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Citations for Roche :
1951 - 2000 of 9230 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication in 50 mL lysis buffer (20 mM Tris pH 8.5, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 2x Roche protease inhibitor tablets ...
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bioRxiv - Microbiology 2020Quote: ... DNA was first eluted from the column in 100 µL DNA elution buffer and subsequently the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and finally RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue samples were freshly isolated and lysed in 200 μ l of the extraction buffer (100 mM Tris-pH7.5, 125 mM NaCl, 0.1 mM EGTA, 0.1% TritonX-100, Roche® protease inhibitors cocktail), sonicated ...
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bioRxiv - Neuroscience 2022Quote: ... tissue sections were rinsed three times within 24 h with PBS plus Triton X-100 (PBST; 0.1% (vol/vol) Triton X-100 (Roche, Mannheim, Germany)) under gentle agitation at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were lysed with 5 mL nuclear lysis buffer (60 mM HEPES pH 7.5, 10 mM MgCl2, 100 mM KCl, 0.1% Triton X-100, 10% glycerol, Roche cOmplete protease inhibitor) and sonicated for 8 cycles with 10 s on and 50 s off16 ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.2 mg/mL collagenase with 60 U/mL DNase I (Roche). At the end of each round of digestion ...
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bioRxiv - Immunology 2020Quote: ... and digested in 0.34 U/ml Liberase TM (0.06mg/ml, Roche) as previously described for 60 minutes at 37°C with manual agitation every 15 minutes (Zychlinsky Scharff et al. ...
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bioRxiv - Immunology 2020Quote: ... 10μg/ml DNase I and 1.5mg/ml dispase II (all Roche). The LP lymphocyte-enriched population was harvested from a 40%-80% Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µg/ml streptomycin and 0.4 mg/ml Liberase™ (Roche). The tissue digest was stopped by addition of an equal volume of RPMI supplemented with 10% heat- inactivated FCS ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Microbiology 2022Quote: ... We excluded those with evidence of previous COVID-19 (defined as a positive NAAT COVID-19 test or presence of anti-nucleocapsid antibodies in the blood [Roche, IND, USA] 7) given the known differential impact on antibody responses post-vaccination.8 We also excluded cases for whom the second vaccine was >130 days after the first ...
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bioRxiv - Paleontology 2022Quote: ... The final libraries were quantified on an Applied Biosystems™ QuantStudio™ 7 Flex Real-Time PCR System using the KAPA SYBR® FAST ROX Low qPCR Master Mix for Illumina platforms (KAPA Biosystems, KK4873). DNA extracted from mammoth and sloth boluses were sequenced on an Illumina MiSeq using the 600-cycle v3 kit (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) were used for the colorimetric detection of alkaline phosphatase activity ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Blocking was then performed with DBPS containing 3% BSA (Roche, Cat#3116956001) and 1% Chicken serum (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5-bromo-4-chloro-3’-indolyphosphate (NBT/BCIP) and FastRed (Roche, Germany) staining was performed according to established published protocols62,63 using the following DIG and FITC labeled probes:
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bioRxiv - Molecular Biology 2023Quote: ... and 3 µl 10 mM dNTP (KAPA HiFi Kits from Roche, KK2502) were added to the annealed oligos ...
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bioRxiv - Developmental Biology 2024Quote: ... Beads were washed 3 times with lysis buffer containing proteinase inhibitors (Roche) and 7 times with lysis buffer without proteinase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Cultured cells were harvested at different time points as indicated in the figures and lysed in 1% Triton X-100 buffer containing protease inhibitor cocktail (Roche diagnostics, # 11-836-170-001), 1 mM PMSF ...
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bioRxiv - Immunology 2022Quote: ... The tonsil and lung were gentleMACS dissociated in 5mL digestion buffer (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and then agitated on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... were placed in 5mL of digest media (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and homogenized in gentleMACS C tubes (Miltenyi Cat#130096334 ...
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bioRxiv - Cell Biology 2023Quote: ... 4 ml of HBSS (without Ca2+ or Mg+) and 10 ml of collagenase solution [2 mg/ml collagenase H (11074059001; Roche), 0.1 mg/ml DNaseI (10104159001 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2 % Triton X-100) with protease inhibitors (2x Roche Complete EDTAfree ...
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bioRxiv - Immunology 2021Quote: ... 0.25% Triton X-100) with protease inhibitors (Roche 11873580001) and Na3VO4 (180µg/ml ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 0.25% Triton X-100 and protease inhibitor cocktail (Roche)] on ice for 5 min and nuclei released with 10 strokes in a Dounce homogeniser ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Triton X-100 and protease inhibitor cocktail (Roche). Lysates were cleared by centrifugation and protein concentrations determined using a BCA assay (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... and detergent extracted in 0.5% Triton X-100 (Roche) for 3min.
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bioRxiv - Genomics 2022Quote: ... 0.25% Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... containing 0.15% Triton X-100 and protease inhibitors (Roche). Following centrifugation at 16,000 gav for 20 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 100 mM NaCl [containing protease inhibitor cocktail (Roche)] ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5% Triton X-100 and stained with BMPurple (Roche) at room temperature until the signal was optimal ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were permeabilized with 0.025% Triton-X-100 (Roche) in 1X TBS (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 μL 50% anti-HA affinity matrix (Roche 11815016001) was added to the lysate and incubated 12 h at 4°C in experiment 1 and 60 μL of 50% anti-HA-agarose (Sigma A2095 ...
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bioRxiv - Neuroscience 2022Quote: ... Signal development was performed with 100% BM-Purple (Roche) in the dark (Fig ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100 and protease inhibitor tablets (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Triton X-100 and protease inhibitor cocktail (Roche), pH 7.5.
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bioRxiv - Cancer Biology 2023Quote: ... 0.25% Triton X-100) supplemented with protease inhibitors (Roche) (1 mL per 15 cm dish) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Triton X-100 and protease inhibitor cocktail (Roche), pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl) supplemented with cOmplete protease inhibitors (Roche) for 5 min on ice ...