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Citations for Roche :
1901 - 1950 of 7686 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... genomic DNA was isolated using the High Pure PCR Template Preparation Kit (Roche Life Science). STR DNA genotyping at DSMZ was carried out as described previously using a nonaplex PCR reaction of eight highly polymorphic STR loci plus Amelogenin-based gender determination (Dirks and Drexler 2013) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Paired-end libraries were prepared with the PCR-free KAPA Hyper Prep Kit (KAPA Biosystems). The 150 bp paired-end reads were sequenced with Illumina HiSeq2500 at the Genomics Facility Basel to a mean coverage of 23 (range 18–38 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the synthesized primers for qRT-PCR and KAPA SYBR Fast qPCR Kit (KAPA BIOSYSTEMS) were used to perform qRT-PCR with StepOnePlus (Applied Biosystems) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... cDNA was amplified for 20 cycles using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems). Aliquots of the amplified cDNA were also used for antibody cloning later ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA was extracted using the High Pure PCR Template Preparation Kit (Roche, Germany) according to the manufacturer’s instructions and the 16S rRNA gene of the isolate was amplified by PCR using 27F:AGAGTT TGATCMTGGCTCAG (Wilmotte et al. ...
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bioRxiv - Microbiology 2020Quote: ... A probe for mGFP ORF was generated using the PCR DIG Synthesis Probe Kit (Roche) and primers P35-P36 (Table S1 ...
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bioRxiv - Immunology 2020Quote: ... q-PCR was performed using the Smart SYBRGreen fast Master kit (Roche Diagnostics, Meylan, France) and a CFX Touch RTPCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... PCR was carried out using KAPA SYBR FAST qPCR Kit Master Mix (KAPA BIOSYSTEMS, KK4602) and primer sets (Supplementary Table 1 ...
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bioRxiv - Genomics 2022Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Cancer Biology 2022Quote: ... and DNA was extracted using the High Pure PCR template preparation kit (Roche, Cat# 11796828001) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Genetics 2022Quote: ... cinerea genomic DNA using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared with the purified PCR product by the Kapa DNA Hyper Kit (Roche) together with TruSeq DNA UD indexes for Illumina (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using the KAPA Hyper PCR-free Kit (KR0961, KAPA Biosystems, Wilmington, MA) and sequencing was performed using a NovaSeq S4 XP (Illumina ...
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bioRxiv - Biochemistry 2022Quote: DNA was isolated from tail snips with the High Pure PCR Template Preparation Kit (Roche) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The pool was quantified by quantitative PCR with a Library Quant Kit (Kapa Biosystems, KK4824) and sequenced on an Illumina NovaSeq 6000 with paired-end 150 bp reads.
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bioRxiv - Neuroscience 2023Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Genomics 2024Quote: ... and libraries were constructed using on-bead PCR amplification using the KAPA HyperPlus kit (Roche). The constructed libraries were then sequenced using the Illumina NovaSeq 6000 platform (paired-end 50 bp reads ...
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bioRxiv - Microbiology 2024Quote: Total DNA isolated using the High Pure PCR Template Preparation Kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions was used as a template for nested PCR amplification (detection limit 10-2 parasites per sample ...
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bioRxiv - Cell Biology 2024Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Microbiology 2023Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl RNA eluate was used in a RVFV RT-qPCR using the LightCycler one-tube RNA Amplification Kit HybProbe (Roche, Almere, The Netherlands) in combination with a LightCycler 480 real-time PCR system (Roche ...
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bioRxiv - Pathology 2023Quote: ... The RT-qPCR reactions were performed as recommended by the kit protocol and run on a Roche Light Cycler (Roche, Indianapolis, IN, USA). The limit for cycle of quantification was defined at less than 29 ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from Lipo246 and Lipo815 cell lines and Reverse transcription (RT) reactions were carried out using Transcriptor RT enzyme (Roche Diagnostics, Basel, Switzerland). Nested PCR was performed using a 2-step PCR reaction as previously described(31).
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bioRxiv - Developmental Biology 2019Quote: Non-radioactive RNA in situ hybridization was performed using a digoxigenin (DIG) RNA labeling kit and PCR DIG probe synthesis kit (Roche; http://www.rochediagnostics.us) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The purified indexed PCR library products were quantified using the KAPA Library Quantification Kit from Roche (cat. no. 07960255001, kit code KK4844), normalized to a concentration of 7 nM ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Cancer Biology 2019Quote: ... and Discovery Teal detection systems according to manufacturer’s protocols (Roche). Antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was visualized with ECL light detection reagent (Roche) using Luminescent Image Analyzer (LAS-1000plus ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by Lumi-Light Plus detection reagent (Roche).
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed with the BM-chemiluminescence blotting substrate (Roche) and FusionFx7 imaging system (PeqLab) ...
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bioRxiv - Physiology 2022Quote: ... qPCR analysis was performed using SYBR Green detection (04913914001, Roche) on a QuantStudio 6 system (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... in the Light-Cycler 480 detection System (Roche, Basel, Switzerland). Ct data were calculated with the LightCycler 480 relative quantification software (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11 644 793 001). Maximal lysis of the target cells (= 100% ...
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bioRxiv - Microbiology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was conducted using an in-situ cell death detection kit (Roche, Indianapolis, Indiana, United States) as per the manufacturer’s instructions [31,34] ...
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bioRxiv - Genomics 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay was performed using an in situ cell death detection kit (Fluorescein or TMR; Roche Diagnostics GmbH, Mannheim, Germany). Fixed slides were dried at 37°C for 30 min and rehydrated in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The RT-qPCR was run on a LightCycler 480 (Roche). GAPDH primers were used as an internal control ...
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bioRxiv - Genomics 2021Quote: ... RT-qPCR were performed on the Light Cycler 480II (Roche) with the primers listed in Supplementary Data 6 using Eef1a1 and Hprt as housekeeping controls.
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR was carried out with SYBR Green I (Roche) and SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequent RT-qPCR was performed on a LightCycler96 platform (Roche) based on SARS-CoV-2 N gene RNA amplification using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed on a LightCycler 480 II (Roche) using LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in a LightCycler480 (Roche, Mannheim, Germany) using KAPA SYBR FAST Kit (KK4611 ...
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bioRxiv - Microbiology 2019Quote: ... respectively with and without RT on a LightCycler 480 (Roche).
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bioRxiv - Genetics 2020Quote: ... RT-qPCRs were performed on the Light Cycler 480II (Roche) using Eef1a1 and Hptr as housekeeping genes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... In the following RT qPCR with a Light-Cycler480 (Roche) and Kapa SYBR Fast (Kapa Biosystems ...