Labshake search
Citations for Roche :
1651 - 1700 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... then incubated for 45 min with constant shaking with “digest buffer” (RPMI; 10% FBS; 1mg/ml collagenase/dispase (Roche); 20μg/ml DNAse 1 (Sigma)) ...
-
bioRxiv - Molecular Biology 2021Quote: (Thawed) Cell pellets were lysed on ice for at least 10 min with 1x protease inhibitors cocktail (PIC; Roche) and 1x quenchers (10 mM NaN3 ...
-
bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl; 10 mM EDTA; 0.1% SDS; 1% Triton X-100; 1% deoxycholate; 1x complete protease inhibitor cocktail [Roche]), clarified and 20 μg total protein were used for SDS-PAGE followed by electroblotting ...
-
bioRxiv - Immunology 2021Quote: B16F10 cells expressing ovalbumin (B16-ova, (Dieterich et al., 2017)) were cultured in DMEM supplemented with 10% FBS and 1.5 mg/ml G418 (Roche).
-
bioRxiv - Molecular Biology 2021Quote: ... washed twice in cold PBS and resuspended in lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.1, supplemented with protease inhibitor cocktail, #11873580 001, Roche). Chromatin fragmentation was performed using Soniprep 150 to produce a distribution of fragments enriched between 300 and 400 bp ...
-
bioRxiv - Immunology 2020Quote: ... The first PCR was performed in 10 μl and consisted of 1x KAPA HiFi Hot Start Ready Mix (Roche), each 16S rRNA primer at 0.2 μM and 3-μl DNA ...
-
bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
-
bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP and 10 % glycerol) supplemented with 2x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
-
bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
-
bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were harvested and re-suspended in buffer A supplemented with 10 μM ZnCl2 and protease inhibitor (Complete, Roche). Cells were then homogenized by sonication and the crude extract was centrifuged for 30 min at 18,000□×□g ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 min), resuspended in lysis buffer (10% glycerol, 20 mM HEPES pH 7.4, 200 NaCl, cOmplete protease inhibitor mixture (Roche), 1 mg/mL DNAse I (Roche)) ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer2 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche), 0.5 % IGEPAL CA-630 ...
-
bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were harvested and solubilized in the lysis buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol) plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The bacterial pellet was resuspended in lysis buffer (7.5 mL PBS supplemented with 1% Triton, 10 μg/mL DNaseI, EDTA-free Protease Inhibitor Cocktail (Roche)) and was incubated for 1 h on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
-
Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche complete EDTA-free protease inhibitor ...
-
bioRxiv - Genetics 2022Quote: ... resuspended in lysis buffer (25 mM Tris-HCl at pH 7.4, 150 mM NaCl, 10% glycerol, 1% NP-40, Roche Complete EDTA-free protease inhibitor cocktail ...
-
bioRxiv - Immunology 2022Quote: ... the cells were lysed with ChIP-Lysis buffer (10 mM EDTA, 1% [w/v] SDS, 50mM Tris-HCl, pH 7.5, 1 tablet Protease Inhibitor Cocktail [#11836170001; Roche, Mannheim ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were ruptured using a beadbeater with 1.9g of glass beads either in SDS buffer (1X phosphate buffered saline, 10% Glycerol, 2mM EDTA, 2mM DTT, Roche protease inhibitor cocktail ...
-
bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
-
bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
-
bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
-
bioRxiv - Neuroscience 2022Quote: ... Biotinylated cells were scraped into lysis buffer (10 mM Tris pH 7.6, 1% Triton X-100, 150 mM NaCl, protease inhibitor (complete, without EDTA (Roche), ½ tablet per 20 mL) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in lysis buffer (150 mM NaCl, 50 mM HEPES pH 7.6, 1 mM EDTA, 2 mM MgCl2, 1% Triton X, 10% Glycerol, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
-
bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche]). Cell lysis was obtained by a mechanical-pestle and sonication ...
-
bioRxiv - Molecular Biology 2022Quote: ... then resuspended in cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C and incubated on ice for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell pellets were thawed on ice and resuspended in cold lysis buffer (500mM NaCl, 50mM Tris-HCl pH 8.0, 10mM Imidazole, 10% Glycerol) + 1x cOmpleteTM protease inhibitor tablet (Roche) and sonicated on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were harvested by centrifugation at 700xg and resuspended in 10-15mL lysis buffer (150 mM KCl, 1mM MgCl2, 0.1% Tween-20, protease inhibitor (Complete EDTA-free tablets, Roche)) per liter of culture ...
-
bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
-
bioRxiv - Plant Biology 2024Quote: ... were ground in liquid N2 and 100 µL extraction buffer added (125 mM Tris-Cl pH 8.8, 1% SDS, 10% glycerol, 1 mM PMSF and Complete Protease Inhibitor [Roche]). Samples were centrifuged at 7000 g for 10 min and supernatant collected ...
-
bioRxiv - Neuroscience 2023Quote: ... The heads were crushed on ice using a pestle in 0.5% NP-40 lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM EDTA, pH 7.5) supplemented with protease inhibitors (Roche). The resulting lysates were clarified by centrifugation and the supernatants were collected ...
-
bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
-
bioRxiv - Microbiology 2024Quote: ... Frozen cell pellets were resuspended in 600 μL of 1X IPOD lysis buffer (10 mM Tris HCl, pH 8.0; 50 mM NaCl) with 1x protease inhibitors tablet (Roche Complete Mini ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 300 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 50 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
-
bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
-
bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
-
bioRxiv - Cancer Biology 2024Quote: ... Total volume of 20 μl reaction mix contained 10 μl LightCycler® 480 SYBR Green I Master Mix (Roche), 2 μl 2.5 mM primer (F+R) ...
-
bioRxiv - Cancer Biology 2024Quote: ... resuspended in lysis buffer (20 mM HEPES, 500 mM NaCl and 10 mM imidazole, pH 7.5) with cOmplete protease inhibitor Cocktail (Roche), and lysed by a sonicator (Q125 ...
-
bioRxiv - Cancer Biology 2023Quote: ... lysed in Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS) supplemented with Complete® protease inhibitors (Roche), and sonicated before protein quantification (DC BioRad Protein Assay Cat No ...
-
bioRxiv - Neuroscience 2023Quote: ... skin tissues were homogenized at a 10% (w/v) concentration in a lysis buffer containing 2 mM CaCl2 and 0.25% (w/v) collagenase A (Roche) in Tris-Buffered Saline (TBS) ...