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Citations for Roche :
1901 - 1950 of 9230 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... after dissection cells were digested for 1.5 hr in Digestion Solution (2mg/ml Collagenase A; 2.4U/ml Dispase II; 10μg/ml DNAse I; in HBSS. All from Roche). After digestion cells were washed with Wash Buffer (0.2% BSA in HBSS ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100, 150mM NaCl, 50mM Tris pH 7.5, 10mM MgCl2, 5mM NaF, 1mM sodium orthovanadate, and Roche EDTA-free protease inhibitor cocktail). Unstimulated control cells were lysed in solution by adding an equal volume of 2x cold lysis buffer for a final volume of 500 μL ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
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bioRxiv - Neuroscience 2021Quote: ... whereas mouse primary cortical cultures and activated T lymphocytes were lysed in lysis buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100) containing protease and phosphatase inhibitors (Roche, New York, NY, USA). Total protein contents were quantified using the BCA assay (Pierce) ...
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bioRxiv - Plant Biology 2019Quote: ... 300 mM sucrose, 1% Triton X-100) containing 1x protease inhibitor cocktail (Pierce, Rockford, IL) and a phosphatase inhibitor (PhosSTOP, Roche Applied Science, Penzberg, Germany). Protein extracts were centrifuged at 1,500 rpm for 5 min and the supernatant was re-centrifuged at 12,000 rpm for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in Tris-Buffered Saline (TBS) + 1% Triton X-100 with EDTA and protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Plant Biology 2022Quote: ... 1/100 cDNA dilutions were analysed using a LightCycler®480 and LightCycler®480 SYBR Green I Master (Roche, Welwyn Garden City, UK) following manufacturer’s instructions and cycling conditions of pre-incubation 95°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... resuspended in 150 μL buffer C (50 mM Tris pH 8, 5 mM EDTA, 1% SDS, 100 mM NaCl, 1x Roche complete mini protease inhibitors) and incubated on ice for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was lysed with 20 ml of Eps8L2 Lysis Buffer (10 mM Tris.HCLpH8, 150 mM NaCl, 1 mMEDTA, lysozyme 0.1 mg/ml, Pefabloc® (Roche) 0.5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads were washed 2 times with lysis buffer and 4 times with washing buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide, 1 mg/ml tRNA from Roche, 5 mM EDTA from Sigma and 50 μg/ml Heparin from Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Genetics 2019Quote: ... DNA was eluted in 100μl of TE + 1%SDS and the beads were washed and eluted a second time with 100μl of TE + 1%SDS + 5 μl of 20mg/mL Proteinase K (Roche). Following overnight incubation at 65°C ...
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bioRxiv - Microbiology 2019Quote: ... The solution was then supplemented with 20 mM MgSO4 and treated with 50 μg mL-1 RNase A (Roche) and 10 μg mL-1 DNase (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: Mouse tracheal epithelial cells were isolated from tracheas digested overnight at 4 °C in Ham’s F12 medium plus pronase (1 mg/ml; Roche). Cells were cultured for 3 h on Primaria plates (Falcon ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellets were resuspended in 1 ml ZAP buffer containing a protease inhibitor (50 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 complete tablet per 50 ml buffer (Roche)) and cells disrupted three times for 45 sec at 6 m/s using an MP Biomedicals™ Fastprep-24 machine ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... the dorsal skin of C57BL/6 neonates was collected and incubated overnight with 1 mg/mL collagenase/dispase (Roche). The dermis and epidermis were separated by incubating the skin with 0.25% trypsin/10 mM EDTA in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... lung samples of infected mice were harvested and homogenized in 1 ml PBS using the MagNA Lyser (Roche Diagnostics). The homogenates were clarified by centrifugation at 15,000 rpm for 5 min ...
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bioRxiv - Microbiology 2022Quote: Fresh pre-cut pancreatic tissue (2 × 2 mm) were digested with a solution of collagenase P in HBSS-1% HEPES (0.75 mg/mL, Roche) at 37°C for 7 min with shaking ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed 2x in PBS + 0,1% Tween-20 (PBS-T) followed by 15 min permeabilization in 1- μg/ml proteinase K (Roche) and 3 washes in PBS-T ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in 3 mL of lysis buffer (160 nM NaCl, 20 mM, Tris-HCl pH7.5, 1 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl) and resuspended in 1 ml Tris-Buffered saline supplemented with protease inhibitor (cOmplete Protease Inhibitor, Roche). Bacterial lysis was done by enzymatic cell wall degradation (30 min at 37°C with 10K/ml mutanolysine ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1× protease inhibitors complete mini (Roche)) ...
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Neuroscience 2019Quote: ... one series of frozen brain slices was homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Genetics 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1 × protease inhibitors complete mini (Roche)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 animals per group were dissected in CMFB (CMF containing 0.5% BSA) and dissociated using 1:50 Liberase (2.5mg/mL, Roche 5401135001) in CMFB at 30°C with agitation at 300 rpm for 30 minutes on an Eppendorf ThermoMixer ...
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bioRxiv - Neuroscience 2020Quote: The frozen half-brains were weighed and homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Right lung lobes were chopped finely and digested in pre-warmed RPMI1640 with ‘collagenase cocktail’ (0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet was resuspended in 1-2 ml of lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; protease inhibitor [Roche]), depending on the size of the cell pellet ...
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bioRxiv - Developmental Biology 2020Quote: ... The remainder of the fracture callus was minced and disassociated manually in Collagenase/Dispase (1 µg/ml, Roche, #10269638001) followed by digestion for 1 hour at 37 degrees ...
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bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% NP40, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1mM Na3VO4, 4 μg/ml leupeptin, one Roche cOmplete™ protease inhibitor tablet ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) (10 mL/g pellet weight) supplemented with DNAse and 1 cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 2 L of culture ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Immunology 2023Quote: Tumors were mechanically homogenized using a Dounce Tissue Homogenizer and enzymatically digested using 0.05mg ml-1 Liberase TL (Roche), 0.2mg ml-1 Dnase I (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...