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Citations for Roche :
1751 - 1800 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was resuspended in 300 µL of buffer containing 10 mM Na-phosphate buffer (pH 7.0) and EDTA-free Protease inhibitor (Roche). Soluble and membrane fractions were isolated by lysing cells with three freeze-thaw cycles (−20°C to room temperature) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 s off cycle using a total of 30 minutes) in the presence of DNase I (Roche, Basel, CH) and cOmplete EDTA-free protease inhibitor (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclei were isolated from 5 million cells with hypotonic buffer (20 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, protease inhibitors (Roche)) for 15 min on ice ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems, #KK8504) using 8 cycles of PCR ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 1/10 volume of lysis buffer (0.2 M Tris-HCl, pH 8, 10 mg/mL lysozyme, and 1× EDTA-free protease inhibitors; [Roche]), incubated for 30 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... Isolated nuclei were then lysed with lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor complete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Lungs of infected mice were excised and digested enzymatically at 37°C for 60 min in 10 mL of digest buffer (0.34 U/mL LiberaseTM (Roche) and 100 ug/mL DNaseI (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Plant tissues were ground in liquid nitrogen and the powder was suspended in lysis buffer (50 mM Tris-HCl at pH 8.0, 150 mM NaCl, 1 mM phenylmethanesulfonyl fluoride, 10 mM iodoacetamide, and 1X Roche cOmplete mini protease inhibitor cocktail) ...
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bioRxiv - Neuroscience 2024Quote: ... protease inhibitor (1 tablet per 10 ml solution; cOmplete ULTRA Tablets-Mini, F. Hoffmann-La Roche AG, Basel, Switzerland) was added ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 4 ml of a buffer (HEPES 10 mM pH 7.4, 1 mM EDTA, EDTA-free protease inhibitor cocktail from a tablet (Roche)) and lysed by 3 passages through a cell disrupter at 2.4 kbar ...
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bioRxiv - Microbiology 2024Quote: ... Proteases were inactivated by the addition of 1 mM PMSF and 10 ul 1x complete protease inhibitor cocktail (Roche).
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bioRxiv - Biochemistry 2024Quote: Cells were lysed in native lysis buffer (10% glycerol, 25 mM NaCl, 20 mM HEPES pH 7.0, 1% DDM) supplemented with protease inhibitor tablet (Roche), and solubilized by rotating 30 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Beads were washed with 2 × 10 ml of kinase buffer (50 mM Tris-HCl pH 7.6, 10 mM MgCl2, 150 mM NaCl and 1x PhosSTOP (Roche)) ...
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bioRxiv - Plant Biology 2024Quote: ... 10 ng of cDNA was mixed with 7.5 µl of FastStart Universal SYBR Green Master (Rox) (Roche, https://lifescience.roche.com), 0.5 µl of both forward and reverse primers (primer concentration 10 µM ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM iodoacetamide) supplemented with protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, 11836170001, Roche Diagnostics, Germany). Unsolubilized material was pelleted by centrifugation at 7,000 x g for 5 min at 4°C and discarded ...
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bioRxiv - Plant Biology 2024Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM DTT, 0.1% NP-40, and protease inhibitor cocktail [Roche]). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.3 M NaCl, 1 mM MgCl2, 10% glycerol, 1 mM EDTA, 1 mM beta-mercaptoethanol, 0.01% IGEPAL CA-630, 1× Roche cOmplete protease inhibitors ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Amylose Buffer (30 mM HEPES pH 7.6, 0.3 M NaCl, 0.1% CHAPS, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Amylose Buffer supplemented with 20 mM maltose ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 4693132001]) at 4°C and incubated on ice for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR (qPCR) was performed in duplicate reactions of 10 µl volumes (LightCycler 480SYBR Green Master, Roche Holding AG) using 1× SYBR green ...
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bioRxiv - Microbiology 2024Quote: ... cDNA (10 ng per sample) was then analyzed by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche). Reactions were performed in a 384-well format on a QuantStudio 7 Pro at Harvard Medical School’s ICCB-Longwood Screening Facility ...
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bioRxiv - Immunology 2024Quote: ... resuspended in RPMI-1640 (Fisher) supplemented with 10% Fetal Bovine Plasma (FBS) and 20 U/mL deoxyribonuclease I (Roche), concentrated by centrifugation (300 x g for 15 min at 20°C) ...
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bioRxiv - Developmental Biology 2024Quote: ... cut into small pieces and placed into 15 mL tubes containing 10 mL sterile DMEM (without serum) supplemented with 0.5 mg/mL Liberase DL (Roche). Digestion was performed at 37°C for 90 minutes while shaking ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was suspended using Buffer A (25 mM Mops-KOH, pH 7.5, 200 mM NaCl, 10% Glycerol, and cocktail protease inhibitor solution (Roche) with 50-fold dilution) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with cold PBS before resuspending in ChIP shearing buffer (50 mM Tris HCl pH 8.0, 1% SDS, 10 mM EDTA with protease and phosphatase inhibitors, Roche). Samples were sonicated using a Covaris ME220 for 7.5 minutes using the following sonication conditions ...
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bioRxiv - Bioengineering 2024Quote: ... The oligo was ordered preannealed with the reverse complement of the 3′ flanking sequence to facilitate filling in of the bottom strand of the barcode and 5′ flanking sequence by polymerase extension (2.5 μL of preannealed oligos at 10 uM, 12.5 μL KAPA HiFi HotStart ReadyMix (Roche KK2601), and H2O to 25 μL followed by incubation at 98°C for 3 min and 72°C for 30 min) ...
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bioRxiv - Cancer Biology 2024Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 10 cycles with the Kapa HiFi polymerase (Roche). The final libraries were quantitated with Qubit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 500,000 nuclei were cryopreserved in Wash150 (20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM NaButyrate, protease inhibitor cocktail (Roche), 0.5 mM Spermidine ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were thawed and resuspended in 10 µL benzonase buffer (50 mM Tris-HCl pH 8.0, 2 mM MgCl2) with EDTA-free protease inhibitor (Roche, 11873580001 ...
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bioRxiv - Cancer Biology 2021Quote: ... Potential residual cellular phosphatase activity was blocked by incubating HRas samples for 10 min at 37 °C with PhosSTOP (Roche). HRas samples were subsequently incubated for 20 min at 37 °C with 10 mM EDTA and a 15X molar excess of either GMPPNP or GDP ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell suspensions incubated for 10 min at 4°C in SDS lysis buffer containing protease inhibitors (Complete protease inhibitor cocktail, Roche). The remaining procedures were performed as described in previous reports (Carvajal-Gonzalez et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse-transcription reactions were diluted 10-fold with water prior to qPCR which was performed in a Light Cycler 480 instrument (Roche) using the Brilliant III Ultra-Fast SYBR® Green qPCR Mastermix (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... monocytes were harvested using ice-cold lysis buffer (1% Triton X-100, 2% SDS, 150 mM NaCl, 10 mM HEPES, 2 mM EDTA containing protease inhibitor cocktail - Roche). Cell lysates were heated at 100 °C for 5 min in the presence of Laemmli buffer (20% β-mercaptoethanol ...
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bioRxiv - Genetics 2021Quote: ... the color was developed in AP buffer (100 mM Tris-HCl, 100 mM NaCl, 10 mM MgCl2, NBT 4.5 μL/mL [Roche], BCIP 3.5 μL/mL [Roche], pH 9.5).
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bioRxiv - Genetics 2021Quote: ... Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001; Roche). Then DNAse I was inactivated by addition of 1 μl of 25 mM EDTA and incubation at 70°C for 10 min.
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bioRxiv - Microbiology 2020Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
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bioRxiv - Neuroscience 2021Quote: ... and overlaid on top of 29% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 29% Iodixanol, 1x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Immunology 2021Quote: ... sections were rinsed thrice with 1x TBS for 10 minutes each and then stained with DAPI (cat no. 10236276001, Roche) for half an hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... after fixation in 4% formaldehyde and bleaching (Pearson et al., 2009) using blocking solution containing 10% horse serum and western blot blocking reagent (Roche) (King and Newmark ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were digested for either 30 min (48 hpf) or 50 min (72 hpf) with 10 µg/ml proteinase K (Roche), washed with 1x PBT ...
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bioRxiv - Developmental Biology 2020Quote: ... An equal volume of hypertonic lysis buffer (HEPES 20 mM, NaCl2 500 mM, MgCl2 1 mM, Glycerol 10%, DTT 1 mM, 1x Complete protease inhibitor [Roche]) was then added to the lysate ...
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bioRxiv - Cell Biology 2022Quote: ... Freshly harvested cells were incubated for 10 min in ice-cold Hypotonic lysis buffer (HLB) complemented with EDTA-free protease inhibitor cocktail (Roche) and Phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: Right hemispheres of 3 PS19 mice brains of identical ages ranging from 1 to 6 weeks and 1 year old were gently homogenized in 1:10 ratio w/v of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Neuroscience 2022Quote: ... and age-matched controls lacking evident tau pathology were gently homogenized at 4°C in 10 mL of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...