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Citations for Roche :
2201 - 2250 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... based on the 5′ and 3′ RACE kit (2nd generation, Roche, Basel, Switzerland). Viral nucleic acids were extracted from 140 μl of the virus transport medium derived from the nasopharyngeal swab using the viral RNA isolation kit according to the manufacturer’s protocol (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by digesting the RNA with 3 µg RNase A (Roche) and 30U RNase T1 (Roche ...
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bioRxiv - Immunology 2022Quote: ... Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche) at 6,000 rpm for 15 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche) and dGTPs ...
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Developmental Biology 2023Quote: ... After a brief enzymatic treatment with a cocktail of Liberase Blendzyme 3 (Roche), trypsin B (BI) ...
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bioRxiv - Immunology 2021Quote: ... 10 μg/ml DNase I and 1.5 mg/ml dispase II (all Roche). The LP lymphocyte-enriched population was harvested from a 40%-80% Percoll (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... transferrin (5 μg/mL) and sodium selenite (5 ng/mL) (Roche Diagnostics, Germany), 100 units/mL of penicillin ...
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bioRxiv - Neuroscience 2022Quote: ... An enzyme mixture (0.5 ml) consisting of dispase II (2 mg/ml; Roche, Mannheim ...
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bioRxiv - Immunology 2022Quote: ... 10 μg/ml DNase I and 1.5 mg/ml dispase II (all Roche). The LP lymphocyte-enriched population was harvested from a 40%-80% Percoll (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... digested in R2 buffer containing 0.15mg/ml LiberaseTM and 0.12mg/ml DNAse (Roche) for 45min at 37°C and filtered through 100µm cell strainers.
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bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions of tumors were generated by dicing tumors with a razor blade followed by enzymatic digestion for 30 minutes at 37C in medium containing 1 mg/mL Collagenase D (Roche Diagnostics) and 1μg/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... synovial tissues from three patients with RA were minced and incubated with 1 mg/mL collagenase/dispase (Roche, Indianapolis, IN, USA) in phosphate-buffered saline (pH7.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
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bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion was carried out by resuspending the mixed cell pellet slowly in 1 mL of 50% PEG 1500 (Roche, Basel, Switzerland) at 37°C over the course of 1 minute and incubated for a further 1 minute at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: Frozen fecal samples were diluted in lysis buffer [100mg feces per 1 ml of PBS containing protease inhibitors (Roche, Indianapolis, IN)] ...
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bioRxiv - Molecular Biology 2021Quote: ... Planarians were treated with 5% NAC for 5 minutes and 4% fixation for 20 minutes at room temperature followed by bleaching overnight and treated with proteinase K (1 µg/ml, Roche, 03115844001). After 2 hours of blocking with 1% BSA ...
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bioRxiv - Immunology 2020Quote: Skin biopsy specimens were collected and minced before digestion in 1mL of DMEM with 1 mg/ mL collagenase D (Roche Diagnostics) for 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... at 15-20k cells/ well in Cardiac Mesoderm Patterning Media One made up of CDM containing ROCKi and for all protocols besides the LV cardioids 1 µg/ml of insulin (Roche, #11376497001) plus specific factors depending on cardioid subtype (see below) ...
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bioRxiv - Immunology 2022Quote: ... these tissues were dissected and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cell pellet resuspended in 1 ml of PBS with the addition of a protease inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail, Roche) in order to protect histones from degradation by proteases during the next step of cell lysis ...
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bioRxiv - Genetics 2024Quote: ... cut to very small pieces using a scalpel blade and digested using Collagenase D (Roche, Cat No. 11088858001, 1 mg/ml) /DNase I (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... cecal and colonic tissues were homogenized in 1 mL of PBS −/− supplemented with the cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics). Supernatants were collected by centrifuging the homogenate at 16,000 ×g at 4 °C for 20 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... per sample were washed three times with 1 ml wash buffer (20 mM HEPES at pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1 × Roche complete protease inhibitor) at room temperature and resuspended in 225ul of wash buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were further incubated for 48 h and subsequently subjected to Gentamycin (G418) selection (1 μg/ml, Roche, G418-RO). After 4 days cells were sorted on a fluorescence-activated cell sorter (FACS ...
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bioRxiv - Plant Biology 2023Quote: ... the aerial parts were dipped for 30 s in 1 ml of 0.01% (v/v) Tween-20 aqueous solution containing 50 µM MG132 (Roche Sigma-Aldrich) in DMSO ...
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bioRxiv - Physiology 2023Quote: ... Then the heart was perfused with the same solution containing 1 mg/mL collagenase Type A (Catalog # 9036-06-0; Roche, Germany) and 0.08 mg/mL protease Type XIV (Cat log # 48655321 ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2024Quote: Bacterial cell pellets were resuspended in 50 ml of lysis buffer (50 mM Tris-HCl pH 8, 1 mM DTT, 5% glycerol, protease inhibitor cocktail [Roche 1183615300]). Cells in pellets were lysed by sonication on ice with 10 sec pulses of maximum setting followed by 1 min cooling period ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.25% Triton X-100 and Protease Inhibitor Cocktail (05056489001; Roche)) for 10 min on ice ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 uM ZnCl2 plus EDTA-free Protease Inhibitor Tabs (Roche). Samples were sonicated on ice 1 min at 30% amplitude using a Branson Sonifier and treated with 100 U/ml Benzonase (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5% Triton X-100 containing phosphatases and proteases inhibitors (Roche). For detection of the Huntingtin protein ...
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bioRxiv - Microbiology 2019Quote: ... 0.01% TritonX-100) and then treated with RNase A (Roche) at 0.1 mg/mL during 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% Triton X-100 and 1x protease inhibitor cocktail (Roche). The cell suspension was homogenized ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.05% TritonX-100) supplemented with protease inhibitors (Complete Mini, Roche), layered gently onto 100 μl of hypotonic buffer containing 30% sucrose and centrifuged at 15,000 g at 4°C for 10 min ...
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bioRxiv - Genetics 2022Quote: ... 0.2% Triton X-100) plus proteinase inhibitors (PI) (Complete, Roche), sonicated on ice at 80% power for 8 x 30 s on/ 30 s off with a probe sonicator and centrifuged (13,000 rpm for 20 min 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5% Triton X-100) containing protease and phosphatase inhibitors (Roche). After sonication ...