cells using 1X lysis buffer (10X recipe-50 mM Tris pH 7.5, Triton 20%, NP40 10%, 2 M NaCl, mixed with cOmplete protease inhibitor tablet - Roche, Product number 11873580001). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 13,000 rpm 4°C to pellet the cell debris ...
-
bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
-
bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... containing Roche mini-complete protease inhibitor EDTA-free and Roche PhosSTOP (1 tablet of each per 10 mL of buffer) (Roche, Basel, Switzerland). Samples (on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% ADC enrichment (Middlebrook Albumin Dextrose Catalase Supplement, Becton Dickinson, Oxford, UK) and hygromycin (50 mg/l; Roche, Lewes, UK), the culture was washed twice with sterile PBS ...
-
bioRxiv - Microbiology 2021Quote: Bacteria-captured beads were incubated at 37°C for 1 hour with 10 mg/mL of lysozyme (Cat # 10837059001, Roche, Basel, Switzerland) diluted in 10 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were lysed in Tris-lysis buffer (10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1% Triton X-100, 10% glycerol, and cOmplete™ protease inhibitor cocktail [Roche, Basel, Switzerland]) on ice ...
-
bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
-
bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in Buffer 2 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 10 min followed by a centrifugation step ...
-
bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
-
bioRxiv - Immunology 2020Quote: ... Membranes were blocked for 1 h with 3% (w/v) non-fat milk powder in PBS/0.1% (v/v) Tween (PBST) (CALR, 10% Roche WBR in PBST) and incubated with primary antibody (Supplementary Table III ...
-
bioRxiv - Pathology 2020Quote: ... Microwave decloaking was performed in 10 mM sodium citrate (pH 6.0) and non-specific sites were blocked in TBSTw containing 1% Blocking Reagent (Roche Diagnostics, Indianapolis, IN), 5% normal goat or donkey sera ...
-
bioRxiv - Immunology 2021Quote: Adipose and other tissues were removed from isolated thymi which were subsequently subjected to three rounds of enzymatic digestion for which cut up lobes were incubation at 37°C in Liberase digestion buffer (PBS Gibco 1X, Liberase Roche 2.5 mg/ml, DNase Roche 10 mg/ml). Cells were incubated with anti-CD45 beads (Miltenyi Biotec ...
-
bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
-
bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
-
bioRxiv - Physiology 2021Quote: Pancreases were rapidly dissected after intra-ductal injection from 9-10 week-old male mice and islets were isolated by digesting the pancreas with collagenase P (Roche, Basel, Switzerland) and purified using a Ficoll gradient (Merck Biochrom ...
-
bioRxiv - Immunology 2020Quote: ... The cDNA samples were diluted 1:10 in water and analyzed in triplicate using a LightCycler 480 detection system (Roche, Meylan, France). PCR conditions were 95°C for 5 min and 45 cycles of 95°C for 15 s ...
-
bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 0.05% NP-40, 10% glycerol, 1×Roche protease inhibitor cocktail). Cell lysates were prepared by bead-beating ...
-
Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... followed by addition of 100 μL ChIP Elution Buffer (50 mM Tris-HCl, pH 7.5 @ RT, 10 mM EDTA, 1% SDS) and 20 μL Pronase (Roche, 20 mg/mL) for incubation at 42°C for 2 hours ...
-
bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, 10% glycerol, 1% IGEPAL [Sigma, St Louis, MO], and cOmplete EDTA-free protease inhibitor cocktail [Roche] [pH 7.4]). Cells were vortexed ...
-
bioRxiv - Genomics 2023Quote: ... buffer (HEPES-KOH pH-8.2 [20 mM], KCl [10 mM], Spermidine [0.5 mM], IGEPAL [0.05%], Glycerol [20%], Roche Complete Protease Inhibitor EDTA-Free). The nuclei were resuspended after the final wash in 40 μl NE per sample and bound to 10 μl Magnetic ConA Agarose beads equilibrated in binding buffer (HEPES pH 7.5 [20 mM] ...
-
bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... buffer (HEPES-KOH pH-8.2 [20 mM], KCl [10 mM], Spermidine [0.5 mM], IGEPAL [0.05%], Glycerol [20%], Roche Complete Protease Inhibitor EDTA-Free). After extraction ...
-
bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... Culture pellets were sonicated in 50 ml Lysis Buffer (10 mM Tris-HCl pH 7.9, 300 mM KCl, protease inhibitors from Roche according to manufacturer), spun at 18K rpm ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 1 mM PMSF, 3 mM ATP, 10% sucrose and Roche protease inhibitors). For sS1 constructs ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 10% sucrose, 3 mM ATP, 1 mM DTT, 1 mM PMSF and Roche Protease Inhibitors), 1 ml per dish ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
-
bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, 0.05% NP-40, 10% glycerol, 1×Roche protease inhibitor cocktail) and were lysed by beating with 0.5-mm-diameter glass beads using a FastPrep instrument at a speed of 6.5 m/s for three cycles of 20 seconds each ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were first incubated 1 hour with Blocking Buffer (10% heat-inactivated Goat Serum, 0.5% Blocking Reagent [Roche] in PBS-0.5% Tween) and then 1 hour with 1.5U/ml αFAM-POD antibody (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl, 20 mM Tris-HCl pH 8, 2.5 mM EDTA, 10 % glycerol, 1 % Triton, 1x of PhosSTOP, 1x Roche protease inhibitor mixture) and given 20 strokes with the Dounce Homogenizer ...
-
bioRxiv - Microbiology 2023Quote: ... Crude extract was obtained from a 500ml culture bacterial pellet by successive sonications in 2×20ml buffer A (30mM Tris pH8.0, 10% Glycerol, 1M NaCl, 10mM Imidazole pH8.0, 1x Protease Inhibitors Cocktail (Roche Complete Mini EDTA free), 0.4mM PMSF ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Cell pellets were lysed in 300 µL of lysis buffer (10 mL BugBuster® [Millipore 70584-4], ¼ tablet cOmplete™ Protease Inhibitor Cocktail EDTA-Free [Roche]) for 20 min at room temperature and centrifuged at 13,000 rpm at 4℃ for 30 min to clear all cell debris ...
-
bioRxiv - Neuroscience 2023Quote: ... and the striatal slices were incubated at 30°C in Krebs-HCO3-buffer (Mg2+ free) with 10 μg/mL adenosine deaminase (Roche, Basal, Switzerland) for 30 min under constant oxygenation with 95% O2/5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8 and 10 mM NaF completed with 1 × protease inhibitor cocktail (Pierce, #A32955) and 1 × phosphatase inhibitor cocktail (Roche PhosSTOP, #490684001). The total protein concentration was determined using Bradford assay (Protein Assay Reagent ...
-
bioRxiv - Cell Biology 2023Quote: ... Synthesized oligonucleotides (Twist Biosciences, South San Francisco, CA) were PCR-amplified for 10 cycles using KAPA HiFi HotStart DNA Polymerase (Roche, Basel, Switzerland) with 52 °C annealing and 15 s extension ...
-
bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free, Roche, Laval, Canada). The samples containing lysis buffer were then collected and spun down for 5 minutes in pellet the cell debris ...
-
bioRxiv - Developmental Biology 2022Quote: ... buffer (HEPES-KOH pH-8.2 [20 mM], KCl [10 mM], Spermidine [0.5 mM], IGEPAL [0.05%], Glycerol [20%], Roche Complete Protease Inhibitor EDTA-Free), resuspended in 40 μl NE per sample and bound to 20 μl Magnetic ConA Agarose beads equilibrated in binding buffer ...
-
bioRxiv - Genomics 2022Quote: ... buffer (HEPES-KOH pH-8.2 [20 mM], KCl [10 mM], Spermidine [0.5 mM], IGEPAL [0.05%], Glycerol [20%], Roche Complete Protease Inhibitor EDTA-Free), then resuspended in 40 μl NE per sample and bound to 20 μl Magnetic ConA Agarose beads equilibrated in binding buffer ...
-
bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed in 4 replicates in final volume of 10 µL using Light SYBR Green PCR Master Mix (Roche, Applied Science) on a LightCycler 480 Real-Time PCR System (Roche ...
-
bioRxiv - Immunology 2022Quote: ... colon tissue was homogenised in 350 µl of lysis buffer (40 ml of PBS, 10%FCS, 2 Complete Protease Cocktail Inhibitor Tablets (Roche, Mannheim, Germany). Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl2, 1% Triton X-100, 10% Glycerol, 1 mM DTT, Roche complete protease inhibitor) with 0.5 mg/mL Lysozyme and placed on a roller for 45 minutes at 4 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on cDNAs in a final volume of 10 µL using SYBR Green I master mix (Roche Life Science) and primers for AtCLCa gene ...
-
bioRxiv - Neuroscience 2022Quote: Cells were homogenized in 1 % NP40 lysis buffer (20 mM HEPES pH7.5, 150 mM NaCl, 1 % NP-40, 10 % Glycerol and Roche protease inhibitor mix) on ice ...
-
bioRxiv - Immunology 2022Quote: ... cells were collected 24 h after incubation with 40 ng/ml IL-10 following the protocol of the High Pure RNA Isolation Kit (Roche, Basel, Switzerland).
-
bioRxiv - Bioengineering 2023Quote: ... 10-12 DRGs per donor were disintegrated into micro tissue units using 6 mL collagenase P (4 mg/mL, Roche, Mannheim, DE) in a 15 mL Falcon tube on an orbital shaker for 2 h at 37 °C ...