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Citations for Roche :
1851 - 1900 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Microbiology 2020Quote: Cells were washed once with ice-cold PBS and lysates harvested at the indicated times post infection with lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – complete mini EDTA-free protease inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the pellet was resuspended in 45 µl 10 mM sodium phosphate buffer supplemented with EDTA-free Protease Inhibitor Cocktail (Roche) and incubated (5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in nuclear extraction buffer (20mM HEPES pH7.5, 10% glycerol, 350mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) and incubated for 30 min at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... The crude nuclear pellet was rinsed in wash solution (20mM HEPES pH7.5, 10% glycerol, 150mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) twice ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mM Tris-HCl (pH 7.4) and 1 mg/ml BSA) supplemented with 10 μl of DIG RNA labeling mix (Roche 12039672910) and incubated for 1 h at 37°C with occasional gentle tapping ...
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bioRxiv - Neuroscience 2021Quote: ... in sucrose lysis buffer (270 nM sucrose, 10 mM Tris-HCl, 1 mM NaHCO3, 1 mM MgCl2, protease inhibitor (cOmplete Mini, Roche), phosphatase inhibitor (PhosphoStop ...
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bioRxiv - Neuroscience 2021Quote: ... there is a need to stop it after 2 hours by a single injection of diazepam (Valium®, 10 mg×kg−1, i.p.; Roche). Rats were then hydrated with 2 mL of saline solution (0.9% NaCl ...
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bioRxiv - Pathology 2022Quote: ... 2 μl of cDNA in RNase free water was made up to 20 μl with FastStart Universal SYBR Green Master (ROX, 10 μl, Roche), Ultra Pure Water (6.4 μl ...
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bioRxiv - Genomics 2022Quote: Human embryonic hindlimb muscles were collected in sterile PBS and mechanically dissociated using fine scissors in 10 mL (per gram of tissue) of enzyme solution containing 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
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bioRxiv - Genomics 2022Quote: ... Tissue was then mechanically dissociated with fine scissors in 10 mL (per gram of tissue) enzyme solution mixed with 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
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bioRxiv - Cell Biology 2022Quote: 2 μL of FLAG or IgG pull down and of a 1:10 dilution of the INPUT were used for amplification using qPCRBIO SyGreen Mix (PCR Biosystem) in a LightCycler96 (Roche) instrument ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: A unique Fluidigm barcode was added to each sample by PCR in 10 μl reactions using the Fast Start High Fidelity PCR System (Roche) containing ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were harvested in ice-cold PBS using a cell scraper and lysed by sonication for three times 10 seconds in ice-cold PBS containing Complete protease inhibitors (Roche). Unbroken cells were removed by centrifugation at 500 g for 2 minutes ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (10 mg/kg for the first dose with each subsequent dose at 5 mg/kg; atezolizumab, Roche), anti-TGF-β (10 mg/kg ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were washed in MABT buffer for one hour and NTMT buffer for 10 min and incubated in NBT/BCIP (Roche) for the coloring reaction ...
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bioRxiv - Cell Biology 2020Quote: Trichonympha and Teranympha cells were extracted from the hindgut of termites in 10 mM K-PIPES in the presence of cOmplete protease inhibitor cocktail (1:1000; Roche) as described (Guichard et al ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2, 10% glycerol, 1% Tween-20, 20 mM imidazole, 1 mg/ml lysozyme, and 1x protease tablet [Roche]) for thirty minutes on ice with 5 min intervals of 10 s vortexing ...
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bioRxiv - Plant Biology 2020Quote: ... in a reaction volume of 20 μL containing 10 μL of the LightCycler 480 SYBR Green I Master Mix (Roche), 0.3 μM of each specific forward and reverse primer (Table S9 ...
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bioRxiv - Microbiology 2020Quote: After adding 1 mL of PBS (pH 7.4) containing protease inhibitor (1 tablet per 10 mL, Roche cOmplete™, #04693159001) and 500 μL of 0.5 mm diameter glass beads ...
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bioRxiv - Microbiology 2021Quote: ... The cell pellet was resuspended in 10 ml PdeL purification buffer and one tablet of c0mplete mini EDTA-free protease inhibitor (Roche) and a spatula tip of DNase I (AppliChem ...
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bioRxiv - Developmental Biology 2020Quote: ... and blocked in 10% Sheep Serum for 2 hours followed by incubation with an anti-DIG antibody (1:2000) (Roche) in TBST / 1% sheep serum overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 % sodium deoxycholate and 1 % Triton X-100 as well as protease inhibitors (10 mg / ml leupeptin, pepstatin A, 4-(2-aminoethyl) benzensulfonyl-fluorid and aprotinin) and phosphatase inhibitors (PhosSTOP−, Roche). Total cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Genomics 2021Quote: ... each cell pellet was resuspended in 500 uL of Nuclear Extraction Buffer (NEB, 20 mM HEPES pH 7.9, 10 mM KCl, 0.5mM Spermidine, 0.1% NP40, 20% glycerol, Roche Proteinase Inhibitor tablets) by pipetting gently ...
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bioRxiv - Cancer Biology 2021Quote: ... individual primary tumors were dissected from the lungs of KL mice, mechanically dissociated, then digested for 45min in digestion media (10% FBS, pen/strep, 1mg/mLCollagenase/Dispase (Roche) in DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Biochemistry 2021Quote: Cell extracts were prepared in 1% NP40 lysis buffer (20 mM Hepes pH 7.5, 150 mM NaCl, 1% NP40, 50 mM NaF, 1 mM Na3VO4, 10% glycerol, and protease inhibitor cocktail from Roche) at 4 °C for 15 mins ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was homogenized by probe sonication at 20kHz for 10s in ice-cold TBS buffer (tris-buffered saline, 1% Triton X-100; pH 7.4) containing protease inhibitor (Roche 11697498001) and phosphatase inhibitor (Sigma 4906845001 ...
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bioRxiv - Neuroscience 2021Quote: ... MGEs pooled from a single litter were incubated with 200 µL papain solution supplemented with 10 U/mL recombinant DNase I (Roche) for 10 minutes at 37 °C on a rocking platform ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were pelleted by spinning at 500 g for 5 min at 4°C and resuspended in 1 mL cold CUT&RUN wash buffer (20 mM HEPES pH 7.5, 0.2% Tween-20, 150 mM NaCl, 0.1% BSA, 0.5 mM spermidine, 10 mM sodium butyrate, Roche Protease Inhibitor Cocktail). 10 μL concanavalin-coated bead slurry (Bangs Laboratories (Fishers ...
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bioRxiv - Plant Biology 2021Quote: ... SYBR Green I master mix was used for quantitative real-time PCR (qRT-PCR) in a total reaction volume of 10 μland analyzed by LightCycler 480 instrument (Roche) as described by Koskela et al. ...
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bioRxiv - Genetics 2021Quote: ... The cultures were spun down and cell pellets were resuspended in 500 μL of Workman Extract Buffer (40mM HEPEs pH7.4, 250mM NaCl, 0.1% NP40, 10% Glycerol, 1 mM PMSF, Roche proteinase inhibitors). 250 μL of glass beads were added and cells were lysed with Peqlab precellys homogenizator (3×30 sec) ...
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bioRxiv - Immunology 2020Quote: ... Bacterial pellets obtained by centrifugation at 13,500g for 10 min at 4°C were resuspended in 1 ml of PBS containing protease inhibitors (cOmplete Mini, Roche; 1 tablet per 10 ml of PBS following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the bacterial cells were suspended in 20 ml cold buffer A (500mM NaCl, 50mM Tris pH 7.5, 5% glycerol, 10 mM imidazole) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 100 ug/ml lysozyme ...
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bioRxiv - Neuroscience 2020Quote: ... digested using 0.1 % papain at 37°C for 10 min and for 5 additional minutes in presence of DNAse1 (1 mg/ml, Roche). After stopping papain activity by adding MC+ media ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... gel pieces briefly rinsed with 50 mM AmBic and rehydrated in a small volume (10 μL) of 50 mM AmBic supplemented with 1 U PNGase F (Roche) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed once with PBS and resuspended in ice-cold WCE buffer (10 mM Tris-HCl, 1% NP-40, 2 mM MgCl2, benzonase, cOmplete Protease Inhibitor Cocktail (Roche), 25 nM NEM where relevant ...
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bioRxiv - Biophysics 2021Quote: ... 10% w/v glycerol, 20 mM imidazole, 5 mM 2-mercaptoethanol, 1 mM PMSF, 3 U/mL benzonase, 1X Roche complete protease inhibitor without EDTA) ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM NaCl, 50 mM sodium pyrophosphate, 50 mM sodium fluoride, 1% Triton-X-100, 10% glycerol, 5 mM EDTA, Roche MiniProtease Inhibitor cocktail tablet ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenized in a ‘homogenization buffer’ (Sucrose, 100 mM EGTA, 10 mM Tris, protease and phosphatase inhibitors (Roche), pH 8.1 ...
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bioRxiv - Cancer Biology 2022Quote: Frozen bacteria pellets were thawed on ice and loosened in 25 mL lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X100, 10 mM DTT, 1X Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with 10 ml of cold 1x PBS supplemented with proteinase inhibitors and phosphatase inhibitors (Roche # 4693132001) and the pellets were snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... cells were harvested then resuspended in lysis buffer (25 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol) supplemented with cOmplete™ EDTA- free protease inhibitors (Roche). Cells were lysed using a Dounce homogeniser followed by sonication on ice (60% amplitude ...